Circuit motifs for spatial orientation behaviors identified by neural network optimization.

Spatial orientation behavior is universal among animals, but its neuronal basis is poorly understood. The main objective of the present study was to identify candidate patterns of neuronal connectivity (motifs) for two widely recognized classes of spatial orientation behaviors: hill climbing, in which the organism seeks the highest point in a spatial gradient, and goal seeking, in which the organism seeks an intermediate point in the gradient. Focusing on simple networks of graded processing neurons characteristic of Caenorhabditis elegans and other nematodes, we used an unbiased optimization algorithm to seek values of neuronal time constants, resting potentials, and synaptic strengths sufficient for each type of behavior. We found many different hill-climbing and goal-seeking networks that performed equally well in the two tasks. Surprisingly, however, each hill-climbing network represented one of just three fundamental circuit motifs, and each goal-seeking network comprised two of these motifs acting in concert. These motifs are likely to inform the search for the real circuits that underlie these behaviors in nematodes and other organisms.

Nucleotide substitutions and the evolution of duplicate genes.

This paper describes software created to search for and analyze pairs of duplicate genes within a genome. The process is based on a program that uses aligned amino acid sequences to generate a corresponding alignment of the underlying nucleotide sequences and perform a codon by codon comparison of the nucleotides. Observed numbers of nucleotide substitutions can be used to make inferences about the ages of gene duplication events and the effects of natural selection acting on duplicate genes.

The evolutionary fate and consequences of duplicate genes.

Gene duplication has generally been viewed as a necessary source of material for the origin of evolutionary novelties, but it is unclear how often gene duplicates arise and how frequently they evolve new functions. Observations from the genomic databases for several eukaryotic species suggest that duplicate genes arise at a very high rate, on average 0.01 per gene per million years. Most duplicated genes experience a brief period of relaxed selection early in their history, with a moderate fraction of them evolving in an effectively neutral manner during this period. However, the vast majority of gene duplicates are silenced within a few million years, with the few survivors subsequently experiencing strong purifying selection. Although duplicate genes may only rarely evolve new functions, the stochastic silencing of such genes may play a significant role in the passive origin of new species.