RESUMO
This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
Assuntos
RNA de Plantas/isolamento & purificação , Arabidopsis/química , Arabidopsis/genética , Northern Blotting , Precipitação Química , Etanol , Análise de Sequência com Séries de Oligonucleotídeos , Fenol , RNA de Plantas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Acetato de SódioRESUMO
A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible with the small quantities of available tissue often associated with this species and was originally intended for Western blot preparations. Samples extracted using this method can be quantitated directly using a commercially available kit.