RESUMO
The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
Assuntos
Núcleo Celular/metabolismo , Proteínas Nucleares/metabolismo , Receptores Citoplasmáticos e Nucleares/metabolismo , Fosfatase Alcalina/antagonistas & inibidores , Sequência de Aminoácidos , Antígenos Transformantes de Poliomavirus/química , Ligação Competitiva/efeitos dos fármacos , Biotina/química , Núcleo Celular/efeitos dos fármacos , Colorimetria , Indicadores e Reagentes , Indóis/química , Dados de Sequência Molecular , Proteínas Nucleares/efeitos dos fármacos , Peptídeos/síntese química , Peptídeos/farmacologia , Ligação Proteica , Receptores Citoplasmáticos e Nucleares/efeitos dos fármacos , Estreptavidina , alfa CarioferinasRESUMO
"The purpose of this article is to...[analyze] the relative importance of internal and external factors on the demand for skilled immigration visas to Australia. Our objectives are to determine how the size of the pool of potential migrants is influenced by factors such as relative economic conditions and U.S. and Canadian immigration policies and to determine what implications these factors have on the relative quality (skill level) of potential migrants to Australia. Our results indicate that the demand for skilled immigration visas to Australia is related to the number of immigrants accepted by the United States and Canada as well as employment possibilities in Australia. We do not find a relationship between U.S. and Canadian policy and the relative quality of the applicant pool."
Assuntos
Economia , Emigração e Imigração , Emprego , Mão de Obra em Saúde , Política Pública , Migrantes , América , Austrália , Canadá , Demografia , Países Desenvolvidos , América do Norte , Ilhas do Pacífico , População , Dinâmica Populacional , Classe Social , Fatores Socioeconômicos , Estados UnidosRESUMO
The perinatal deaths which occurred during 1973 in the Harare perinatal service were analysed in relation to their obstetric associations. Seventy-one per cent of all the deaths were associated with just five obstetric complications, namely asphyxia in laubour, preterm delivery, minor antepartum haemorrhage, unexplained intra-uterine death and disproportion. It is suggested that asphyxial deaths occurring labour will only be eliminated when continous cardiotocographic monitoring becomes standard practice for all patients. Until then, selected cardiotocography will remain as the main diagnostic aid. Its value will be improved by reviewing the principles of selection. The potential exists to eliminate preterm delivery by the use of beta-adrenergic stimulant drugs. It is suggested that the rational application of this potential would reduce perinatal mortality from preterm delivery. The unexplained intra-uterine deaths and those associated with a minor antepartum haemorrhage presented major problems of understanding and therefore management. These are discussed. Deaths associated with disproportion occurred in 0,10% of booked patients and 3,22% of unbooked patients. The management of the booked patient is satisfactory, and improvement in perinatal mortality will only be achieved by decreasing the number of unbooked patients.
