Estimation of the relative sensitivity of the comparative tuberculin skin test in tuberculous cattle herds subjected to depopulation.

Bovine tuberculosis (bTB) is one of the most serious economic animal health problems affecting the cattle industry in Great Britain (GB), with incidence in cattle herds increasing since the mid-1980s. The single intradermal comparative cervical tuberculin (SICCT) test is the primary screening test in the bTB surveillance and control programme in GB and Ireland. The sensitivity (ability to detect infected cattle) of this test is central to the efficacy of the current testing regime, but most previous studies that have estimated test sensitivity (relative to the number of slaughtered cattle with visible lesions [VL] and/or positive culture results) lacked post-mortem data for SICCT test-negative cattle. The slaughter of entire herds ("whole herd slaughters" or "depopulations") that are infected by bTB are occasionally conducted in GB as a last-resort control measure to resolve intractable bTB herd breakdowns. These provide additional post-mortem data for SICCT test-negative cattle, allowing a rare opportunity to calculate the animal-level sensitivity of the test relative to the total number of SICCT test-positive and negative VL animals identified post-mortem (rSe). In this study, data were analysed from 16 whole herd slaughters (748 SICCT test-positive and 1031 SICCT test-negative cattle) conducted in GB between 1988 and 2010, using a bayesian hierarchical model. The overall rSe estimate of the SICCT test at the severe interpretation was 85% (95% credible interval [CI]: 78-91%), and at standard interpretation was 81% (95% CI: 70-89%). These estimates are more robust than those previously reported in GB due to inclusion of post-mortem data from SICCT test-negative cattle.

Diffuse light increases metabolic activity in the lateral geniculate nucleus, visual cortex, and superior colliculus of the cone-dominated ground squirrel visual system.

Ground squirrels were monocularly exposed to either steady- or flashing-diffuse light for 45 min following an injection of 14C 2-deoxyglucose (2-DG). Autoradiographic analysis indicated greater metabolic activity in the lateral geniculate nucleus, visual cortex and superior colliculus (SC) of the hemisphere lying contralateral to and receiving input from the diffusely stimulated eye (covered by a white mask), than in the corresponding regions of the other hemisphere receiving input from the occluded eye (black mask). The diffuse light results for the cortex and colliculus of the diurnal ground squirrel are different from those for the nocturnal rat. In the rat visual cortex, there is no difference between metabolic activity under conditions of diffuse light (steady or flashing) and under darkness. In the rat SC, although flashing-diffuse light increases metabolic activity (as is the case for the squirrel), steady-diffuse light decreases it to a level below that which occurs in darkness. The cortex and colliculus differences in 2-DG response to diffuse light between the ground squirrel and rat were attributed to differences in the operations of their respective cone- and rod-dominated visual systems.