RESUMO
Severe endocarditis with myonecrosis, moderate to severe pleural and pericardial effusions, and mild ascites were found on necropsy in 3 alpacas. Mycoplasma hominis ssp. was detected on polymerase chain reaction (PCR) of fresh affected endocardial tissue in 1 alpaca.
Mycoplasma hominisssp. associé à l'endocardite avec une nécrose du myocarde chez un alpaga(Vicugna pacos)au Manitoba en 2011. Une endocardite grave accompagnée de myonécrose, d'effusions pleurales et péricardiques de modérées à graves et d'ascite légère a été découverte à la nécropsie chez 3 alpagas. Mycoplasma hominis ssp. a été détecté à l'amplification en chaîne par polymérase d'un tissu endocardiaque touché chez 1 alpaga.(Traduit par Isabelle Vallières).
Assuntos
Endocardite Bacteriana/veterinária , Infecções por Mycoplasma/veterinária , Mycoplasma hominis , Miocárdio/patologia , Necrose/veterinária , Animais , Endocardite Bacteriana/microbiologia , Endocardite Bacteriana/patologia , Feminino , Masculino , Infecções por Mycoplasma/microbiologia , Infecções por Mycoplasma/patologia , Necrose/microbiologia , Necrose/patologiaRESUMO
A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.
Assuntos
Infecções por Vírus de DNA/veterinária , Vírus de DNA/classificação , Vírus de DNA/isolamento & purificação , Doenças dos Peixes/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Conservação dos Recursos Naturais , Infecções por Vírus de DNA/mortalidade , Infecções por Vírus de DNA/patologia , Infecções por Vírus de DNA/virologia , DNA Viral/genética , Doenças dos Peixes/mortalidade , Doenças dos Peixes/patologia , Peixes , Regulação Viral da Expressão Gênica/fisiologia , Lagos , Manitoba , Anotação de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Proteínas Virais/genética , Proteínas Virais/metabolismoRESUMO
The discovery of the parasite Toxocara vitulorum in bison calves in the province of Manitoba, Canada is discussed. This parasite is more commonly found in the small intestines of bovid calves living in tropical and subtropical regions of the world. This is the first time that Toxocara vitulorum has been reported from hosts in Canada.
