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Biochim Biophys Acta ; 1828(2): 309-16, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23103506

RESUMO

Based on the fact that quercetin (QUE) and resveratrol (RES) induce a synergic inhibition of the adipogenesis and increase apoptosis in adipocytes, and that sodium deoxycholate (SDC) has necrotic effects, the nanoencapsulation of QUE and RES into SDC-elastic liposomes is proposed as a new approach for dissolving the subcutaneous fat. The concentration of constituents and the effect of the drug incorporation into cyclodextrin inclusion complexes on the stability of QUE/RES-loaded liposomes were studied. The best liposomal formulation reduced the use of phosphatidylcholine and cholesterol in 17.7% and 68.4%, respectively. Liposomes presented a mean diameter of 149nm with a polydispersion index of 0.3. The zeta potential of liposomes was slightly negative (-13.3mV) due to the presence of SDC in the phospholipid bilayer. Encapsulation efficiency of QUE and RES into liposomes was almost 97%. To summarize, QUE/RES-loaded elastic liposomes are stable and suitable for subcutaneous injection, thereby providing a new strategy for reducing subcutaneous fat.


Assuntos
Lipossomos/química , Quercetina/química , Estilbenos/química , Adipócitos/efeitos dos fármacos , Físico-Química/métodos , Colesterol/química , Cromatografia Líquida de Alta Pressão/métodos , Ciclodextrinas/química , Ácido Desoxicólico/farmacologia , Sistemas de Liberação de Medicamentos , Desenho de Fármacos , Elasticidade , Cinética , Microscopia Eletrônica de Varredura/métodos , Nanopartículas/química , Nanotecnologia/métodos , Fosfatidilcolinas/química , Resveratrol , Solubilidade , Estilbenos/administração & dosagem , Fatores de Tempo
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