Concordance of the vestibular bone thickness at the level of point a between teleradiography and cone beam computed tomography / Concordancia del espesor óseo vestibular a nivel del punto a entre telerradiografía y tomografía computarizada de haz cónico

Objective: The aim of this study was to determine the concordance of the vestibular bone thickness measured at the level of point A between Teleradiography and Cone Beam Computed Tomography (CBCT). Materials and Methods: This study consisted of a cross-sectional analytical design of concordance that evaluated the teleradiographies and CBCTs of 32 patients. The measurements were performed by three evaluators, specialists in orthodontics. Two of them measured the CBCTs and one evaluated the teleradiographs. The concordance of both tests was determined using the Concordance Correlation Coefficient. Results: When evaluating the value of the vestibular bone thickness at the level of point A between the CBCT and the teleradiography, it was observed that the mean value of the absolute difference between the two was 0.95±0.74, 95%CI [0.68­1.22], being statistically significant (p=0.0027). When the concordance between both tests was analyzed, it was observed that it was poor (CCC=0.204 95%CI [0.014­0.394]), although statistically significant (p<0.00001). Conclusions: It was possible to conclude that there is no concordance in the measurement of the vestibular bone thickness at the level of Point A between the Teleradiography and the CBCT.

Objetivo: El objetivo de este estudio fue determinar la concordancia del espesor óseo vestibular medido a nivel del punto A entre la Telerradiografía y la Tomografía computarizada de haz cónico (CBCT). Materiales y Métodos: Esta investigación presentó un diseño analítico transversal de concordancia en el que se evaluaron las telerradiografías y CBCT de 32 pacientes. Las mediciones fueron realizadas por tres evaluadores especialistas en ortodoncia, dos de ellos midieron los CBCT y uno las telerradiografías. La concordancia de ambos exámenes fue medida mediante Coeficiente de Correlación de Concordancia. Resultados: Al evaluar el valor del grosor óseo vestibular a nivel del punto A entre el CBCT y la telerradiografía, se observó que el valor promedio de diferencia absoluta entre ambos fue de 0,95±0,74 IC95% [0,68­1,22], siendo estadísticamente significativas (p=0,0027). Cuando se analizó la concordancia entre ambos exámenes se observó que esta fue pobre (CCC=0,204 IC95 % [0,014­0,394]), aunque estadísticamente significativa (p<0,00001). Conclusión: Se pudo concluir que no existe concordancia en la medición del espesor óseo vestibular medido a nivel del Punto A entre la Telerradiografía y el CBCT.

First Report of Anthracnose Caused by Colletotrichum asianum on Mango (Mangifera indica) in Peru.

Mangoes (Mangifera indica L.) are one of the most important export fruits in Peru and anthracnose, caused by several species in the Colletotrichum gloeosporioides species complex (CGSC), is one of their main postharvest diseases (Alvarez et al. 2020). Balsas is the major mango producing district in the Amazonas department, where farmers practice intercropping in orchards mostly of less than 5 ha (Cabezudo Cerpa 2022). In July 2021, mango fruits cv. Kent with anthracnose were detected at an incidence of 55 to 80% during postharvest in Balsas. Symptoms included sunken dark brown lesions with appearance of orange conidiomata at advanced stages of the disease. We collected two samples of infected mangoes from a farm located at 6°51'01" S, 77°59'48" W (1088 m.a.s.l.). One axenic culture (INDES-AM1) was obtained from a hyphal tip of a monosporic colony and cultivated on PDA medium at 25 °C in the dark. The growing rate of the colony was 8.1 mm.day-1. Conidia were hyaline, guttulate, unicellular and cylindrical with narrowing center, with dimensions of 15.8 to 23.5 × 4.5 to 7.6 µm (mean = 18.6 ± 0.03 × 6.0 ± 0.02 µm, SE, n = 50), consistent to the CGSC (Weir et al. 2012). Appressoria were dark brown, and ovoid to slightly irregular in shape, ranging from 5.3 to 10.1 × 4.7 to 8.3 µm (mean = 7.9 ± 0.02 × 6.0 ± 0.02 µm, SE, n = 50). Koch's postulates were fulfilled on mature mango fruits of the same cultivar and from the same district. Mangoes were washed with detergent and left to dry before inoculation. PDA-mycelial plugs of 0.5 cm wide were transferred on two different locations of two fruits, with four replicates. One location was previously wounded with five needle punctures of 3 mm depth. The inoculated fruits were maintained in a moist chamber at ambient light and temperature (18.9 ± 0.5 °C, SE). Symptoms appeared three-to-five days post inoculation (dpi), and the superficial diameter of the lesions were 8.3 ± 1.5 and 3.6 ± 2 mm with the invasive and the superficial inoculation approaches, respectively, at five dpi. Lesions were very similar to original symptoms. Macro and micromorphological characteristics of the re-isolated fungal colonies were the same as isolate INDES-AM1. Molecular identification of the pathogen was carried out following Weir et al. (2012). Total DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega Corp., Madison, Wisconsin) and the ribosomal internal transcribed spacer (ITS), and partial sequences of the chitin synthase (CHS1), actin (ACT), ß-tubulin 2 (TUB2), calmodulin (CAL), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear genes were sequenced (Accession numbers: OP425395, OP440444, OP440442, OP440443, OP555062, OP555063). ITS, CHS1, ACT, TUB2, CAL, and GAPDH sequences were 98.6, 100, 100, 99.5, 100, and 99.08% identical to Colletotrichum asianum type strain ICMP 18580 sequences, respectively. Additionally, a bootstrapped maximum likelihood midpoint-rooted phylogeny with a multilocus dataset with the six sequences from reference strains of C. asianum and closely related species within the CGSC revealed that strain INDES-AM1 is C. asianum. This species has been found causing anthracnose on M. indica in at least 15 different countries in Africa, America, Asia, and Oceania (Weir et al. 2012). It was originally described from coffee and has multiple other hosts (Prihastuti et al. 2009; Lima et al. 2015). To the best of our knowledge, this is the first report of C. asianum infecting mangoes in Peru.

[Incidence of COVID-19 in health workers. Work activity and home protection]. / Incidencia de COVID-19 en trabajadores de salud. Actividad laboral y resguardo domiciliario.

Background: The COVID-19 pandemic has had repercussions in the social, economic, psychological and health spheres, for which risk and exposure groups have been identified. Health workers are distinguished from the rest of the population by constant contact with patients carrying the SARS-CoV-2 virus. Objetive: To determine the difference in the incidence of COVID-19 in health workers who remain in work activity and those who shelter at home. Material and methods: Cohort study in health workers from March 2020 to January 2021. Two groups were integrated, the exposed group made up of workers who remained working in the health unit (n = 4650) and the non-exposed group, those who carried out home protection (n = 337). When the groups were integrated, none had a diagnosis of COVID-19, they were followed up for 11 months and when COVID-19 was diagnosed, it was done with a PCR test. Statistical analysis included incidence rate, confidence intervals, Chi square, relative risk, and confidence interval for relative risk. Results: The incidence of COVID-19 in workers in home shelter was 39.8% (95% CI: 34.6-45.0) and in workers who remained in the medical unit 16.0% (95% CI: 14.9-17.1). The relative risk was 0.40 (95% CI: 0.34-0.47) for workers who remained working in the health unit. Conclusion: The incidence of COVID-19 was higher in health workers with home protection than in those who continued with normal activities in the medical unit.

Introducción: la pandemia por COVID-19 ha repercutido en el ámbito social, económico, psicológico y de la salud, por lo que se han identificado grupos de riesgo y de exposición. Los trabajadores de la salud se distinguen del resto de la población por el contacto constante con pacientes portadores del virus SARS-CoV-2. Objetivo: determinar la diferencia de incidencia de COVID-19 en trabajadores de la salud que permanece en actividad laboral y los que se resguardan en domicilio. Material y métodos: estudio de cohorte en trabajadores de la salud de marzo 2020 a enero 2021. Se integraron dos grupos: el grupo expuesto compuesto por trabajadores que permanecieron laborando en la unidad de salud (n = 4650) y el grupo no expuesto, que realizaron resguardo domiciliario (n = 337). Cuando se integraron los grupos ninguno tenía diagnóstico de COVID-19, se siguieron durante 11 meses y cuando se diagnosticó COVID-19 se realizó con prueba de PCR. El análisis estadístico incluyó tasa de incidencia, Intervalos de confianza, Chi cuadrada, riesgo relativo e intervalo de confianza para riesgo relativo. Resultados: la incidencia de COVID-19 de trabajadores en resguardo domiciliario fue de 39.8% (IC95%: 34.6-45.0) y en trabajadores que permanecieron en la unidad médica de 16.0% (IC95%: 14.9-17.1). El riesgo relativo fue de 0.40 (IC95%: 0.34-0.47) para trabajadores que permanecieron laborando en la unidad de salud. Conclusión: la incidencia de COVID-19 fue superior en trabajadores de la salud con resguardo domiciliario que en aquellos que continuaron con actividades normales en la unidad médica.

Uso del láser terapéutico en el control del dolor en ortodoncia / Use of laser therapy in pain control in orthodontics

Se realizó una revisión de los últimos años de la literatura para analizar la información existente respecto al uso de láser terapéutico en el control del dolor durante el tratamiento de ortodoncia. Se encontró que el láser terapéutico puede ser efectivo como coayudante en el control del dolor sin embargo las diferencias no son significativas.

There is realized a review of last years of the literature to analyze the existing information with regard to the use of therapeutic laser in the control of the pain during the treatment of orthodontics.

Pensamiento y acciones en salud / Thought and actions in health

El presente trabajo encomendado al Grupo 4 del Módulo III Salud Pública del Curso, tiene como misión relacionar el pensamiento y acción de salud, por lo que presenta la exposición de dicho esquema interpretativo, relacionándolo con el diagnóstico del sistema y la participación de la Policía Nacional en el Sistema Nacional de Salud; asimismo detalla las variables que muestran las condiciones de salud de la población; pensamientos; análisis e interpretación; acciones de salud. Contiene los esquemas interpretativos entre: a. Constitución de la OMS, Declaración de Alma Ata, Declaración Conferencia Internacional de Promoción de la Salud; b. Constituciones: Chile, Colombia, Perú