Impact of hormone therapy on the bone density of women with premature ovarian insufficiency: A systematic review.

INTRODUCTION: Women with premature ovarian insufficiency (POI) are exposed to a long period of estrogenic deficiency, which potentially brings higher health risks, especially regarding bone health. We performed a systematic review of the literature to evaluate the effect of hormone therapy (HT) on bone mineral density (BMD) in women with POI. MATERIALS AND METHODS: A systematic search was performed of the MEDLINE and EMBASE databases up to September 2021. We included studies that analyzed women with idiopathic (spontaneous) POI treated with HT, and those who had BMD evaluated. Analysis of risk of bias of studies selected was performed. RESULTS: We found 335 articles and selected 16 studies according to the inclusion criteria. Most of the studies revealed lower bone density in both the femoral neck and lumbar spine of women with POI compared with healthy women. Bone mass had the tendency to remain stable in women treated with estrogen + progestin therapy. However, in women already with bone mass loss, the therapy - in the doses most frequently used - was not able to revert the loss. Higher doses of estrogen seem to have a positive impact on BMD, as did combined oral contraceptives used continuously. Also, the interruption of HT for longer than one year was linked to significant bone loss. CONCLUSION: Although HT brings clear benefits, further studies are needed to establish its long-term effects, as well as doses and formulations with better protective effects on the bone mass of these women.

Prevalência e Caracterização do Consumo de Tabaco entre Adolescentes de Montes Claros, Minas Gerais, 2019-2020 / Prevalence and Characterization of Tobacco Use Among Adolescents from Montes Claros, Minas Gerais, 2019-2020 / Prevalencia y Caracterización del Consumo de Tabaco en Adolescentes de Montes Claros, Minas Gerais, 2019-2020

Introdução: As doenças tabaco-relacionadas são responsáveis por cinco milhões de óbitos a cada ano. Em 2020, estimou-se que o número de óbitos anuais aumentaria para dez milhões, dos quais 70% ocorreriam nos países em desenvolvimento. Objetivo: Estimar a prevalência do tabagismo e caracterizar o consumo de tabaco entre adolescentes de 12 e de 15 anos na cidade de Montes Claros, Minas Gerais, em 2019-2020. Método: Estudo epidemiológico quantitativo transversal, parte do projeto "Levantamento epidemiológico sobre condições de saúde bucal entre escolares de Montes Claros, Minas Gerais, Brasil", realizado em 2019-2020. A amostra estimada foi de 354 escolares de 12 anos e 355 de 15 anos, calculada entre 4.458 escolares de 12 anos e 4.524 de 15 anos, respectivamente. Os dados foram analisados descritivamente por meio de medidas de tendência central, variância e frequência. Resultados: Houve prevalência do uso de tabaco entre adolescentes alguma vez na vida de 8,6 %, sendo 4,6% relativos ao consumo do cigarro Paiol. Conclusão: A prevalência do consumo encontrada entre os adolescentes é preocupante por causa dos efeitos deletérios à saúde. Conhecer o perfil dos desses usuários pode contribuir para as políticas de saúde coletiva com o objetivo de reduzir o seu uso

Introduction: Tobacco-related diseases are responsible for five million deaths each year. By 2020, the estimate is that the number of annual deaths would increase to ten million, of which 70% would occur in developing countries. Objective: Estimate the prevalence of smoking and characterize tobacco use among adolescents aged 12 and 15 in the city of Montes Claros, Minas Gerais in 2019-2020. Method: Cross-sectional quantitative epidemiological study, part of the project "Epidemiological survey on oral health conditions among students in Montes Claros, Minas Gerais, Brazil", conducted in 2019-2020.The sample consisted of 354 12-year-old and 355 15-year-old adolescents from a population of 4,458 12-year-old and 4,524 15-year-old adolescents, respectively. The data were analyzed through descriptive analysis with measures of central tendency, variance and frequency. Results: There was prevalence of tobacco use in adolescents at some point in their lives of 8.6%, being 4,6% for cigarettes made with pressed tobacco leaves. Conclusion: The prevalence of the use among adolescents is worrying due to the harmful effects to health. Knowing the profile of these users may support health policies aimed to reduce its use

Introducción: Las enfermedades relacionadas con el tabaco son responsables de cinco millones de muertes cada año. Para 2020, la estimación es que el número de muertes anuales aumentaría a diez millones, de los cuales el 70% ocurriría en países en desarrollo. Objetivo: Estimar la prevalencia del tabaquismo y caracterizar el consumo de tabaco en adolescentes de 12 y 15 años de la ciudad de Montes Claros, Minas Gerais, en 2019-2020. Método: Estudio epidemiológico cuantitativo transversal, parte del proyecto "Encuesta epidemiológica sobre condiciones de salud bucal en escolares de Montes Claros, Minas Gerais, Brasil", realizado en 2019-2020. La muestra estimada estuvo conformada por 354 de 12 años y 355 de 15 años, calculada a partir de 4.458 de 12 años y 4.524 de 15 años, respectivamente. Los datos fueron analizados mediante análisis descriptivo que estimaron medidas de tendencia central, variabilidad y frecuencia. Resultados: Esta investigación observó una prevalencia del consumo de tabaco entre los adolescentes en algún momento de su vida del 8,6%, con predominio del 4,6% en el consumo de cigarrillos. Conclusión: La prevalencia de consumo encontrada es preocupante, debido a los efectos nocivos para la salud. Conocer el perfil de los consumidores adolescentes de productos del tabaco puede contribuir a las políticas de salud colectiva para reducir este uso

Physiology, niche characteristics and extreme events: Current and future habitat suitability of a rhodolith-forming species in the Southwestern Atlantic.

Given the ecological and biogeochemical importance of rhodolith beds, it is necessary to investigate how future environmental conditions will affect these organisms. We investigated the impacts of increased nutrient concentrations, acidification, and marine heatwaves on the performance of the rhodolith-forming species Lithothamnion crispatum in a short-term experiment, including the recovery of individuals after stressor removal. Furthermore, we developed an ecological niche model to establish which environmental conditions determine its current distribution along the Brazilian coast and to project responses to future climate scenarios. Although L. crispatum suffered a reduction in photosynthetic performance when exposed to stressors, they returned to pre-experiment values following the return of individuals to control conditions. The model showed that the most important variables in explaining the current distribution of L. crispatum on the Brazilian coast were maximum nitrate and temperature. In future ocean conditions, the model predicted a range expansion of habitat suitability for this species of approximately 58.5% under RCP 8.5. Physiological responses to experimental future environmental conditions corroborated model predictions of the expansion of this species' habitat suitability in the future. This study, therefore, demonstrates the benefits of applying combined approaches to examine potential species responses to climate-change drivers from multiple angles.

Rompimento da barragem em Brumadinho: um relato de experiência sobre os debates no processo de desastres / Dam breaking in Brumadinho: an experience report on the debates in the disaster process

RESUMO O presente artigo teve como objetivo aprofundar discussões do desastre ocorrido em Brumadinho (MG), em janeiro de 2019. A partir da relatoria do seminário 'Desastre da Vale S.A. em Brumadinho: seis meses de impacto e ações', seis alunas do Curso de Especialização em Saúde Pública da Fundação Oswaldo Cruz (Fiocruz) e uma pós-doutoranda da Fiocruz de Minas Gerais realizaram transcrições de falas feitas durante o evento na tentativa de reportar essa vivência mediante lugares de fala distintos. Experiências artísticas retratadas ao longo do texto estabelecem um vínculo com a subjetividade dos atingidos e seu entorno. Portanto, o artigo pretendeu seguir esse caminho.

ABSTRACT The purpose of this article was to deepen discussions on the disaster that occurred in Brumadinho (MG), in January 2019. From the report of the seminar 'Disaster of Vale S.A. in Brumadinho: six months of impact and actions', six students from the Specialization Course in Public Health at Oswaldo Cruz Foundation (Fiocruz) and a postdoctoral fellow at Fiocruz in Minas Gerais performed transcriptions of speeches made during the event in an attempt to report this experience through different places of speech. Artistic experiences portrayed throughout the text establish a link with the subjectivity of those affected and their surroundings. Therefore, the article intended to follow that path.

Physiological damages of Sargassum cymosum and Hypnea pseudomusciformis exposed to trace metals from mining tailing.

The damages of Mariana's mining mud in the physiology of the brown algae Sargassum cymosum and its main epiphytic, the red algae Hypnea pseudomusciformis, were evaluated by controlled essays. Seaweeds were exposed to presence or absence of mud, isolated or in biological association, for 5 and 15 days. Measured parameters were growth rates, biochemical descriptors, and the chemical investigation of concentration and metal profile of the mud dissolved in seawater. Results showed that the highest values for metals were Al > Fe > Mn > Zn in both exposure periods. The mud also affected the growth rate with lethality in both isolated and associative treatments with H. pseudomusciformis after 15 days. According to our redundancy analysis (RDA), the profile and concentration of all metallic elements can induce different physiological responses of the organisms. We were able to observe a higher physiological adaptive ability of S. cymosum against the long-term presence of metals by the synthesis of phenolic compounds, while the deviation of metabolic routes in H. pseudomusciformis can be addressed as the main responsible for its lethality. Moreover, the presence of Hypnea in associative treatments reduces Sargassum's detoxification ability. The present results reinforce the importance of biological interaction studies in a context of physiological resilience against mining mud pollution and mutual influences of species over the individual ability to avoid oxidative stress.

Ecophysiological implications of UV radiation in the interspecific interaction of Pyropia acanthophora and Grateloupia turuturu (Rhodophyta).

Radiation, both photosynthetic active radiation (PAR, l = 400-700 nm) and Ultraviolet (UVR, l = 280-400 nm) is one of the key factors regulating algal distribution in aquatic environments. Pyropia acanthophora and Grateloupia turuturu have been found over upper rocky shore areas in Southern Brazil, occupying the same niche space. The first species is native and the second one is exotic and considered a potential invader of South Atlantic. The aim of the present study was to evaluate the effects of radiation on physiological responses of both species and infer mechanisms that allow their niche competition in the environment. Samples were cultured in the following conditions: associated or separated, and with an addition of PAR, PAR + UVA (PA) and PAR + UVA + UVB (PAB), totalizing six factorial treatments during 5 days of exposure. Photosynthetic responses of Fv/Fm and ETR were daily evaluated. At the beginning and at the end of the experiment, samples were analyzed for pigment content (chlorophyll a and phycobiliproteins), and mycosporine-like amino acids (MAAs), while oxygen evolution was evaluated at the end of the experiment. As the main results, G. turuturu died when cultivated in PAB conditions. P. acanthophora presented higher amounts of chlorophyll a than G. turuturu during the whole experiment. Phycoerythrin and Fv/Fm remained constant in P. acanthophora but diminished for G. turuturu in UV treatments. ETR was higher for samples that were cultivated in associative treatment. The presence of G. turuturu in the same flask enhanced MAA synthesis in P. acanthophora, regardless of radiation condition. In addition, UV radiation can be a factor controlling species distribution and could counteract the spreading of invasive species, like G. turuturu, allowing P. acanthophora survival in upper rocky shore zones of the natural ecological distribution area.

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium.

Sargassum cymosum was exposed to cadmium (Cd) to determine any physiological and ultrastructural effects. To accomplish this, S. cymosum samples were cultivated under photosynthetic active radiation (PAR) and Cd (0, 0.1, 0.2, 0.4 and 0.8 mg L-1) during 7 and 14 days in laboratory-controlled conditions (0 mg L-1 Cd at both exposure times as control). Seaweeds had high retention capacity (over 90 %) for both exposure times. Growth rates showed significant increases by 14 days, especially for 0.1 and 0.4 mg L-1 Cd. Photosynthetic parameters were unaffected by Cd treatments. Chlorophyll contents were present in higher concentrations for all Cd treatments compared to respective control. Carotenoid profile showed significant differences in total composition and proportion of fucoxanthin and ß-carotene, and no lutein was detected at 14 days. Phenolic and flavonoid compounds showed major accumulation at 14 days. Transmission electron microscopy (TEM) analyses presented major alterations in Cd-treated samples, when compared with respective control, in particular disorganization of cell wall fibrils. When compared to respective control samples, multivariate analyses showed disparate and complex interactions among metabolites in Cd-exposed seaweeds, giving evidence of physiological defence response. Thus, it can be concluded that Cd is a stressor for S. cymosum, resulting in physiological and structural alterations related to defence mechanisms against oxidative stress and toxicological effects resulting from long-term metal exposure. However, in the present paper, some observed changes also appear to result from acclimation mechanisms under lower concentration of Cd relative to the tolerance of S. cymosum to experimental conditions.

Effects of copper and lead exposure on the ecophysiology of the brown seaweed Sargassum cymosum.

The effects of the heavy metals copper (Cu) and lead (Pb) on Sargassum cymosum were evaluated by determining uptake capacity, growth rates, photosynthetic efficiency, contents of photosynthetic pigments and phenolic compounds, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity, and morphological and cellular changes. S. cymosum was cultivated with Cu and Pb separately and combined at concentrations of 10, 25, and 50 µM for 7 days in laboratory-controlled conditions. Seaweeds under Cu treatment showed the highest biosorption capacity, and growth rates were significantly reduced compared to the control. The photosynthesis/irradiance curves showed alterations in kinetic patterns in the metal-treated samples. Specifically, Cu treatment alone inhibited electron transport rate (ETR) response, while Pb alone induced it. However, samples treated with both Cu and Pb (Cu + Pb) showed inhibition in ETR. The total amount of pigments increased relative to control. Light microscopy showed an increase in phenolic compounds, with physodes migrating towards cortical cells. Scanning electronic microscopy revealed alterations in the typical rough surface of thallus, when compared with control, especially for Pb treatments. Based on these results, it could be concluded that Cu and Pb are stress factors for S. cymosum, promoting alterations in seaweed metabolism and stimulating protective mechanisms against oxidative stress. However, the high bioaccumulation capacity of both heavy metals indicates a possible application for S. cymosum as a biosorbent agent for contaminated wastewater when metals are in low concentrations.

Effects of ultraviolet radiation (UVA+UVB) and copper on the morphology, ultrastructural organization and physiological responses of the red alga Pterocladiella capillacea.

The effect of ultraviolet (UV) radiation and copper (Cu) on apical segments of Pterocladiella capillacea was examined under two different conditions of radiation, PAR (control) and PAR+UVA+UVB (PAR+UVAB), and three copper concentrations, ranging from 0 (control) to 0.62, 1.25 and 2.50 µm. Algae were exposed in vitro to photosynthetically active radiation (PAR) at 70 µmol photons m(-2)  s(-1) , PAR + UVB at 0.35 W m(-2) and PAR +UVA at 0.70 W m(-2) during a 12-h photocycle for 3 h each day for 7 days. The effects of radiation and copper on growth rates, content of photosynthetic pigments and photosynthetic performance were analyzed. In addition, samples were processed for light and transmission electron microscopy. The content of photosynthetic pigments decreased after exposure to radiation and Cu. Compared with PAR radiation and copper treatments modified the kinetics patterns of the photosynthesis/irradiance curve. The treatments also caused changes in the ultrastructure of cortical and subcortical cells, including increased cell wall thickness and accumulation of plastoglobuli, as well as changes in the organization of chloroplasts. The results indicate that the synergistic interaction between UV radiation and Cu in P. capillacea, led to the failure of protective mechanisms and causing more drastic changes and cellular imbalances.

The effect of cadmium under different salinity conditions on the cellular architecture and metabolism in the red alga Pterocladiella capillacea (Rhodophyta, Gelidiales).

The in vitro effect of cadmium (Cd) on apical segments of Pterocladiella capillacea was examined. Over a period of 7 days, the segments were cultivated with the combination of different salinities (25, 35, and 45 practical salinity units) and Cd concentrations, ranging from 0.17 to 0.70 ppm. The effects of Cd on growth rates and content of photosynthetic pigments were analyzed. In addition, metabolic profiling was performed, and samples were processed for microscopy. Serious damage to physiological performance and ultrastructure was observed under different combinations of Cd concentrations and salinity values. Elementary infrared spectroscopy revealed toxic effects registered on growth rate, photosynthetic pigments, chloroplast, and mitochondria organization, as well as changes in lipids and carbohydrates. These alterations in physiology and ultrastructure were, however, coupled to activation of such defense mechanisms as cell wall thickness, reduction of photosynthetic harvesting complex, and flavonoid. In conclusion, P. capillacea is especially sensitive to Cd stress when intermediate concentrations of this pollutant are associated with low salinity values. Such conditions resulted in metabolic compromise, reduction of primary productivity, i.e., photosynthesis, and carbohydrate accumulation in the form of starch granules. Taken together, these findings improve our understanding of the potential impact of this metal in the natural environment.

Bioabsorption of cadmium, copper and lead by the red macroalga Gelidium floridanum: physiological responses and ultrastructure features.

Heavy metals, such as lead, copper, cadmium, zinc, and nickel, are among the most common pollutants found in both industrial and urban effluents. High concentrations of these metals cause severe toxic effects, especially to organisms living in the aquatic ecosystem. Cadmium (Cd), lead (Pb) and copper (Cu) are the heavy metals most frequently implicated as environmental contaminants, and they have been shown to affect development, growth, photosynthesis and respiration, and morphological cell organization in seaweeds. This paper aimed to evaluate the effects of 50µM and 100µM of Cd, Pb and Cu on growth rates, photosynthetic pigments, biochemical parameters and ultrastructure in Gelidium floridanum. To accomplish this, apical segments of G. floridanum were individually exposed to the respective heavy metals over a period of 7 days. Plants exposed to Cd, Cu and Pb showed discoloration of thallus pigmentation, chloroplast alteration, especially degeneration of thylakoids, and decrease in photosynthetic pigments, such as chlorophyll a and phycobiliproteins, in samples treated with Cd and Cu. Moreover, cell wall thickness and the volume of plastoglobuli increased. X-ray microanalysis detected Cd, Cu and Pb absorption in the cell wall. The results indicate that Cd, Pb and Cu negatively affect metabolic performance and cell ultrastructure in G. floridanum and that Cu was more toxic than either Pb or Cd.

Photoacclimation responses of the brown macroalga Sargassum Cymosum to the combined influence of UV radiation and salinity: cytochemical and ultrastructural organization and photosynthetic performance.

The photoacclimation responses of the brown macroalga Sargassum cymosum were studied to determine its cytochemical and ultrastructural organization, as well as photosynthetic pigments and performance. S. cymosum was cultivated in three salinities (30, 35 and 40 psu) under four irradiation treatments: PAR-only, PAR + UVA, PAR + UVB and PAR + UVA + UVB. Plants were exposed to PAR at 70 µmol photons m(-2) s(-1), PAR + UVB at 0.35 W m(-2) and PAR +UVA at 0.70 W m(-2) for 3 h per day during 7 days in vitro. Growth rate was not significantly affected by any type of radiation or salinity. The amount of pigments in S. cymosum was significantly influenced by the interaction of salinity and radiation treatments. Compared with PAR-only, UVR treatments modified the kinetics patterns of the photosynthesis/irradiance curve. After exposure to UVR, S. cymosum increased cell wall thickness and the presence of phenolic compounds. The number of mitochondria increased, whereas the number of chloroplasts showed few changes. Although S. cymosum showed insensitivity to changes in salinity, it can be concluded that samples treated under four irradiation regimes showed structural changes, which were more evident, but not severe, under PAR + UVB treatment.

Effects of brefeldin A on the endomembrane system and germ tube formation of the tetraspore of Gelidium floridanum (Rhodophyta, Florideophyceae).

Gelidium floridanum W.R. Taylor tetraspores are units of dispersal and are responsible for substrate attachment. This study aimed to examine evidence of direct interaction between germ tube formation and Golgi activity during tetraspore germination of G. floridanum. After release, the tetraspores were incubated with brefeldin A (BFA) in concentrations of 4 and 8 µM over a 6 h period. The controls and treatments were analyzed with light, fluorescence (FM4-64 dye) and transmission electron microscopy. In the control samples, the Golgi bodies were responsible for germ tube formation. In contrast, BFA-treated samples were observed to inhibit spore adhesion and germ tube formation. These tetraspores also showed an increase in volume (≥30 µm width). BFA treatment also resulted in the disassembly of Golgi cisternae and the formation of vesiculated areas of the cytoplasm, blocking the secretion of protein and amorphous matrix polysaccharides. When stained with FM4-64, the control samples showed fluorescence in the apical region of the germ tube, but the treated samples showed an intense fluorescence throughout the cytoplasm. From these results, we can conclude that the germ tube is formed by the incorporation of vesicles derived from Golgi. Thus, vesicle secretion and Golgi organization are basic processes and essential in adhesion and tube formation. By blocking the secretion of protein and amorphous matrix polysaccharides, BFA treatment precluded tetraspore germination.

Avaliação da farmácia caseira no território de Unidade de Saúde de um Serviço de Atenção Primária

O estudo teve como objetivo, avaliar o estoque domiciliar de medicamentos através da identificação dos mais frequentemente armazenados, dos locais de guarda, da presença de vencidos e da caracterização sócio-demográfica da população residente no território de duas Unidades de Saúde de Porto Alegre, RS. Realizou-se um estudo transversal a partir de dados coletados nas visitas domiciliares de rotina do profissional Farmacêutico no período de junho de 2012 a março de 2013. Dentre as 38 famílias visitadas, em 100% houve a presença de medicamentos. Foram encontrados 656 medicamentos, sendo o paracetamol o fármaco mais encontrado nos domicílios (65,8%). O local mais escolhido para o armazenamento, foi a cozinha (45,7%). Dos medicamentos encontrados, 25,5% estavam vencidos ou sem a data de validade. O local mais citado para o descarte de medicamentos foi o lixo comum (14 domicílios). O sexo feminino foi o mais prevalente entre os moradores (62,7%), a faixa etária mais citada foi entre 60 e 80 anos, e a grande maioria não concluiu o Ensino Fundamental. Identificou-se a falta de informação bem como a inadequação das farmácias caseiras, com o mau armazenamento e um acúmulo desnecessário de medicamentos. É importante que os profissionais de saúde orientem a população, através de ações de educação em saúde, para contribuir com mudanças no comportamento e promover o uso racional.