Genotyping of Giardia duodenalis from human and animal samples from Brazil using beta-giardin gene: a phylogenetic analysis.

Giardia duodenalis is one of the major diarrhea agents in human and animals distributed worldwide, and present high levels of genetic diversity, showing seven genotypes: A, B, C, D, E, F, and G. Only Assemblages A and B have been detected in humans and in a wide range of other mammalians hosts, whereas the remaining Assemblages (C-G) are host-specific. Molecular characterization of cysts of human and animal origin are useful to address the co-circulate isolates between these host, and represents an objective means to evaluate zoonotic infection hypothesis. In the present work the G. duodenalis genotypes were characterized by restriction fragment length polymorphisms and DNA sequencing analysis of PCR products of the beta-giardin gene. The cysts were collected in the city of Rio de Janeiro, in Brazil, from a population composed by humans (n=366, 310 children and 56 adults), domestic animals (n=11) from a municipal daycare center in the surroundings of a slum and neighborhood medium-high class domestic animals (n=18). Parasitological exams were developed in human fecal samples. Parasites were found in 60% (186/310) and 66% (37/56) of the samples from children and adults, respectively. Among children's samples, 27.7% (86/310) were positive for G. duodenalis. Only 1.7% (1/56) of the adults was positive for this parasite. In general a total of 87 fecal samples (86 from children and 1 from adult) from all population studied were positive for G. duodenalis, and 62 of these were subjected to molecular analysis using a PCR that amplified a fragment of the beta-giardin gene. Sixty samples were typed as genotype A1, two as genotype A2 and genotype B was not encountered. Among domestic animals samples (n=29), eight (seven dogs and one cat) from the slum community were identified as genotype A1, and all control samples (n=18) were negative in the molecular assay. The host-specific genotypes C, D and, F were not found. In this study we described single case of G. duodenalis infection associated with a child and her dog and both isolates characterized as genotype A1. Despite the low incidence, this data suggest the putative existence of a zoonotic cycle of G. duodenalis in the studied population.

[Maternal and child intestinal parasitism and breast-feeding]. / Aleitamento e parasitismo intestinal materno-infantil.

From March to May 1991, the prevalence of intestinal parasites was detected in 208 children under two-year old, from out patients attending the Public Health Service in Rio de Janeiro. Maternal parasitic infection was simultaneously investigated. Information regarding nurseling's breast-feeding and enteroparasites rates was also recorded. The centrifugal-sedimentation was the method used for stool analysis. Positivity was observed in 12.7% for children and 37.3% for women. Ascaris lumbricoides was the most frequently detected parasite in children under one-year old (4.3%) and mothers (12.7%). The distribution of parasites accordingly to breast-feeding showed a significant statistical difference (p < 0.05) among infants. No exclusively suckled child presented infection. Children whose mothers were infected showed almost two fold risk of infection (1.7). A correlation between enteroparasites and weaned children was observed (r = 0.75). We believe that infected mothers can contribute for parasitic infection of their kids.

[Ascaris lumbricoides in infants: a population-based study in Rio de Janeiro, Brasil]. / Parasitismo por Ascaris lumbricoides em crianças menores de dois anos: estudo populacional em comunidade do Estado do Rio de Janeiro.

Prevalence and intensity of Ascaris lumbricoides infection were analyzed by a cross-sectional study in children addressed in the city of Duque de Caxias, Rio de Janeiro State, from July to December 1997. The study population consisted of children under two years of age (N=387) and mothers of children under one year of age (N=104). Coproparasitologic tests were run using the MIFC and the Kato thick smear (Kato-Katz) methods. Prevalence was 3.3% (95% CI: 1.0-7.8) for children under one year, 30.7% (95% CI: 25.4-36.4) for one-year-olds, and 42.3% (95% CI: 33.0-51.9) for mothers of children under one. Some 38.0% of children under one year of age showed medium-to-heavy infection. These results indicated that ascariasis was an important problem in the first year of life and demonstrated the need for research in the area of maternal and infantile ascariasis.

[Intestinal parasitoses in preschool children of the slum communities of the city of Rio de Janeiro, Brazil]. / Enteroparasitoses em pré-escolares de comunidades favelizadas da cidade do Rio de Janeiro, Brasil.

Prevalence of intestinal parasites was investigated in 1381 low-income children under five years of age from March 1990 to October 1991 in the city of Rio de Janeiro, Brazil. Stool tests were run using the Blagg et al. method. Observed prevalence of infection was 54.5%. The most frequent parasites were Giardia lamblia and Ascaris lumbricoides (25.0% of the children). A significant statistical difference (p<0.05) was demonstrated between age and infection according to the species of parasite studied. Infection rates demonstrated the need for control of parasites in these child.

Ascaris lumbricoides in neonate: evidence of congenital transmission of intestinal nematodes.

Clinical and epidemiological study of a forty-days-old infant with a diarrheic condition and insufficient development led to the coprological diagnosis of ascariasis and possible congenital infection. Specific treatment with levamisole, resulted in clinical and parasitological cure, in addition to gain of weight up to normal levels. Maternal parasitism had been diagnosed two months before labor and proved beyond doubt during the ensuing epidemiological inquiry.