Pollution gradient leads to local adaptation and small-scale spatial variability of communities and functions in an urban marine environment.

Urbanization of coastal habitats, of which harbors and marinas are the paragon, has led to various ecological paradigms about their functioning. Harbor infrastructures offer new hard substrata that are colonized by a wide variety of organisms (biofouling) including many introduced species. These structures also modify hydrodynamism and contaminant dispersal, leading to strong disturbance gradients within them. Differences in sessile community structure have previously been correlated to these gradients at small spatial scale (<100 m). Local adaptation might be involved to explain such results, but as correlation is not causation, the present study aims to understand the causal link between the environmental gradients and community structure through a reciprocal transplant experiment among three sites of a marina (inner, middle, entrance). Our results highlighted strong small-scale spatial variations of contaminants (trace metals, PCB, pesticides, and PAH) in sediments and animal samples which have been causally linked to changes in community composition after transplant. But historical contingency and colonization succession also play an important role. Our results provided strong evidence for local adaptation since community structure, respiration, and pollutant uptake in Bugula neritina, as well as the metabolomes of B. neritina and Ciona intestinalis were impacted by the transplant with a disadvantage for individuals transplanted from the entrance to the inner location. The here observed results may thus indicate that the disturbance gradient in marinas might constitute a staple for selecting pollutant-resistant species and populations, causing local adaptation. This highlights the importance of conducting further studies into small scale local adaptation.

Sexual isolation with and without ecological isolation in marine isopods Jaera albifrons and J. praehirsuta.

Sexual barriers associated with mate choice are often found to be associated with some level of ecological isolation between species. The independence and relative strength of sexual isolation are thus difficult to assess. Here, we take advantage of a pair of marine isopod species (Jaera albifrons and J. praehirsuta) that show sexual isolation and coexist in populations where they share the same microhabitat or not (i.e. without or with ecological isolation). We estimated the strength of sexual isolation between J. albifrons and J. praehirsuta using no-choice trials and a multiple-choice experimental population. We found that sexual isolation is strong in both the presence and the absence of ecological isolation, but that it is asymmetric and fails to prevent interspecific gene flow entirely. First-generation intrinsic post-zygotic barriers were low, and there was no sexual isolation within J. praehirsuta across habitats. The J. albifrons/J. praehirsuta species pair thus provides an example where the role of sexual isolation as a barrier to gene flow (a) does not depend upon current ecological isolation, (b) seems to have evolved independently of local ecological conditions, but (c) is insufficient to complete speciation entirely on its own.

Seascape Genomics of the Sugar Kelp Saccharina latissima along the North Eastern Atlantic Latitudinal Gradient.

Temperature is one of the most important range-limiting factors for many seaweeds. Driven by the recent climatic changes, rapid northward shifts of species' distribution ranges can potentially modify the phylogeographic signature of Last Glacial Maximum. We explored this question in detail in the cold-tolerant kelp species Saccharina latissima, using microsatellites and double digest restriction site-associated DNA sequencing ( ddRAD-seq) derived single nucleotide polymorphisms (SNPs) to analyze the genetic diversity and structure in 11 sites spanning the entire European Atlantic latitudinal range of this species. In addition, we checked for statistical correlation between genetic marker allele frequencies and three environmental proxies (sea surface temperature, salinity, and water turbidity). Our findings revealed that genetic diversity was significantly higher for the northernmost locality (Spitsbergen) compared to the southern ones (Northern Iberia), which we discuss in light of the current state of knowledge on phylogeography of S. latissima and the potential influence of the recent climatic changes on the population structure of this species. Seven SNPs and 12 microsatellite alleles were found to be significantly associated with at least one of the three environmental variables. We speculate on the putative adaptive functions of the genes associated with the outlier markers and the importance of these markers for successful conservation and aquaculture strategies for S. latissima in this age of rapid global change.

Genetic variability and transgenerational regulation of investment in sex in the monogonont rotifer Brachionus plicatilis.

In cyclical parthenogens such as aphids, cladocerans and rotifers, the coupling between sexual reproduction and the production of resting stages (diapausing eggs) imposes strong constraints on the timing of sex. Whereas induction of sex is generally triggered by environmental cues, the response to such cues may vary across individuals according to genetic and nongenetic factors. In this study, we explored genetic and epigenetic causes of variation for the propensity for sex using a collection of strains from a Spanish population of monogonont rotifers (Brachionus plicatilis) in which variation for the threshold population density at which sex is induced (mixis threshold) had been documented previously. Our results show significant variation for the mixis threshold among 20 clones maintained under controlled conditions for 15 asexual generations. The effect of the number of clonal generations since hatching of the diapausing egg on the mixis ratio (proportion of sexual offspring produced) was tested on 4 clones with contrasted mixis thresholds. The results show a negative correlation between the mixis threshold and mixis ratio, as well as a significant effect of the number of clonal generations since fertilization, sex being repressed during the first few generations after hatching of the diapausing egg.

Combined effects of global climate change and nutrient enrichment on the physiology of three temperate maerl species.

Made up of calcareous coralline algae, maerl beds play a major role as ecosystem engineers in coastal areas throughout the world. They undergo strong anthropogenic pressures, which may threaten their survival. The aim of this study was to gain insight into the future of maerl beds in the context of global and local changes. We examined the effects of rising temperatures (+3°C) and ocean acidification (-0.3 pH units) according to temperature and pH projections (i.e., the RCP 8.5 scenario), and nutrient (N and P) availability on three temperate maerl species (Lithothamnion corallioides, Phymatolithon calcareum, and Lithophyllum incrustans) in the laboratory in winter and summer conditions. Physiological rates of primary production, respiration, and calcification were measured on all three species in each treatment and season. The physiological response of maerl to global climate change was species-specific and influenced by seawater nutrient concentrations. Future temperature-pH scenario enhanced maximal gross primary production rates in P. calcareum in winter and in L. corallioides in both seasons. Nevertheless, both species suffered an impairment of light harvesting and photoprotective mechanisms in winter. Calcification rates at ambient light intensity were negatively affected by the future temperature-pH scenario in winter, with net dissolution observed in the dark in L. corallioides and P. calcareum under low nutrient concentrations. Nutrient enrichment avoided dissolution under future scenarios in winter and had a positive effect on L. incrustans calcification rate in the dark in summer. In winter conditions, maximal calcification rates were enhanced by the future temperature-pH scenario on the three species, but P. calcareum suffered inhibition at high irradiances. In summer conditions, the maximal calcification rate dropped in L. corallioides under the future global climate change scenario, with a potential negative impact on CaCO3 budget for maerl beds in the Bay of Brest where this species is dominant. Our results highlight how local changes in nutrient availability or irradiance levels impact the response of maerl species to global climate change and thus point out how it is important to consider other abiotic parameters in order to develop management policies capable to increase the resilience of maerl beds under the future global climate change scenario.

Impact of ocean acidification and warming on the productivity of a rock pool community.

This study examined experimentally the combined effect of ocean acidification and warming on the productivity of rock pool multi-specific assemblages, composed of coralline algae, fleshy algae, and grazers. Natural rock pool communities experience high environmental fluctuations. This may confer physiological advantage to rock pool communities when facing predicted acidification and warming. The effect of ocean acidification and warming have been assessed at both individual and assemblage level to examine the importance of species interactions in the response of assemblages. We hypothesized that rock pool assemblages have physiological advantage when facing predicted ocean acidification and warming. Species exhibited species-specific responses to increased temperature and pCO2. Increased temperature and pCO2 have no effect on assemblage photosynthesis, which was mostly influenced by fleshy algal primary production. The response of coralline algae to ocean acidification and warming depended on the season, which evidenced the importance of physiological adaptations to their environment in their response to climate change. We suggest that rock pool assemblages are relatively robust to changes in temperature and pCO2, in terms of primary production.

Geographically distinct patterns of reproductive isolation and hybridization in two sympatric species of the Jaera albifrons complex (marine isopods).

Sympatric species that in some populations hybridize and in other populations remain reproductively isolated open interesting research possibilities for the study of hybridization and speciation. Here, we test for such a situation in two littoral isopods (Jaera albifrons and J. praehirsuta) that occur in mixed populations and where past morphological descriptions suggested that the two species are generally reproductively isolated except in rare populations where hybridization may be happening. Using field surveys and microsatellite genetic structure analyses in two regions from France (Normandy and Brittany), we confirmed that introgressive hybridization occurs in a subset of mixed J. albifrons/J. praehirsuta populations (region Normandy) where the two species are found in the same habitat (pebbles on the shore). Moreover, we found that introgression in these populations is differential, 21 of 23 microsatellite markers showing little genetic divergence between species (hierarchical analysis of molecular variance FCT = 0.017) while the remaining two loci were strongly differentiated (FCT = 0.428). By contrast, J. albifrons and J. praehirsuta in mixed populations from region Brittany occupied distinct habitats (pebbles and seaweeds, respectively) with little overlap and showed stronger genetic divergence (FCT = 0.132). In hybridizing populations, the majority of individuals show morphological traits that are characteristic of one or the other species. This raises the question of the forces that act to maintain this polymorphism, noting that hybridizing populations seem to be geographically isolated from potential source parental populations and show no detectable habitat divergence between species.