RESUMO
RESUMO O artigo traz relatos de sonhos de pacientes em análise, ou não, durante a pandemia. Apresenta a interpretação de sonhos da pandemia de covid-19, segundo o modelo clássico da interpretação psicanalítica de sonhos, e destaca a simbolização como recurso desse tipo de interpretação.
ABSTRACT Dreams reports of patients being analyzed, or not, during the pandemic. Dreams interpretation of the covid-19 pandemic, according to the classic model of psychoanalytic dreams interpretation. Symbolization as a resource for psychoanalytic interpretation of dreams.
RESUMEN Informes de los sueños de los pacientes analizados o no durante la pandemia. Interpretación de los sueños de la pandemia de covid-19, según el modelo clásico de interpretación de los sueños psicoanalíticos. Simbolización, como recurso para la interpretación psicoanalítica de los sueños.
RÉSUMÉ Rapports de rêves de patients analysés ou non pendant la pandémie. Interprétation onirique de la pandémie de covid-19, selon le modèle classique de l'interprétation psychanalytique des rêves. Symbolisation comme ressource pour l'interprétation des rêves.
RESUMO
The distribution of 5-methylcytosine (5-MeC) was investigated in fish chromosomes by indirect immunofluorescence using a highly specific 5-MeC monoclonal antibody. Diploid and artificially produced triploid specimens of the pacu fish, Piaractus mesopotamicus, were analyzed. The strong immunofluorescent signals were coincident with the heterochromatic regions of both diploids and triploids in a pattern that matched the C-banding pattern. In the euchromatin, heterogeneous labeling was observed along the chromatids. The weakness of this labeling hindered comparison of the fluorescence labeling of homologous chromosomes from diploid and triploid individuals. However, no striking differences were observed. The possibility that the euchromatin labeling by the 5-MeC antibody is related to the occurrence of mildly repetitive sequences in the genome of Piaractus is discussed.
Assuntos
Mapeamento Cromossômico , Ciprinodontiformes/genética , Citosina/análogos & derivados , Metilação de DNA , 5-Metilcitosina , Animais , Citosina/fisiologia , Diploide , Dosagem de Genes , Hibridização in Situ Fluorescente , Metáfase/genética , PoliploidiaRESUMO
The methylation profile of ten alpha-satellites was investigated in normal individuals and in ICF (Immunodeficiency, Centromeric instability, Facial abnormalities) patients. Two out of three ICF patients showed modified methylation of these sequences, reproducing a placental profile. CENP-B boxes, the binding sites of centromeric protein B, were always skewed toward nonmethylation. Unexpected results were observed in normal individuals: in somatic adult tissues the methylation pattern of alpha-satellite DNA varied between chromosomes, and in fetal tissues these satellites were homogeneously undermethylated. Detailed methylation analysis of CENP-B boxes revealed that unmethylated alpha-satellite units coexist with thoroughly methylated regions. These observations showed that the two major components of constitutive heterochromatin are differently methylated in normal somatic and fetal tissues, since classical satellites are consistently methylated. The definite changes in the methylation profile of heterochromatin in somatic chromosomes and the asynchronous timing of methylation of classical and alpha-satellites during development may reflect specific roles of highly repeated sequences in genomic organization.