Field and laboratory characterization of rotenone attenuation in eight lakes of the Kenai Peninsula, Alaska.

Rotenone is a pesticide commonly used to eradicate Northern Pike (Esox lucius), an invasive species, in Southcentral Alaska. The present work incorporates a field investigation of rotenone attenuation in eight lakes of the Kenai Peninsula, following a CFT Legumine® treatment in October 2018 and a laboratory simulation to determine persistence under light/dark and sterile/nonsterile conditions representative of Southcentral Alaskan winters. In the field, rotenone degraded within <60 days of application in all lakes, while rotenolone, the primary product of rotenone degradation, persisted for up to <280 days post-treatment at two locations. Prolonged rotenolone attenuation was most likely caused by short days and ice cover between October and April. This hypothesis was supported by a laboratory simulation which revealed photolysis as the dominant process driving the overall degradation of rotenone and that microbial degradation will significantly contribute in the absence of sunlight under simulated "winter" conditions of 4 °C. Degradation model fit comparisons (pseudo-first order, multi-parameter linear, and gamma) indicate the most accurate prediction occurred when modeling all eight lakes grouped together in a single dataset, combined and treated with pseudo-first order model kinetics, based on Akaike information criteria (AIC) scores.

Identification of Rotenone and Five Rotenoids in CFT Legumine Piscicide Formulation via High Resolution Mass Spectrometry and a New High-Throughput Extraction Procedure.

The piscicide CFT Legumine is applied to freshwater systems around the world to control invasive fish species. Rotenone, a potent inhibitor of mitochondrial cellular respiration, is the active ingredient of the piscicide; however, other rotenoids of unknown persistence and toxicity account for an equivalent amount by weight. This work identified six distinct rotenoids in CFT Legumine using liquid chromatography coupled with high resolution orbitrap mass spectrometry and optimized a rapid surface water sampling procedure for their analysis. The rotenoids were identified as rotenone and its isomer deguelin, their 12α-hydroxylated products rotenolone and tephrosin, as well as 6α,12α-dehydrorotenone and 6α,12α-dehydrodeguelin. The optimized procedure, extraction with Spin-X nylon membrane microcentrifuge filters followed by elution with acetonitrile, achieved recoveries ranging from 101 - 107 % and 97 - 145 % for all six rotenoids at high (125 nM, ~50 ppb) and low (25 nM, ~10 ppb) concentrations of CFT Legumine, respectively. Overall, this method provides a rapid sampling procedure necessary for monitoring rotenoid persistence in surface water to ensure safe and efficacious application of the pesticide.

Emergence of complex behavior in chemical cells: the system AlCl3-NaOH.

Chemical cells that spontaneously form in simple inorganic systems are presented. The cells are surrounded by semipermeable membranes that allow water and some ions to diffuse through. These cells exhibit dynamical behaviors that are typically associated with biological entities. These behaviors may be used to perform tasks such as rotation or linear translation in the vertical and horizontal directions. Yet another system builds "curtains". Behaviors are controlled by a complex network of physical and chemical processes that are organized in space and time. The type of dynamical behavior is determined by the chemical composition of the cell and the environment. By studying these systems we may learn general rules for the growth of living entities, or at least about the spontaneous growth of complex chemical structures. Understanding and mastering the synthesis of these systems may lead to new technologies where complex structures are grown rather than assembled.