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Eur Cell Mater ; 35: 34-53, 2018 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-29424418

RESUMO

Although the composition and structure of cartilaginous tissues is complex, collagen II fibrils and aggrecan are the most abundant assemblies in both articular cartilage (AC) and the nucleus pulposus (NP) of the intervertebral disc (IVD). Whilst structural heterogeneity of intact aggrecan ( containing three globular domains) is well characterised, the extent of aggrecan fragmentation in healthy tissues is poorly defined. Using young, yet skeletally mature (18-30 months), bovine AC and NP tissues, it was shown that, whilst the ultrastructure of intact aggrecan was tissue-dependent, most molecules (AC: 95 %; NP: 99.5 %) were fragmented (lacking one or more globular domains). Fragments were significantly smaller and more structurally heterogeneous in the NP compared with the AC (molecular area; AC: 8543 nm2; NP: 4625 nm2; p < 0.0001). In contrast, fibrillar collagen appeared structurally intact and tissue-invariant. Molecular fragmentation is considered indicative of a pathology; however, these young, skeletally mature tissues were histologically and mechanically (reduced modulus: AC: ≈ 500 kPa; NP: ≈ 80 kPa) comparable to healthy tissues and devoid of notable gelatinase activity (compared with rat dermis). As aggrecan fragmentation was prevalent in neonatal bovine AC (99.5 % fragmented, molecular area: 5137 nm2) as compared with mature AC (95.0 % fragmented, molecular area: 8667 nm2), it was hypothesised that targeted proteolysis might be an adaptive process that modified aggrecan packing (as simulated computationally) and, hence, tissue charge density, mechanical properties and porosity. These observations provided a baseline against which pathological and/or age-related fragmentation of aggrecan could be assessed and suggested that new strategies might be required to engineer constructs that mimic the mechanical properties of native cartilaginous tissues.


Assuntos
Cartilagem Articular/metabolismo , Matriz Extracelular/metabolismo , Adsorção , Agrecanas/química , Agrecanas/metabolismo , Agrecanas/ultraestrutura , Sequência de Aminoácidos , Animais , Fenômenos Biomecânicos , Bovinos , Colágeno/metabolismo , Força Compressiva , Simulação por Computador , Gelatinases/metabolismo , Metaloproteinases da Matriz/metabolismo , Microscopia de Força Atômica , Nanopartículas , Núcleo Pulposo , Especificidade de Órgãos , Propriedades de Superfície
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