A Randomised Controlled Trial of a Universal Bonding Agent at Three Years: Self Etch vs Total Etch.

General dental practice is increasingly being recognised as the ideal situation for the conduct of clinical trials into the longevity of restorations. The aim of this study was to investigate the survival of 64 nanofilled resin composite (Filtek Supreme XTE) restorations placed principally in loadbearing cavities using a Universal dentine bonding agent (Scotchbond Universal), in five UK dental practices by members of the UK-based practice-based research group, the PREP Panel. A split mouth design was used, comprising patients who required two restorations, with one of the restorations receiving a total etch approach using phosphoric acid and the other being placed using a self-etch approach. The results indicated good performance of the restorations examined, with no difference, in terms of marginal characteristics, between the restorations which received total etching and those which did not.

A practice-based assessment of patients' knowledge of dental materials.

AIMS: It is the aim of this study to determine, by means of a questionnaire completed by patients attending ten UK dental practices, patients' level of knowledge on dental materials and techniques. MATERIALS AND METHODS: Members of The PREP (Product Research and Evaluation by Practitioners) Panel were asked to recruit patients to participate in a questionnaire-based assessment of their knowledge of dental materials. RESULTS: Two hundred and forty-nine patients took part in the questionnaire. Sixty-three percent (n = 157) of the respondents were female and 92% (n = 229) of the respondents stated they were regular attenders at the dental practice. The respondents were asked how important the quality of dental materials used in their mouth was, and on a Visual Analogue Scale (VAS) where 1 = not important and 10 = very important, the result was 9.6. The same score was recorded when they were asked how important it was that the materials used in their mouth were supported with relevant clinical research evidence and long term data of the success of the material. They were also questioned on the subjects of price, manufacturer, source or material and type of filling material. A significant amount of respondents demonstrated that they had concerns over the use of amalgam. CONCLUSIONS: Respondents expressed strong views that the materials used on their teeth should have a robust evidence base and they care about the materials that are used in their mouths.

How well does physician selection of microbiologic tests identify Clostridium difficile and other pathogens in paediatric diarrhoea? Insights using multiplex PCR-based detection.

The objective of this study was to compare the aetiologic yield of standard-of-care microbiologic testing ordered by physicians with that of a multiplex PCR platform. Stool specimens obtained from children and young adults with gastrointestinal illness were evaluated by standard laboratory methods and a developmental version of the FilmArray Gastrointestinal (GI) Diagnostic System (FilmArray GI Panel), a rapid multiplex PCR platform that detects 23 bacterial, viral and protozoal agents. Results were classified according to the microbiologic tests requested by the treating physician. A median of three (range 1-10) microbiologic tests were performed by the clinical laboratory during 378 unique diarrhoeal episodes. A potential aetiologic agent was identified in 46% of stool specimens by standard laboratory methods and in 65% of specimens tested using the FilmArray GI Panel (p < 0.001). For those patients who only had Clostridium difficile testing requested, an alternative pathogen was identified in 29% of cases with the FilmArray GI Panel. Notably, 11 (12%) cases of norovirus were identified among children who only had testing for Clostridium difficile ordered. Among those who had C. difficile testing ordered in combination with other tests, an additional pathogen was identified in 57% of stool specimens with the FilmArray GI Panel. For patients who had no C. difficile testing performed, the FilmArray GI Panel identified a pathogen in 63% of cases, including C. difficile in 8%. Physician-specified laboratory testing may miss important diarrhoeal pathogens. Additionally, standard laboratory testing is likely to underestimate co-infections with multiple infectious diarrhoeagenic agents.

Five-year clinical evaluation of zirconia-based bridges in patients in UK general dental practices.

OBJECTIVES: This study reported the results at 5 years of fixed-fixed all-ceramic bridges, constructed in a yttria oxide stabilized tetragonal zirconium oxide polycrystal (Y-TZP) substructure, placed in adult patients in UK general dental practices. MATERIALS AND METHODS: Four UK general dental practitioners recruited patients who required fixed bridgework and, after obtaining informed written consent, appropriate clinical and radiographic assessments were completed. The teeth were prepared and bridges constructed in accordance with the manufacturer's instructions. Each bridge was reviewed annually within 3 months of the anniversary of its placement by a calibrated examiner, together with the clinician who had placed the restoration, using modified USPHS criteria. RESULTS: Of the 41 bridges originally placed, 33 bridges were examined at 5 years. All Y-TZP frameworks were intact and no bridge retainers had debonded. Eight chipping fractures in the veneering ceramic were noted over the 5-year period. In five cases the patients were unaware of these and these cases were polished. Of the remaining three cases, in one a repair was attempted but was unsuccessful, but the bridge remained in satisfactory service. However, in the case involving a chipping fracture of the mesial-incisal angle of a central incisor, it was considered that replacement of the bridge was necessary. CONCLUSION: 97% (n=32) of the 33 Lava Y-TZP fixed-fixed bridges, evaluated in patients attending UK general dental practices, were found to be performing satisfactorily. CLINICAL RELEVANCE: The use of Y-TZP frameworks holds promise.

A clinical evaluation of all-ceramic bridges placed in patients attending UK general dental practices: three-year results.

OBJECTIVES: To report the results at year three of an evaluation of fixed-fixed all-ceramic bridges, constructed in a yttria oxide stabilized tetragonal zirconium oxide polycrystal (Y-TZP) substructure, placed in adult patients in UK general dental practices and luted using a self-adhesive resin-based cement. METHODS: Ethical approval was obtained. Four UK general dental practitioners were asked to recruit patients in accordance with the trial protocol. After obtaining informed written consent, appropriate vitality and radiographic assessments were completed and the pre-operative status of the gingival tissues noted. The teeth were prepared and bridges constructed in accordance with the manufacturer's instructions. Each bridge was reviewed annually within 3 months of the anniversary of its placement by a calibrated examiner, together with the clinician who had placed the restoration. The examiners evaluated the integrity of the restoration, its anatomic form, marginal adaptation, surface quality, sensitivity, the condition of the adjacent gingivae, and the presence or absence of secondary caries. RESULTS: A total of 34 bridges were examined at the three-year review. All Y-TZP frameworks were intact and no bridge retainers had debonded. Two veneering ceramic chips, in total, were detected over the three-year period of observation: the patients in whom this had occurred were unconcerned. A further abutment tooth had been successfully endodontically treated, through an occlusal access cavity, in addition to the two already reported at year one. SIGNIFICANCE: At year three, the 34 Lava Y-TZP fixed-fixed bridges, placed in patients attending UK general dental practices, were found to be performing satisfactorily.

A clinical evaluation of all-ceramic bridges placed in UK general dental practices: first-year results.

PURPOSE: To report the results at year one of a three-year evaluation of the performance of fixed all-ceramic bridges, constructed with a yttrium tetragonal zirconia polycrystal substructure placed in adult patients in UK general dental practices and cemented using a self-adhesive resin-based cement. METHODS: Ethical approval was obtained. Four UK general dental practitioners were asked to recruit patients complying with the trial criteria and protocol. After obtaining informed written consent, appropriate vitality and radiographic assessments were completed and the pre-operative status of the gingival tissues noted. The teeth were prepared and bridges constructed using the same technician and laboratory procedures. Each bridge was reviewed within three months of the anniversary of its placement by a calibrated examiner together with the clinician who had placed the restoration. The examiners evaluated the integrity of the restoration, its anatomic form, marginal adaptation, surface quality, sensitivity, the condition of the adjacent gingivae, and the presence or absence of secondary caries. RESULTS: All the bridges (n = 38) examined at the first-year review were present, intact and performing well, though one small chip of the veneering porcelain was detected and in two cases an abutment tooth had been endodontically treated through an occlusal access cavity.

Clinical performance of reinforced glass ionomer restorations placed in UK dental practices.

AIM: To retrospectively evaluate the performance of reinforced glass ionomer restorations placed in load-bearing surfaces of posterior teeth in three UK general dental practices. METHODS: Inclusion criteria for the participating practitioners were that they would be able to find, in their regularly attending patients' mouths, a minimum of 30 Fuji IX restorations placed in load-bearing cavities in posterior teeth. The three practitioners who agreed to participate were given training in the methods of assessment of restorations. Presence/absence of the restoration, presence of secondary caries, anatomic form, margin adaptation, margin discolouration, surface roughness and colour match were recorded. RESULTS: Three general dental practitioners and 169 restorations in 116 patients were included in the study. Seventy-eight percent of restorations were placed in molar teeth, the remainder in premolar teeth, with 67 being Class I and 102 Class II. The mean age of restorations at examination was 25 months, ranging from five months to 56 months. Of the restorations examined, 98% (n = 166) were found to be present and intact. No secondary caries was detected clinically. Three restorations were found to have fractured. CONCLUSION: Reinforced glass ionomer restorations placed in load-bearing situations in patients attending three dental practices in the UK were found to be performing satisfactorily at two years. Further investigations, of improved rigour, may now be indicated to more fully assess the performance of such restorations in the long term.

Two-year evaluation of restorations of a packable composite placed in UK general dental practices.

OBJECTIVE: The aim of this study was to assess the clinical performance at two years of 100 Solitaire 2 restorations placed in five United Kingdom dental practices by members of a practice-based research group. METHOD AND MATERIALS: Restorations were assessed after two years by a trained evaluator and the dental practitioner who had placed the material, for anatomic form, marginal adaptation, surface roughness, gingival condition and the presence or absence of secondary caries. In addition, the patients completed a questionnaire requesting details of the comfort and performance of the Solitaire 2 restoration(s). RESULTS: A total of 88 (58 Class II and 30 Class I) restorations of Solitaire 2 placed in 49 patients (mean age 43 years) were assessed. Twelve restorations could not be evaluated because of patient unavailability for the dates of the examinations. Two Class II restorations (2%) had failed by the time of the two-year evaluation and the remaining 86 restorations were found to be intact with no secondary caries. A high percentage of optimal scores were recorded for anatomic form and surface roughness. The colour match of two restorations (2%) was recorded as an obvious mismatch, but otherwise no unacceptable scores were recorded. CONCLUSIONS: After two years of clinical service a high proportion (96%) of the Solitaire 2 restorations that were available for re-examination, placed in general dental practice settings, were found to be performing satisfactorily.

A practice-based, randomized, controlled clinical trial of a new resin composite restorative: one-year results.

This study evaluated the performance of a low-shrinking resin composite compared with an amalgam for restoration of Class I and II cavities of moderate size in posterior teeth in a general practice setting. Fifty-two pairs of test and control restorations were placed in 49 patients. Clinical evaluations and assessments of replica models were carried out at baseline, six months and one year. Patients recorded their level of satisfaction with the restorations by means of visual analog scales. Apart from one control restoration that failed due to a fractured cusp, all of the restorations reviewed at six months and one year were intact with no unacceptable scores for any of the evaluation criteria. It was concluded that the resin composite evaluated, when used in conjunction with the recommended adhesive system, may be an appropriate alternative to amalgam in the restoration of posterior teeth over one year in clinical service.

Research in dental practice: a 'SWOT' analysis.

Most dental treatment, in most countries, is carried out in general dental practice. There is therefore a potential wealth of research material, although clinical evaluations have generally been carried out on hospital-based patients. Many types of research, such as clinical evaluations and assessments of new materials, may be appropriate to dental practice. Principal problems are that dental practices are established to treat patients efficiently and to provide an income for the staff of the practice. Time spent on research therefore cannot be used for patient treatment, so there are cost implications. Critics of practice-based research have commented on the lack of calibration of operative diagnoses and other variables; however, this variability is the stuff of dental practice, the real-world situation. Many of the difficulties in carrying out research in dental practice may be overcome. For the enlightened, it may be possible to turn observations based on the volume of treatment carried out in practice into robust, clinically related and relevant research projects based in the real world of dental practice.

Wastage of a silicone impression material in a general practice setting: a comparison between hand and automixing methods.

A practice-based, randomised, controlled trial has been undertaken to investigate the wastage of impression material when recording full arch one-stage, polyvinyl siloxane impressions using four techniques: putty and automix light viscosity material, putty and automix regular viscosity material, putty and tubed light viscosity material, and putty and tubed regular viscosity material. A total of 100 maxillary impressions, comprising 25 recorded using each of the four techniques, were obtained according to a predetermined scheme for randomisation. All material wasted in dispensing, mixing and applying each impression was determined by repeated weighing. Statistical comparisons of the mean weights of material wasted for each type of impression revealed that significantly (P < 0.01) less wastage occurred with the automix techniques. It is concluded that automix impression techniques may, in more extensive studies, be found to be more cost effective than tubed impression material techniques when recording impressions of the type investigated.

One-year retrospective clinical evaluation of hybrid composite restorations placed in United Kingdom general practices.

OBJECTIVE: The purpose of this study was to assess Pertac II restorations placed in general dental practice. METHOD AND MATERIALS: A total of 86 restorations (14 Class I, 10 Class II, 17 Class III, 5 Class IV, and 40 Class V) using a hybrid composite material (Pertac II) placed in 56 patients (mean age, 39 years) in 5 dental practices in the United Kingdom were assessed after 1 year by a trained evaluator and the dental practitioner who had placed the restoration. All the restorations were assessed for anatomic form, marginal adaptation, surface roughness, sensitivity or discomfort, gingival condition, and the presence or absence of secondary caries. RESULTS: Thirty-five (43%) restorations were placed in load-bearing situations, 9% of which were assessed as being in "heavy" occlusion. Five (6%) of the restorations were lost (all Class V restorations), 4 from premolars and 1 from a molar. Four were in wedge-shaped cavities and 1 small restoration was actually dislodged during examination. The remaining 81 (94%) restorations were found to be intact, with no secondary caries detected. A high percentage of optimal scores were recorded for both anatomic form and surface roughness. CONCLUSION: It is concluded that the evaluation demonstrated satisfactory clinical performance of Pertac II restorations after 1 year of clinical service in the wide variety of clinical situations seen in general dental practice. However, the potential for failure of hybrid composite materials in Class V situations appears high. It may be argued that clinicians who wish to use a resin-based material in a Class V situation should use a compomer or microfilled composite.

A practice-based assessment of the handling of a fast-setting polyvinyl siloxane impression material used with the dual-arch tray technique.

OBJECTIVE: The purpose of this study was to assess a new impression material used in conjunction with the dual-arch technique. METHOD AND MATERIALS: Ten members were selected at random from the Product Research and Evaluation by Practitioners (PREP) panel, a United Kingdom-based group of dental practitioners who are prepared to undertake research projects in their practices. Explanatory letters, a questionnaire, packs of the impression material, and dual-arch trays were distributed to the evaluators, together with instructions on the use of the materials. The practitioners were asked to use the materials and return the questionnaire after using the materials for a minimum of 10 impressions. RESULTS: Three evaluators had not previously used the dual-arch technique, six had used it in the past or occasionally, and one used the technique routinely. A total of 115 impressions were recorded with Quick Step. The overall rating for ease of removal of impressions from the mouth was 4.7 on a linear scale in which 5 represented easy to remove and 1 represented difficult to remove. Four of the evaluators rated the working time as excellent and the remaining six as good. Eight of the evaluators stated that the quality of fit of the single-unit restoration constructed with the combined-arch tray was the same as that obtained when a conventional tray technique was used. CONCLUSIONS: The impression material under test scored highly for application in circumstances where the fields above and below the preparation margin were dry or had limited moisture problems. Eighty percent of the evaluators considered the impression and dual-arch tray technique to be of benefit to their practice.

Multiple identities in Northern Ireland: hierarchical ordering in the representation of group membership.

A study was conducted to explore whether participants in Northern Ireland attend to, and process information about, different group members as a function of a single dimension of category membership (religion) or as a function of additional and/or alternative bases for group membership. Utilizing a bogus 'newspaper story' paradigm, we explored whether participants would differentially recall target attributes as a function of two dimensions of category membership. Findings from this recall measure suggested that information concerning ingroup and outgroup members was processed as an interactive function of both religion and gender intergroup dimensions. Religion was only used to guide processing of more specific information if the story character was also an outgroup member on the gender dimension. These findings suggest a complex pattern of intergroup representation in the processing of group-relevant information in the Northern Irish context.

Roles of the heparin and low density lipid receptor-related protein-binding sites of protease nexin 1 (PN1) in urokinase-PN1 complex catabolism. The PN1 heparin-binding site mediates complex retention and degradation but not cell surface binding or internalization.

We have previously described thrombin (Th)-protease nexin 1 (PN1) inhibitory complex binding to cell surface heparins and subsequent low density lipid receptor-related protein (LRP)-mediated internalization. Our present studies examine the catabolism of urinary plasminogen activator (uPA)-PN1 inhibitory complexes, which, unlike Th.PN1 complexes, bind almost exclusively through the uPA receptor. In addition, the binding site in PN1 required for the LRP-mediated internalization of Th.PN1 complexes is not required for the LRP-mediated internalization of uPA.PN1 complexes. Thus, the protease moiety of the complex partially determines the mechanistic route of entry. Because cell surface heparins are only minimally involved in the binding and internalization of uPA.PN1 complexes, we then predicted that complexes between uPA and the heparin binding-deficient PN1 variant, PN1(K7E), should be catabolized at the same rate as complexes formed with native PN1. Surprisingly, the uPA.PN1(K7E) complexes were degraded at only a fraction of the rate of native complexes. Internalization studies revealed that both uPA. PN1(K7E) and native uPA.PN1 complexes were initially internalized at the same rate, but uPA.PN1(K7E) complexes were rapidly retro-endocytosed in an intact form. By examining the pH dependence of complex binding in the range of 4.0-7.0, it was determined that the uPA.PN1 inhibitory complexes must specifically bind to endosomal heparins at pH 5.5 to be retained and sorted to lysosomes. These studies are the first to document a role for heparins in the catabolism of SERPIN-protease complexes at a point further in the pathway than cell surface binding, and this role may extend to other heparin-binding LRP-internalized ligands.

One-year clinical evaluation of compomer restorations placed in general practice.

OBJECTIVE: This study evaluated the in-service performance, after 1 year, of a sample of F2000 compomer restorations placed by 10 general dental practitioners as part of handling evaluation of this material. METHOD AND MATERIALS: Five general dental practitioners who participated in the handling evaluation were asked to cooperate in the 1-year assessment of the restorations. The practitioners were requested to contact patients and inquire if they were willing to attend at a specified time for examination of their restorations by a trained evaluator who would travel to the various practice locations. A total of 84 restorations were presented for examination. The mean age of the restorations at examination was 14 months. The restorations were assessed for anatomic form, marginal adaptation, surface roughness, sensitivity or discomfort, gingival condition, and presence or absence of secondary caries. RESULTS: Eighty-two restorations were intact. No secondary caries was detected. Optimal scores were obtained in a high proportion of the assessments. All patients reported satisfaction with the restorations, both in appearance and surface texture, and no symptoms were reported. CONCLUSION: The F2000 compomer restorations, placed in conjunction with its bonding system in general practice conditions, were found to be performing satisfactorily after 1 year.

A clinical evaluation of Class II composites placed using a decoupling technique.

PURPOSE: This study was undertaken to evaluate the three-year clinical performance of Class II restorations of a fine particle hybrid composite placed using a decoupling placement technique, whereby polymerization shrinkage stresses at the cervical tooth-restoration interface were minimized by separating the initial gingival increment of composite from the body of the restoration using a polyurethane varnish. MATERIALS AND METHODS: The study was a practice-based explanatory, single-center clinical trial. A minimum of 40 Class II restorations of the material under investigation (Tetric) was required to be placed in predominantly molar teeth in selected patients. The restorations were placed using a decoupling incremental technique, bonding being accomplished using Syntac and Heliobond. The restorations were reviewed within 1 month of placement, thereafter at 18 months, then at 2 and 3 to 4 years using predetermined codes and criteria. Impressions and photographic records were used to obtain retrospective, third-party assessments of the restorations. RESULTS: Of 43 restorations originally placed and found to have ideal characteristics at baseline review, 41 (95%) were reviewed at 18 months, 36 (84%) at two years, and 31 (74%) at 3 to 4 years. None of the restorations were found to have suffered significant deterioration, notwithstanding three restorations having been lost to the study because of the replacement of part of the restored tooth for causes other than the catastrophic failure of the material. No secondary caries or postoperative sensitivity was recorded. CONCLUSION: It was concluded that the findings support the use of the material under investigation in the restoration of moderate- to large-sized Class II preparations in selected patients. The decoupling placement technique employed may be found to be an effective means to limit postoperative sensitivity and to contribute to the favorable in-service performance of restorations of the type investigated. Good practice would now require a multicentered, randomized controlled trial to investigate the effectiveness of the material and, in particular, the decoupling technique under the conditions of everyday practice.

Treatment of dentinal hypersensitivity using a dentine bonding system.

AIMS AND OBJECTIVES: Many agents have been used in treatment of dentinal hypersensitivity in the past, but dentine bonding systems have recently been suggested as being effective. This study examined the effectiveness of a dentine bonding system in the treatment of dentinal hypersensitivity in dental practice conditions. METHODS: Dentists in two dental practices agreed to carry out the project. One practice was in the UK, the other in India. A total of 34 patients who were diagnosed to have dentinal hypersensitivity were treated using the dentine bonding system. Patients were requested to record their perception of their pain on a 100mm linear scale, pre-treatment, one day and one week post-treatment. RESULTS: All patients experienced relief of pain, both 1 day and 1 week after treatment. Profile plots of the patients' perceived pain scores for the two practices separately indicated that there was a general trend across both practices for these to fall quite sharply one day after treatment and then generally level out one week post-treatment. There was evidence indicating a possible difference in pain perception in the two communities from which the patients were drawn. CONCLUSION: The dentine bonding system evaluated was successful in reducing the pain of dentinal hypersensitivity, at least in the short term.