RESUMO
BACKGROUND: The identification of Pseudomonas aeruginosa (P. aeruginosa) isolates in sputum from cystic fibrosis (CF) patients can be challenging due to the multitude of phenotypic changes isolates undergo during adaptation to the microenvironment of the CF lung. METHODS: We report the occurrence of shared P. aeruginosa isolates which failed identification by phenotypic methodologies and required species specific polymerase chain reaction. P. aeruginosa isolates were genotyped by macrorestriction analysis. RESULTS: Analysis of atypical isolates revealed one clonal P. aeruginosa isolate and three smaller clusters. In contrast molecular typing of phenotypically characteristic P. aeruginosa isolates revealed only small clusters. Despite exhibiting higher levels of antimicrobial resistance, acquisition of atypical strains was not associated with significant changes in clinical decline. CONCLUSIONS: Our experience highlights the importance of accurate identification of bacterial isolates in CF lung disease to detect clonal spread of atypical isolates.
Assuntos
Fibrose Cística/microbiologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Técnicas de Tipagem Bacteriana , Fibrose Cística/fisiopatologia , DNA Bacteriano/genética , Eletroforese em Gel de Ágar , Eletroforese em Gel de Campo Pulsado , Genótipo , Humanos , Testes de Sensibilidade Microbiana , Fenótipo , Reação em Cadeia da Polimerase , Escarro/microbiologiaRESUMO
Pandoraea species are emerging opportunistic pathogens capable of causing chronic lung infections in cystic fibrosis patients. This study examined the interactions of 17 Pandoraea isolates from the five identified species (Pandoraea apista, Pandoraea norimbergensis, Pandoraea pulmonicula, Pandoraea sputorum and Pandoraea pnomenusa) plus two Pandoraea genomospecies isolates with lung epithelial cells and their ability to form biofilms in vitro. Only three isolates showed an ability to invade A549 lung epithelial cells, and only one isolate was able to form biofilms. In contrast, all isolates triggered a pronounced pro-inflammatory response, with elevation of both interleukin (IL)-6 (two- to 19-fold) and IL-8 (10- to 50-fold) above that observed for a control strain of Escherichia coli. This property is likely to be a major factor in the pathogenesis of the genus.
Assuntos
Betaproteobacteria/patogenicidade , Biofilmes/crescimento & desenvolvimento , Células Epiteliais/microbiologia , Pulmão/patologia , Virulência/genética , Betaproteobacteria/efeitos dos fármacos , Betaproteobacteria/imunologia , Betaproteobacteria/fisiologia , Linhagem CelularRESUMO
A case is presented of infective endocarditis caused by a previously undescribed Helcococcus-like organism, in a patient with human immunodeficiency virus infection. The organism, which could not be identified phenotypically in the routine laboratory, was identified using partial 16S rRNA gene sequencing.
