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1.
Front Microbiol ; 2: 259, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22203822

RESUMO

Potato mop-top pomovirus (PMTV) is one of a few viruses that can move systemically in plants in the absence of the capsid protein (CP). Pomoviruses encode the triple gene block genetic module of movement proteins (TGB 1, 2, and 3) and recent research suggests that PMTV RNA is transported either as ribonucleoprotein (RNP) complexes containing TGB1 or encapsidated in virions containing TGB1. Furthermore, there are different requirements for local or systemic (long-distance) movement. Research suggests that nucleolar passage of TGB1 may be important for the long-distance movement of both RNP and virions. Moreover, and uniquely, the long-distance movement of the CP-encoding RNA requires expression of both major and minor CP subunits and is inhibited when only the major CP sub unit is expressed. This paper reviews pomovirus research and presents a current model for RNA movement.

2.
J Gen Virol ; 90(Pt 12): 3051-3056, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19726609

RESUMO

For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on B. macrocarpa, but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP-RT.


Assuntos
Chenopodium quinoa/virologia , Clonagem Molecular , Folhas de Planta/virologia , Vírus de Plantas/patogenicidade , Vírus de RNA/patogenicidade , RNA Viral/genética , Beta vulgaris/virologia , Dados de Sequência Molecular , Doenças das Plantas/virologia , Vírus de Plantas/genética , Vírus de Plantas/crescimento & desenvolvimento , Vírus de RNA/genética , Vírus de RNA/crescimento & desenvolvimento , RNA Viral/metabolismo , Análise de Sequência de DNA
3.
J Gen Virol ; 90(Pt 3): 754-758, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19218222

RESUMO

Ten beet virus Q (BVQ) strains from six different countries were sequenced to characterize the readthrough (RT) domain of the coat protein (CP). The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are FM244643-FM244652. With three nucleotide additions of 5, 285 and 1 nt, the common RT of 76 kDa was found to be longer than the single reference available to date (35 kDa). It is hypothesized that multiple inoculation cycles on Chenopodium quinoa were responsible for these three deletions in the C-terminal part of the BVQ RNA-2 previously described. Two putative transmembrane domains, TM1 and TM2, were predicted in the consensus amino acid sequence of the ten BVQ strains, and the putative BVQ TM2 was aligned with that of potato mop-top virus.


Assuntos
Beta vulgaris/virologia , Proteínas do Capsídeo/química , Estrutura Terciária de Proteína/genética , Vírus de RNA/química , Análise de Sequência de DNA , Sequência de Aminoácidos , Proteínas do Capsídeo/genética , Chenopodium quinoa/virologia , Dados de Sequência Molecular , Vírus de RNA/genética , Alinhamento de Sequência , Deleção de Sequência
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