Genetic diversity analysis on cultivar and wild population of Rehmannia glutinosa based on ISSR markers / 中草药

Objective: To study the genetic diversity and genetic relationship of cultivar and wild population of Rehmannia glutinosa and its relative species by ISSR molecular marker technique, and provide the reference for R. glutinosa germplasm protection and breeding. Methods: 106 samples including cultivar and wild population of R. glutinosa and its relative species were studied by ISSR-PCR markers. Nei's genetic diversity index (H) and other parameters of genetic information were calculated by POPGEN 32, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software. Results: Seven ISSR primers generated 85 loci of which 83 loci were polymorphic. The percentage of polymorphie bands (PPB) of all samples was 97.65%. Nei's genetic diversity index (H) and Shannon's information index (I) were 0.2659 and 0.4125. The percentage of polymorphie bands (PPB) of cultivar of R. glutinosa was 30.59%. The percentage of polymorphie bands (PPB) of wild population of R.glutinosa in Henan province was 83.53%. In the cluster dendrogram, all samples were clustered into seven groups at the level of Genetic similarity coefficient (GS) 0.67. Conclusion: The results of ISSR analysis revealed that the level of genetic diversity between wild populations of R. glutinosa was higher than that within cultivar populations of R. glutinosa. The genetic diversity among wild populations of R. glutinosa in Henan province was higher than other region, which was consistent with authentic producing areas of R. glutinosa in this area. The relationships of wild population of R. glutinosa had no obvious correlation with their geographical distribution pattern.

Intrinsic link of asymmetric reflection and diffraction in non-Hermitian gratings.

Asymmetric reflection in Bragg gratings and asymmetric diffraction in diffraction gratings are both linked to parity-time (PT) symmetry in non-Hermitian optics, but their direct relation has not been examined. To fill this gap, we first consider a PT-symmetric sinusoidal grating to compare the contrast of forward and backward reflectivities and the ratio of ±1-order diffraction efficiencies. Analytical and numerical results show that they change with identical tendencies and peaks at same positions in a wide parameter space, indicating thus an intrinsic link in both PT symmetric and PT broken phases. The underlying physics is found to be that the unbalanced coupling strengths between forward and backward reflected waves are identical to those between 0-order and ±1-order diffracted waves. We then consider a non-Hermitian grating dynamically induced in cold atomic lattices to include higher-order diffractions and corresponding reflections.Full numerical calculations show that the aforementioned findings hold also true in this complicated but practical grating, even in more general non-Hermitian cases beyond the exact PT symmetry.

Coherent perfect absorption, transmission, and synthesis in a double-cavity optomechanical system.

We study a double-cavity optomechanical system in which a movable mirror with perfect reflection is inserted between two fixed mirrors with partial transmission. This optomechanical system is driven from both fixed end mirrors in a symmetric scheme by two strong coupling fields and two weak probe fields. We find that three interesting phenomena: coherent perfect absorption (CPA), coherent perfect transmission (CPT), and coherent perfect synthesis (CPS) can be attained within different parameter regimes. That is, we can make two input probe fields totally absorbed by the movable mirror without yielding any energy output from either end mirror (CPA); make an input probe field transmitted from one end mirror to the other end mirror without suffering any energy loss in the two cavities (CPT); make two input probe fields synthesized into one output probe field after undergoing either a perfect transmission or a perfect reflection (CPS). These interesting phenomena originate from the efficient hybrid coupling of optical and mechanical modes and may be all-optically controlled to realize novel photonic devices in quantum information networks.

Electromagnetically induced grating based on the giant Kerr nonlinearity controlled by spontaneously generated coherence.

We propose a scheme for realizing electromagnetically induced grating via the giant Kerr nonlinearity in a coherently driven four-level system with spontaneously generated coherence. In the presence of spontaneously generated coherence, Kerr nonlinearity can be enhanced with vanishing linear absorption. Thus, with a standing-wave coupling field, one can achieve a pure absorption grating, which leads the probe field to gather the zero-order direction when the detuning of the coupling field is on resonance. Moreover, we can obtain a pure phase grating, which diffracts a weak probe light into the first-order direction and the second-order direction when the detuning of the coupling field is off resonance.

Resonant gain suppression and superluminal group velocity in a multilevel system.

We investigate the interaction of an open (N + 1)-level extended V-type atomic system (i.e. a closed (N + 2)-level atomic system) with N coherent laser fields and one incoherent pumping field through both analytical and numerical calculations. Our results show that the system can exhibit multiple resonant gain suppressions via perfect quantum destructive interference, which is usually believed to be absent in closed three-level V system and its extended versions involving more atomic levels, with at most N - 1 transparency windows associated with very steep anomalous dispersions occurring in the system. The superluminal group velocity of the probe-laser pulse with at most N - 1 negative values can also be generated and controlled with little gain or absorption.

Dynamic generation of robust and controlled beating signals in an asymmetric procedure of light storage and retrieval.

We propose an efficient scheme for the robust and controlled generation of beating signals in a sample of stationary atoms driven into the tripod configuration. This scheme relies on an asymmetric procedure of light storage and retrieval where the two classical coupling fields have equal detunings in the storage stage but opposite detunings in the retrieval stage. A quantum probe field, incident upon such an atomic sample, is first transformed into two spin coherence wave-packets and then retrieved with two optical components characterized by different time-dependent phases. Therefore the retrieved quantum probe field exhibits a series of maxima and minima (beating signals) in intensity due to the alternative constructive and destructive interference. This interesting phenomenon involves in fact the coherent manipulation of two dark-state polaritons and may be explored to achieve the fast quantum limited measurement.

Steady optical spectra and light propagation dynamics in cold atomic samples with homogeneous or inhomogeneous densities.

We study both steady and dynamic optical responses of three samples with the same amounts of cold atoms but very different density functions. These samples are driven into the regime of electromagnetically induced transparency by a probe and a coupling in the Lambda configuration. When the coupling is in the traveling-wave pattern, all samples have the same transmission spectra and therefore identical transmitted pulses at the sample exits. In the case of a standing-wave coupling, however, very different reflection and transmission spectra are found for the three samples. Accordingly, reflected pulses at the sample entrances and transmitted pulses at the sample exits are quite sensitive to the spatial inhomogeneity of cold atoms. These interesting phenomena are qualitatively analyzed in terms of constructive and destructive interference between forward and backward probe photons scattered by a standing-wave atomic grating.

Double photonic bandgaps dynamically induced in a tripod system of cold atoms.

A tripod atomic system driven by two standing-wave fields (a coupling and a driving) is explored to generate tunable double photonic bandgaps in the regime of electromagnetically induced transparency. Both photonic bandgaps depend critically on frequency detunings, spatial periodicities, and initial phases of the two standing-wave fields. When the coupling and driving detunings are very close, a small fluctuation of one standing-wave field may demolish both photonic bandgaps. If the two detunings are greatly different, however, each standing-wave field determines only one photonic bandgap in a less sensitive way. Dynamic generation and elimination of a pair of photonic bandgaps shown here may be exploited toward the end of simultaneous manipulation of two weak light signals even at the single-photon level.

Dynamically induced double photonic bandgaps in the presence of spontaneously generated coherence.

We study a four-level double-Lambda system with spontaneously generated coherence driven by a standing-wave coupling field. It is found that two well-developed photonic bandgaps with reflectivities of about 90% can be generated on the probe resonance in the presence of maximal spontaneously generated coherence. The induced double photonic bandgaps become, however, severely malformed when spontaneously generated coherence vanishes. Dynamic control of the double photonic bandgaps may be exploited to achieve a novel two-port double-channel routing scheme for weak light signals in quantum networks.

Isolation and Characterization of a Moderately Halophilic Bacterium High-producing Esterase / 微生物学通报

A moderately halophilic bacterium strain DF-B6 capable of degrading Tween 20 was isolated from solar saltern brine by plate method.Based on the phenotype,physiological and biochemical character-istics,and the phylogenetic analysis of 16S rDNA sequence,strain DF-B6 was identified as Idiomarina.The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase,and not a li-pase.The maximum esterase activity was observed in 8% NaCl concentration at 50?C,pH 8.0.The effect of various divalent cations was studied on the activity of esterase from Idiomarina sp.DF-B6 at the concentra-tion of 10 mmol/L,Mg2+,Ca2+ and Mn2+enhanced the esterase activity,while Zn2+,Fe3+ and Cu2+ inhibited its activity.