RESUMO
Objective:To investigate the effectiveness of nasal endoscopic anterior lacrimal recess approach combined with temporary fenestration of the nasal septum in resecting recurrent nasal inverted papilloma. Methods:Patients with recurrent nasal inverted papilloma who underwent reoperation in our hospital during the past 2 years were included . The nasal septum may hinder full access to and effective treatment of the lesions at the anterior and medial wall of the maxillary sinus by endoscope, aspirator and surgical instrument in the narrow aperture of the prelacrimal recess, although these lesions could be observed by 70° nasal endoscope. Results:The nasal septum is temporarily opened on the basis of the prelacrimal recess approach, and the nasal endoscope and instrument was introduced through trans-septal window, so as to provide a better view of the operative field and the angular range of the instrument's movement. Conclusion:The recurrent nasal inverted papilloma could be successfully managed by re-endoscopic anterior lacrimal recess approach combined with temporary fenestration of the nasal septum, and no recurrence was observed during the 2-year follow-up. This surgical approach is recommended for the inverted papilla which originates from the anterior medial wall of the maxillary sinus, as the tumor can be removed completely using this surgical approach.
Assuntos
Aparelho Lacrimal , Papiloma Invertido , Humanos , Papiloma Invertido/cirurgia , Papiloma Invertido/patologia , Endoscopia , Seio Maxilar/cirurgia , Seio Maxilar/patologia , Aparelho Lacrimal/cirurgia , Resultado do Tratamento , Estudos RetrospectivosRESUMO
OBJECTIVE: To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity. METHOD: Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3ß (MAPLC3ß) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining. RESULT: CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3ß in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3ß in CNE2/DDP was lower than that in CNE2 cells (P<0. 05). CONCLUSION: Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.
Assuntos
Autofagia , Neoplasias Nasofaríngeas/genética , Raios X , Proteínas Reguladoras de Apoptose/genética , Proteína Beclina-1 , Carcinoma , Ciclo Celular , Linhagem Celular Tumoral/efeitos da radiação , Relação Dose-Resposta à Radiação , Humanos , Proteínas de Membrana/genética , Proteínas Associadas aos Microtúbulos/genética , Carcinoma Nasofaríngeo , Tolerância a RadiaçãoRESUMO
OBJECTIVE: To study the expression of cyclooxygenase-2 (COX-2) and matrix metalloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) and their relationship with lymph node metastasis. METHOD: The expression of COX-2 and MMP-2 in 86 NPC tissues and 30 normal nasopharyngeal tissues were evaluated by immunohistochemical analysis. RESULT: The positive rate of COX-2 and MMP-2 in NPC tissue was 75.58% and 66.28% respectively, which was significantly higher than that in normal nasopharyngeal (P < 0.01). The expression of both COX-2 and MMP-2 were positively correlated with lymph node metastasis (P < 0.01). There was a positive correlation between the expression of COX-2 and MMP-2 (P < 0.01). CONCLUSION: COX-2 and MMP-2 expression are increased in NPC, and they may cooperate in the course of lymph metastasis of NPC.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Ciclo-Oxigenase 2/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patologia , Adulto , Idoso , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Feminino , Humanos , Linfonodos/patologia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Prognóstico , Adulto JovemRESUMO
OBJECTIVE: To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and the carcinogenesis and invasive behavior of nasal and paranasal sinus carcinoma (NPSC). METHOD: The expression of COX-2 were detected in 69 case NPSC tissues, paracancerous atypical hyperplasia tissue and normal tissue by using immunohistochemical techniques of SP method. RESULT: The positive rate of COX-2 in NPSC tissues and atypical hyperplasia tissue and normal tissue was 65.22% and 42.31% and 3.33%, respectively. The positive rate of COX-2 in NPSC tissues was significantly higher than that in normal tissue (P < 0.01), and increased apparently with the lesion progressing from normal to atypical hyperplasia tissue to NPSC tissues, and also correlated with the increasing of tumor (P < 0.05). CONCLUSION: COX-2 may play an important role in the carcinogenesis and invasion of NPSC.
