Analysis of the Diagnostic Value of Transrectal Ultrasonography for Rectal Submucosal Lesions.

Objective: This study aims to investigate the diagnostic value of transrectal ultrasonography for rectal submucosal lesions. Methods: A retrospective analysis was conducted on 132 patients with rectal submucosal lesions admitted to our hospital from June 2018 to May 2022. All patients underwent colonoscopy, miniprobe endoscopic ultrasonography, and transrectal ultrasonography before surgery, obtaining definitive pathological results. The lesions displayed smooth morphological eminence of the mucosa under a colonoscope. Among the patients, there were 76 males and 56 females, with an average age of 50.6 years. Using pathology as the gold standard, the diagnostic accuracy of transrectal ultrasonography and miniprobe endoscopic ultrasonography for rectal submucosal lesions was calculated, and the difference between the two was compared using the chi-square (χ2) test. Results: The overall diagnostic accuracy of transrectal ultrasonography and miniprobe endoscopic ultrasonography for all rectal submucosal lesions was 95.5% and 74.2%, respectively. It was observed that transrectal ultrasonography was superior to miniprobe endoscopic ultrasonography, and the difference was statistically significant (χ2 = 25.48, P < .05). Conclusions: Transrectal ultrasonography demonstrates high diagnostic value for rectal submucosal lesions and may serve as the preferred choice for their examination.

Pseudotyped Viruses for Enterovirus.

Using a non-pathogenic pseudotyped virus as a surrogate for a wide-type virus in scientific research complies with the recent requirements for biosafety. Enterovirus (EV) contains many species of viruses, which are a type of nonenveloped virus. The preparation of its corresponding pseudotyped virus often needs customized construction compared to some enveloped viruses. This article describes the procedures and challenges in the construction of pseudotyped virus for enterovirus (pseudotyped enterovirus, EVpv) and also introduces the application of EVpv in basic virological research, serological monitoring, and the detection of neutralizing antibody (NtAb).

Context-Aware Block Net for Small Object Detection.

State-of-the-art object detectors usually progressively downsample the input image until it is represented by small feature maps, which loses the spatial information and compromises the representation of small objects. In this article, we propose a context-aware block net (CAB Net) to improve small object detection by building high-resolution and strong semantic feature maps. To internally enhance the representation capacity of feature maps with high spatial resolution, we delicately design the context-aware block (CAB). CAB exploits pyramidal dilated convolutions to incorporate multilevel contextual information without losing the original resolution of feature maps. Then, we assemble CAB to the end of the truncated backbone network (e.g., VGG16) with a relatively small downsampling factor (e.g., 8) and cast off all following layers. CAB Net can capture both basic visual patterns as well as semantical information of small objects, thus improving the performance of small object detection. Experiments conducted on the benchmark Tsinghua-Tencent 100K and the Airport dataset show that CAB Net outperforms other top-performing detectors by a large margin while keeping real-time speed, which demonstrates the effectiveness of CAB Net for small object detection.

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine.

Coxsackievirus A16 (CV-A16), one of major etiological agents of hand, foot and mouth disease (HFMD), causes outbreaks of the disease in young children all over the world. In order to promote the prevention and control of HFMD, the research and development of CV-A16 vaccine have been carried out in China. However, due to lacking of a recognized CV-A16 antigen detection method, the evaluation and quality control (QC) of vaccine effectiveness are greatly limited. In this study, we established a quantitative enzyme-linked immunosorbent assay (Q-ELISA) to determine the antigen concentration in CV-A16 vaccines that can be applied in manufacturing in China. A neutralizing antibody 16E1 was used as a capture antibody that can bind to various CV-A16 antigens of different subgenotypes, and an antiserum from CV-A16-immunized rabbit conjugated by HRP was suitable for detecting and quantifying CV-A16 antigens. The Q-ELISA was validated for specificity, linearity, accuracy, precision and robustness by using the CV-A16 antigen national standard (NS). Furthermore, we utilized the Q-ELISA to quantify antigen contents of vaccine bulks from six manufacturers and other intermediate products from one manufacturer. The results indicated that the Q-ELISA can satisfy the requirements of QC for all manufacturers involved.

Development of a pseudovirus-based assay for measuring neutralizing antibodies against Coxsackievirus A10.

Coxsackievirus A10 (CV-A10) has recently emerged as a major pathogen of hand, foot, and mouth disease in children worldwide. Currently no effective treatments are available; development of anti-CV-A10 vaccine is a most cost-effective way for CV-A10 prevention. Robust assay to measure neutralizing antibody (NtAb) titres elicited by vaccination would greatly prompt anti-CV-A10 vaccine development. Compare to the traditional neutralization assay based on inhibition of cytopathic effects (herein after referred to as cNT) which is time-consuming and labor-intensive, in this study we developed an efficient high-throughput neutralization antibody assay based on CV-A10 pseudoviruses (herein after referred to as pNT). In the pNT, anti-CV-A10 NtAb titre was negatively corresponded with the relative luminescent unit (RLU) produced by luciferase reporter gene incorporated in pseudovirus genome. As described in this study, the NtAb against CV-A10 could be detected within 10-16 h, anti- CV-A10 NtAb in 67 human serum samples were measured in parallel with pNT and cNT assays, a good correlation (r = 0.83,p < .0001) and good agreement(97%) were shown between cNT and pNT, indicating that the pNT provides a rapid and convenient procedure for measuring NtAb production against anti-CV-A10 NtAb measurement.

Propofol promotes apoptosis and suppresses the HOTAIR-mediated mTOR/p70S6K signaling pathway in melanoma cells.

Propofol is an intravenous anesthetic, which is widely used in clinical anesthesia induction and maintenance and is critical in the sedation of patients. However, the functions and mechanisms of propofol on apoptosis of melanoma cells remain unclear. The present study investigated whether propofol promotes cell apoptosis and suppresses the HOX transcript antisense RNA (HOTAIR)-mediated mechanistic target of rapamycin (mTOR) pathway in melanoma cells. B16F10 cells were cultured with different concentrations (0-10 µM) of propofol for 24 or 48 h. Proliferation and apoptosis of B16F10 cells were detected using MTT assay and flow cytometry. The pcDNA 3.1(-)-HOTAIR and pcDNA 3.1(-)-control plasmids were transfected into B16F10 cells using Lipofectamine 2000. In the present study, treatment with propofol significantly reduced viability, and induced apoptosis and caspase-3 activity in melanoma cells. Propofol treatment significantly inhibited HOTAIR expression and the expression of phosphorylated (p)-mTOR and p- p70S6K protein in melanoma cells. Overexpression of HOTAIR significantly increased viability of melanoma cells, and increased HOTAIR, p-mTOR and p-p70S6K protein expression in melanoma cells. These results indicated that propofol promotes apoptosis and suppresses the HOTAIR-mediated mTOR signaling pathway in melanoma cells.