Green synthetic natural carbon dots derived from Fuligo Plantae with inhibitory effect against alcoholic gastric ulcer.

Introduction: Fuligo Plantae (FP), the ash that sticks to the bottom of pots or chimneys after weeds burn, has long been used for its hemostatic effects and treatment of gastrointestinal bleeding. Nevertheless, the active ingredient of FP still needs to be further explored. Methods: The microstructure, optical and chemical properties of FP-CDs were characterized. An alcohol-induced gastric ulcer model was utilized to evaluate whether pre-administration of FP-CDs alleviated gastric bleeding symptoms and ameliorated gastric mucosal barrier disruption. In addition, the feces of each group of rats were extracted for 16S rDNA genome sequencing of intestinal flora. Results: FP-CDs with a diameter ranging from 1.4-3.2 nm had abundant chemical groups, which may be beneficial to the exertion of inherent activity. FP-CDs alleviated alcohol-induced gastric ulcer, as demonstrated by activating the extrinsic coagulation pathway, alleviating inflammation, and suppressing oxidative stress levels. More interestingly, FP-CDs can improve the diversity and dysbiosis of intestinal flora in rats with alcohol-induced gastric ulcer. Conclusion: These comes about illustrate the momentous inhibitory effects of FP-CDs on alcoholic gastric ulcer in rats, which give a modern methodology for investigating the effective ingredient of FP, and lay an experimental basis for the application of FP-CDs in the clinical treatment of alcoholic gastric ulcer.

Anxiolytic effects of Chrysanthemum morifolium Ramat Carbonisata-based carbon dots in mCPP-induced anxiety-like behavior in mice: a nature-inspired approach.

Introduction: Anxiety disorders have emerged as a predominant health concern, yet existing pharmacological treatments for anxiety still present various challenges. Chrysanthemum morifolium Ramat Carbonisata (CMRC) has been utilized in China for approximately 400 years as a therapeutic intervention for anxiety disorders. In this study, a novel type of carbon dots derived from the decoction of Chrysanthemum morifolium Ramat Carbonisata (CMRC-CDs) was identified and isolated, and their morphological structure and functional groups were characterized. Furthermore, the effects of CMRC-CDs on m-chlorophenylpiperazine (mCPP)-induced anxiety-like behaviour in mice were examined and quantified. In order to investigate the potential mechanisms of their anxiolytic effects, concentrations of hypothalamic-pituitary-adrenal (HPA) axis hormones, amino acid neurotransmitters, and monoamine neurotransmitters were measured. Methods: In this study, we synthesized CMRC-CDs and evaluated their potential anti-anxiety effects in a controlled experiment involving 48 male ICR mice. The mice were randomly divided into six groups, treated with CMRC-CDs at different doses for 14 days, and subjected to Open-Field (OF) and Elevated Plus Maze (EPM) tests. Post-behavioral evaluations, blood samples and brain tissues were collected for neurotransmitter and Hypothalamic-Pituitary-Adrenal (HPA) axis hormone quantification via ELISA. Additionally, cytotoxicity of CMRC-CDs was assessed using a Cell Counting Kit-8 (CCK-8) assay on RAW 264.7 cells. Results and Discussion: CMRC-CDs were spherical and homogeneously dispersed, with diameters ranging from 1.4 to 4.0 nm and an abundance of chemical groups on their surface. In the open-field (OF) test, mice pre-treated with CMRC-CDs demonstrated an increased proportion of time spent in the central area and a higher frequency of entries into the central area. In the elevated plus maze (EPM) test, mice pre-treated with CMRC-CDs exhibited a greater number of entries into the open arm and an extended duration spent in the open arm. CMRC-CDs were observed to decrease serum concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and corticosterone (CORT). Furthermore, CMRC-CDs were found to increase γ-aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) levels, while concurrently reducing glutamic acid (Glu) concentrations in brain tissue. CMRC-CDs demonstrated anxiolytic effects, which may be attributed to their modulation of hormones and neurotransmitters. This finding suggests the potential therapeutic value of CMRC-CDs in the clinical treatment of anxiety disorders.