[Progress in the clinical application of gene detection technology of deafness].

Along with the development of modern science and technology, the clinical application of deafness gene detection technology is becoming more and more extensive. Gene detection technology has experienced the process from the gene chip technology to the first generation DNA sequencing technology, and then to the second generation DNA sequencing technology. They are widely used in diagnosis of hereditary deafness etiology in clinical practice. In recent years, they are further applicated in the detection of neonatal deafness gene screening, prenatal diagnosis and pre marital and pre pregnancy. This paper will reviews the significance, technology progress, clinical diagnosis and application of gene detection technology.

[Adjacent rotated cutaneous flap to repair deep electrical burns at functional positions].

In this paper, five cases of electrical burns in 10 special areas were treated by adjacent rotated cutaneous flaps. The positions of injuries included 5 open joints injuries, 4 deep burns in heel region, 1 in perineum region. The results were satisfied except one case due to muscle necrosis and amputation was undertaken. This method is simple, effective, safe, reliable and suitable to repair injuries of any part of the body. It can shorten the course of disease and reduce the occurrence of complications.

[Establishment and characteristics of a human synovial sarcoma cell line].

A human synovial sarcoma cell line (HSS-84), from a painful mass excised locally near the left sternoclavicular joint, was established and has been continuously propagated in vitro for more than one year through 54 passages till the end of 1985. Doubling time was 65 hr. The index of mitosis was 28.8%. The chromosome number for most of the cells was hypertriploid. The cells, stored in liquid nitrogen, had a good revival. Cytologically, some cells, showing elongated, spindle, stellate or spidery shape with prominent processes, were arranged in loose or bundled pattern, similar to sarcoma cells; some cells showed ovoid and polygonal in mosaic arrangement, similar to epithelioid cells. Both features indicated biphasic differentiation of the cells. By electron microscopy, the cells consisted of light and dark cells with microvilli and obvious abnormality. Histopathologically, the tumor, from which HSS-84 originated, was a biphasic differentiation synovial sarcoma. It was composed of malignant spindle interstitial cells and malignant epithelioid cells lining the clefts or spaces. The latter was similar to cultured cells by electron microscopy. HSS-84 was identical with the donor. HSS-84 establishment will be useful for research on diagnosis, therapy, origin and biphasic differentiation of the synovial sarcoma.