RESUMO
Background: Heart failure (HF) is a syndrome with global clinical and socioeconomic burden worldwide owing to its poor prognosis. Jiashen Prescription (JSP), a traditional Chinese medicine (TCM) formula, exhibits unambiguous effects on treating HF. Previously, we have reported that underlying mechanisms of JSP by an untargeted metabolomics approach, but the contribution of gut microbiota and metabolic interaction to the cardioprotective efficacy of JSP remains to be elucidated. Materials and methods: Firstly, the rat model of heart failure was established by the permanent ligation of the left anterior descending coronary artery. The efficacy evaluation of JSP in treating HF rats was per-formed by left ventricular ejection fraction (LVEF). Then, 16S rRNA gene sequencing and LC/MS-based metabolomic analysis were utilized to explore the characteristics of cecal-contents microecology and plasma metabolic profile, respectively. After that, the correlation between intestinal micro-ecological characteristics and plasma metabolic characteristics was analyzed to explore the potential mechanism of the JSP treatment in HF. Results: JSP could improve the cardiac function of heart failure rats and thus ameliorate heart failure via enhancing rat LVEF. Results of intestinal flora analysis revealed that JSP not only adjusted gut microbiota disturbances by enriching species diversity, reducing the abundance of pathogenic bacteria (such as Allobaculum, Brevinema), as well as increasing the abundance of beneficial bacteria (such as Lactobacillus, Lachnospiraceae_NK4A136_group), but also improved metabolic disorders by reversing metabolite plasma levels to normality. Through the conjoint analysis of 8 metabolites and the OTUs relative abundance data in the 16srRNA sequencing results by WGCNA method, 215 floras significantly related to the eight compounds were identified. The results of the correlation analysis demonstrated a significant association between intestinal microbiota and plasma metabolic profile, especially the significant correlation of Ruminococcaceae_UCG-014 and Protoporphyrin IX, Ruminococcaceae_UCG-005, Christensenellaceae_R-7_group and nicotinamide, dihydrofolic acid. Conclusion: The present study illustrated the underlying mechanism of JSP to treat heart failure by affecting intestinal flora and plasma metabolites, provide a potential therapeutic strategy against heart failure.
RESUMO
Mycotoxins are a great potential threat to human health, and the progress in the development of mycotoxin detection methods is of an escalating importance with the increasing emphasis on food safety. Aptamer, performing the same function as antibody in specific binding with targets, exhibits profound potential in biosensing since its debut in 1990. Recent years have witnessed the rapid development of aptasensors for mycotoxin detection with the achievement of ultralow limit of detection and high sensitivity in the lab. However, there is still no officially approved aptasensing methods in mycotoxin detection application. In order to provide researchers with inspirations in the design and development of aptasensors for mycotoxin detection, we divide these aptasensors into two types, namely "on the surface" and "in the colloid", according to the location where the key sensing reaction occurs. We also systematically review aptasensors reported in the past 5 years under the abovementioned criterion of classification, and compare the advantages and disadvantages of each kind of aptasensors. Finally, we discuss prospective directions in the development of aptasensors for mycotoxin detection. This paper will offer insight and motivation to practitioners working on the research and practical application of aptasensors in the detection of mycotoxins and other substances.
