RESUMO
Sixteen new microsatellites were, identified by screening 7533 expressed sequence tags of Chinese mitten crab, Eriocheir sinensis from GenBank data we published. They were polymorphic with the PIC value ranged from 0.349 to 0.957, the number of alleles ranged from 22 to 48, and the observed and expected heterozygosities ranged from 0.375 to 1.000 and 0.366 to 0.983, respectively. Five loci could be applicable to genetic diversity and population structure of E. sinensis.
Assuntos
Alelos , Braquiúros/genética , Etiquetas de Sequências Expressas , Loci Gênicos , Repetições de Microssatélites , Polimorfismo Genético , AnimaisRESUMO
OBJECTIVE: In this study we observed the effect of air abrasion on enamel-composite resin adhesive strength in vitro. METHODS: Thirty teeth were prepared and divided into three groups. One group was air abraded and was acid-etched, another one was air abraded, and the other one was acid-etched. Next the bond and resin were used. Then we observed and compared the shear bond strength. The data were analysed by Chi square(chi(2)) test. RESULTS: Enamel-composite resin adhesive strength were not affected by acid etching and air abrading, but can be affected by air abrading alone. CONCLUSION: In clinic the traditional acidetch technique that used in composite resin restoration can be replaced with air abrasion surface preparation technique.