RESUMO
Antibody repertoire refers to the totality of the superbly diversified antibodies within an individual to cope with the vast array of possible pathogens. Despite this extreme diversity, antibodies of the same clonotype, namely public clones, have been discovered among individuals. Although some public clones could be explained by antibody convergence, public clones in naive repertoire or virus-neutralizing clones from not infected people were also discovered. All these findings indicated that public clones might not occur by random and they might exert essential functions. However, the frequencies and functions of public clones in a population have never been studied. Here, we integrated 2,449 Rep-seq datasets from 767 donors and discovered 5.07 million public clones - ~10% of the repertoire are public in population. We found 38 therapeutic clones out of 3,390 annotated public clones including anti-PD1 clones in healthy people. Moreover, we also revealed clones neutralizing SARS-CoV-2, Ebola, and HIV-1 viruses in healthy individuals. Our result demonstrated that these clones are predisposed in the human antibody repertoire and may exert critical functions during particular immunological stimuli and consequently benefit the donors. We also implemented RAPID - a Rep-seq Analysis Platform with Integrated Databases, which may serve as a useful tool for others in the field.
RESUMO
Objective@#To analyze the application effect of water circulation blanket combined with warming flushing fluid in patients with percutaneous nephrolithotomy.@*Methods@#A total of 86 patients with percutaneous nephrolithotomy who underwent surgery from January 2015 to August 2018 were enrolled in Gaochun People's Hospital of Nanjing. The prospective study was used and the patients were randomly divided into treatment group and control group for 43 patients in each group. The control group used conventional heat preservation measures and warming flushing fluid, and the treatment group used water circulation blanket combined with warming flushing fluid. The stress response, coagulation function, and adverse reactions were compared between the two groups.@*Results@#The body temperature, mean arterial pressure and heart rate of the treatment group 1 hour after surgery were (37.04±0.30) °C, (94.12±8.24) mmHg, (82.06±7.82) times/min, respectively, after the completion of the operation, the indexes were (36.97±0.40) °C, (95.36±8.15) mmHg, and (82.78±8.21) times/min. The control group 1 hour after surgery indexes was (36.74±0.36) °C, (90.12±8.21) mmHg, (85.78±8.32) times/min, after the completion of the operation, the indexes were (35.76±0.42) °C, (90.42±6.54) mmHg, and (90.32±7.43) times/min. The difference between the two groups was statistically significant (t=2.136-13.680, P<0.05 or 0.01). The serum fibrinogen, activated partial thrombin time and thrombin time in the treatment group were (3.10±0.52) g/L, (30.08±1.85) s, (17.02±1.60) s, respectively, and the control group was (3.85±0.60) g/L, (33.42±1.74) s, (19.56±1.75) s. The difference between the two groups was statistically significant (t=6.194, 8.624, 7.024, P<0.05 or 0.01).The incidence of adverse reactions such as hypothermia in the treatment group was 6.98% (3/43), which was significantly lower than that in the control group 27.91% (12/43). The difference was statistically significant (χ2=6.541, P<0.05).@*Conclusions@#The water circulation blanket combined with warming flushing fluid can help relieve the stress response of patients with percutaneous nephrolithotomy, maintain the body's coagulation function stable, and reduce adverse reactions such as intraoperative hypothermia.
RESUMO
Objective To analyze the application effect of water circulation blanket combined with warming flushing fluid in patients with percutaneous nephrolithotomy. Methods A total of 86 patients with percutaneous nephrolithotomy who underwent surgery from January 2015 to August 2018 were enrolled in Gaochun People's Hospital of Nanjing. The prospective study was used and the patients were randomly divided into treatment group and control group for 43 patients in each group. The control group used conventional heat preservation measures and warming flushing fluid, and the treatment group used water circulation blanket combined with warming flushing fluid. The stress response, coagulation function, and adverse reactions were compared between the two groups. Results The body temperature, mean arterial pressure and heart rate of the treatment group 1 hour after surgery were (37.04±0.30)°C, (94.12± 8.24) mmHg, (82.06±7.82) times/min, respectively, after the completion of the operation, the indexes were (36.97±0.40)°C, (95.36±8.15) mmHg, and (82.78±8.21) times/min. The control group 1 hour after surgery indexes was (36.74±0.36) °C, (90.12±8.21) mmHg, (85.78±8.32) times/min, after the completion of the operation, the indexes were (35.76 ± 0.42) °C, (90.42 ± 6.54) mmHg, and (90.32 ± 7.43) times/min. The difference between the two groups was statistically significant (t=2.136-13.680, P<0.05 or 0.01). The serum fibrinogen, activated partial thrombin time and thrombin time in the treatment group were (3.10 ± 0.52) g/L, (30.08±1.85) s, (17.02±1.60) s, respectively, and the control group was (3.85±0.60) g/L, (33.42±1.74) s, (19.56± 1.75) s. The difference between the two groups was statistically significant (t=6.194, 8.624, 7.024, P<0.05 or 0.01).The incidence of adverse reactions such as hypothermia in the treatment group was 6.98% (3/43), which was significantly lower than that in the control group 27.91% (12/43). The difference was statistically significant (χ2=6.541, P<0.05). Conclusions The water circulation blanket combined with warming flushing fluid can help relieve the stress response of patients with percutaneous nephrolithotomy, maintain the body's coagulation function stable, and reduce adverse reactions such as intraoperative hypothermia.
