RESUMO
Functional recognition imprints of virus-like particles, at the surface of silica particles, were generated following a strategy based on protein-templated polycondensation of organosilanes.
Assuntos
Nanoestruturas/química , Vírus/metabolismo , Ácido Cítrico/química , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Impressão Molecular , Nanopartículas/química , Norovirus/química , Norovirus/metabolismo , Dióxido de Silício/química , Vírus/químicaRESUMO
Major stumbling blocks in the production of fully synthetic materials designed to feature virus recognition properties are that the target is large and its self-assembled architecture is fragile. Here we describe a synthetic strategy to produce organic/inorganic nanoparticulate hybrids that recognize non-enveloped icosahedral viruses in water at concentrations down to the picomolar range. We demonstrate that these systems bind a virus that, in turn, acts as a template during the nanomaterial synthesis. These virus imprinted particles then display remarkable selectivity and affinity. The reported method, which is based on surface imprinting using silica nanoparticles that act as a carrier material and organosilanes serving as biomimetic building blocks, goes beyond simple shape imprinting. We demonstrate the formation of a chemical imprint, comparable to the formation of biosilica, due to the template effect of the virion surface on the synthesis of the recognition material.
Assuntos
Impressão Molecular/métodos , Nanoestruturas/química , Vírus/metabolismo , Ligação Competitiva , Coloides , Cinética , Nanoestruturas/ultraestrutura , Dióxido de Silício/química , Propriedades de Superfície , Tombusvirus/química , Tombusvirus/ultraestrutura , Tymovirus/química , Tymovirus/ultraestrutura , Vírion/química , Vírion/ultraestrutura , Vírus/ultraestruturaRESUMO
An amphiphilic calix[4]arene derivative bearing four guanidino moieties at the upper rim and four dodecyl chains at the lower rim was shown to form stable solid lipid nanoparticles (SLNs) in water. The study of the interactions of these cationic SLNs with DNA revealed a sequence-dependent groove binding mechanism.
Assuntos
Calixarenos/química , DNA/química , DNA/genética , Lipídeos/química , Nanopartículas/química , Fenóis/química , Sequência de Bases , Eletricidade EstáticaRESUMO
The interactions of silver nanoparticles capped by various calix[n]arenes bearing sulphonate groups at the para and/or phenolic faces with cationic, neutral and anionic surfactants have been studied. Changes in the plasmonic absorption show that only the calix[4]arene derivatives sulphonated at the para-position interact and then only with cationic surfactants. The interactions follow the CMC values of the surfactants either as simple molecules or mixed micelles.
RESUMO
Polycationic calixarene-based solid lipid nanoparticles (SLNs) have been loaded at their surface with DNA and chitosan and studied for their ability to transfect mammalian cells.
