Age influence on periodontal tissues: a histological study.

Oral mucosa becomes thinner, smooth and looses stippling aspect with aging. From histological standpoint appears: narrowing and alteration of gingival epithelium, modification of epithelial-connective interface and decreasing of keratinization. However, it cannot be detected significant histological alterations in size, shape or arrangement of epithelial cells that could be endorsed to aging process. Histological studies indicate: decreasing of keratinization, regressive changes in epithelium and fibrosis in underlying connective tissue. Parakeratosis is frequent with aging because of microtraumas, in many cases is expression of permanent inflammation.

Indirect pulp capping in young patients: immunohistological study of pulp-dentin complex.

Indirect capping is a complex therapy exclusively needed in deep cavities that provides, using biomaterials, a disinfection of the dentinary sore and seals the dentinary tubules, protects the pulp of physical mechanisms and chemical agents and stimulates the mechanisms that produce new dentin. Following this idea, we studied the histological changes in the dental pulp tissue and also the specific immunohistochemical response in various structures when an indirect capping technique was used. We used special histological techniques followed by classical staining or by immunohistochemical reaction in order to assess the odontoblastic, and the vascular reaction. The immunohistochemical study allows us to evaluate the changes in the pulp-dentin complex, as the result of the changes in the dentinal tubules permeability and the biological reactions at this level.