Trace Metals in the Freshwater Fish Cyprinus carpio: Effect to Serum Biochemistry and Oxidative Status Markers.

Interactions between trace metals, serum biochemical parameters, and oxidative status markers were observed. Freshwater fish Cyprinuscarpio blood samples (n = 38) were collected at the beginning of May (n = 19) and at the end of July (n = 19) of 2015. The concentrations of metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Se, Sr, and Zn) were analyzed in blood serum samples of fishes by inductively coupled plasma optical emission spectrometry (ICP-OES), and Hg was determined by cold-vapor atomic absorption spectroscopy (CV-AAS). The general scheme of descending concentrations of metals in blood serum samples was as follows: Zn > Fe > Cu > Sr > Cr > Ni > Mn > Pb > Se > As > Cd > Hg. Zn was the most accumulated element (4.42-119.64 mg/L) in both seasons. Overall, the trace element content was higher in spring season, except Hg, Ni, Se, and Sr. The seasonal effect was confirmed for Mn, Zn, Mg, Glu, AST, and Chol levels and for most oxidative status markers. The gender effect was confirmed for Sr, GPx, PC, Chol, and CK concentrations. Trace metals (especially Cd, Cr, Cu, Fe, Hg, Mn, Ni, Sr, Zn, As) significantly affected some blood serum chemistry parameters. The correlation analysis between oxidative status markers (ROS, TAC, MDA, SOD, GSH, UA, BHB, and Alb) and trace metal (Cd, Cu, Ni, Sr, Hg, Pb, Fe, Mn) content confirmed statistically significant interactions in both seasons. Obtained results indicate specific actions of trace metals.

Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters.

The main objective of this study was to assess the concentration of various heavy metals (Cd, Pb, Zn, Cu, Hg) in the blood of sheep, followed by biochemical and hematological analysis in order to reveal possible associations. Blood was collected in two different seasons: winter (fed by fodder) and spring (grazing animals). The higher concentrations of Pb (p < 0.01), Cu (p < 0.05) and Hg, but lower of Cd and Zn were found in spring. Evaluation of the biochemical and hematological parameters during different seasons showed a possible environmental effect on the health of animals. A statistically significant increase of Ca (p < 0.001), Mg (p < 0.05), urea (p < 0.001), TP (p < 0.05), glucose (p < 0.01), AST (p < 0.001), ALT (p < 0.001), ALP (p < 0.01), cholesterol (p < 0.001), bilirubin (p < 0.05), triglycerides (p < 0.001) and a decrease of P (p < 0.05), HGB (p < 0.05), MCHC (p < 0.05) and RDWc (p < 0.05) in spring was detected. The results of this study showed statistically significant correlations between Pb and ALP (r = 0.53) level in winter and between Pb and Ca (r = -0.73) in the spring. The hematological analysis revealed a significant correlation between Zn and RBC (r = 0.61), MCV (r = -0.74), MCH (r = -0.71) and between Pb and MCH (r = -0.55), PCT (r = -0.66), PDWC (r = -0.55) in the winter. A high positive significant correlations were found between Cd and RDWC (r = 0.77) and Cu and RDWC (r = 0.75). The significance of this work is the use the data in the preventive diagnosis of metabolic and production diseases. The collected data may serve as a control indicator to detect toxic hazards related to the heavy metal occurrence on animal health status.

Blood concentration of copper, cadmium, zinc and lead in horses and its relation to hematological and biochemical parameters.

Environmental pollution results in serious health hazards to animals and blood analysis serves as a good alternative for health status assessment. The target of this study was to analyze the concentration of selected metals in equine blood, to analyze the blood parameters and to find possible correlations. Blood samples were collected from the vena jugularis of healthy adult horses. The highest concentration of all elements was found in whole blood (Cu 3.84 ± 0.90 mg L(-1); Cd = 0.81 ± 0.90 mg L(-1); Zn 26.67 ± 14.12 mg L(-1); Pb 9.33 ± 5.76 mg L(-1)). Higher concentrations of copper, cadmium, zinc and lead were detected in blood clots compared to blood sera (44.04%). A similar tendency was found for cadmium (50%), zinc (13.08%) and lead (46.02%), which showed generally higher concentrations in blood clots (cells). Correlation analysis proved some relations between analyzed elements. In blood clots there is a strong positive correlation between Cd - Pb (r = 0.93) and Zn - Pb (r = 0.71) was detected. For biochemical and hematological parameters mainly medium correlations were detected. Obtained results prove different correlations of analyzed elements in blood components as well as the effect on parameters of blood biochemical and hematological profiles.