RESUMO
El cuestionario que se presenta ha sido desarrollado y validado por miembros del Grupo de Innovación Docente Consolidado (GIDC) Grup d'Infermeria Orientat a Tècniques Educatives Innova-dores (GIOTEI) reconocido por el Programa de Millora i Innovació Docent (PMID) de la Universitat de Barcelona (UB).El propósito del estudio es aportar la descripción y resultados de un proceso de validación del instrumento GIOTEI-UB, creado para medir la competencia transversal trabajo en equipo en la formación de grado de Enfermería. Las pruebas psicométricas que se presen-tan comportan una segunda ronda de validación. Se trata de un cuestionario integrado por 39 ítems distribuidos en seis dimensiones y subescalas que valoran la participación en el trabajo en equipo desde la percepción de los estudiantes, a partir de la realización de actividades y de las habilidades interpersonales. Ha sido suministrado a estudiantes de los cuatro cursos del grado de Enfermería en el curso académico 2015-2016, en modalidad online a través del campus virtual de la UB. Las respuestas obtenidas son n=450 a través de la evaluación por pares y n=344 a través de autoevaluación. En general, los resultados muestran la validez y fiabilidad del cuestionario. Su aplicación ha mostrado el potencial evaluativo y también formativo del instrumento, plausible de ser contemplado para la docencia y la investigación en educación superior
The questionnaire presented was developed and validated by members of a Consolidated Teaching Innovation Group (GIDC) Grup d'Infermeria Orientat a Tècniques Educatives Innovadores (GIOTEI) recognized by the Millora i Innovació Do-cent Program (PMID) of the University of Barcelona (UB) The purpose of the study is to provide description and results of a second round of a validation process of the GIOTEI instrument, created to measure the transversal competency of teamwork in nursing degree training. It is a questionnaire composed of 39 items distributed in six dimensions or subscales that evaluate the participation in teamwork based on the realization of activities and interpersonal skills. It has been provided to students of the four classes of the Nursing degree in the academic year 2015-2016, in online mode through the virtual campus of the University of Barcelona. The answers obtained were n=450 through peer evaluation and n=344 through self-evaluation. In general, the results show good validity and reliability of the questionnaire. The application has shown the evaluative and formative potential of the instrument, eligible for consideration for teaching and research in higher education
Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Inquéritos e Questionários , Estudantes de Enfermagem , Equipe de Enfermagem , Autoavaliação (Psicologia) , Estudos Transversais , Revisão por Pares , Análise por Pareamento , PsicometriaRESUMO
OBJECTIVE: Methylation of a CpG island within the Methylation controlled DNAJ (MCJ) gene results in loss of expression in normal and neoplastic cells. Normal ovarian surface epithelial cells are methylated at the MCJ CpG island and do not express the MCJ gene. Furthermore, re-expression of the MCJ gene, in ovarian cancer cell lines, has been correlated with increased sensitivity to several important chemotherapeutic drugs. The objective of this study was to determine the extent of MCJ promoter methylation in epithelial ovarian cancer patients and address the possible role of MCJ methylation levels in response to chemotherapy in ovarian cancer patients. METHODS: The methylation status of 35 CpG sites within the MCJ CpG island was determined by sequencing of sodium bisulfite modified tumor DNA in 41 patients with stage III/IV epithelial ovarian tumors. Levels of methylation of the MCJ CpG island were then compared with response to therapy and overall survival in the patients. RESULTS: The analysis identified frequent loss of MCJ methylation in ovarian tumors, with only a subset retaining high methylation levels. While 93% (38/41) of tumors examined showed some level of MCJ methylation, only 17% (7/41) retained very high levels (>90% methylation). The presence of such high levels of CpG island methylation correlated significantly with poor response of patients' tumors to therapy (P = 0.027) and poor overall survival (P = 0.023, hazard ratio = 2.9). CONCLUSIONS: These results suggest that MCJ methylation may be useful as a marker of response to chemotherapy in ovarian cancer and are consistent with previous in vitro data linking loss of MCJ expression with drug resistance.
