Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers.

A molecular approach, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.