Efficacy of the Therapeutic Game "Trisquel" in the Treatment of Patients With Substance-Related Disorders Randomized Clinical Study.

Substance-related disorders (SRD) have been consistently associated with alterations both in cognitive and executive functions, which affect to patients' quality of life. The main objective of this work was to test the beneficial cognitive effects on patients with SRD after the implementation of "Trisquel," an intervention program in board game format. To check the effectiveness of Trisquel program, a group of people diagnosed with SRD was randomly assigned either to the experimental group or to the control group. The experimental group performed Trisquel structured sessions twice a week during 3 months, while the control group performed routinely conventional therapeutic activities with the same frequency and duration. Neuropsychological tests were done to both groups before and after the intervention. After the 3 months of intervention the experimental group showed the following statistically significant improvements for WAIS-III subtests: number key, symbol search, arithmetic, direct digits, inverse digits, total digits, letters-numbers in the processing speed index and in the working memory index. Regarding STROOP tests, statistically significant progress was observed in the phonetic fluency letter P, phonetic fluency letter M, phonetic fluency letter R subtests, word-reading and word-color subtests. The control group only obtained improvements for WAIS-III subtests of arithmetic, letters-numbers and in the working memory index. The results of this study confirm that "Trisquel" is an effective intervention program for people diagnosed with SRD, getting improvements in processing speed (psychomotor and reading), attentional subprocesses (focused and sustained) and executive functions (updating and inhibition).

Reduction of Shiga toxin-producing Escherichia coli in a beef abattoir.

The aim of this work was to reinforce actions tending to reduce Shiga toxin-producing Escherichia coli (STEC) in beef products from an Argentinean commercial abattoir implementing Hazard Analysis and Critical Control Point (HACCP) practices. An environmental stx map was built with 421 environmental samples from the slaughter, quartering, cool chamber and deboning sectors (February-May 2013). For stx determination, 125 carcass and 572 anatomical cut samples were used. Based on the environmental stx mapping results, improvement actions were designed and implemented (June and July 2013). After implementing improvement actions, 160 carcass and 477 anatomical cut samples were collected to identify stx and verify the impact of improvement actions (August-December 2013). Our results showed stx-positivity in pre-operational (10.1%) and operational (15.5%) environmental samples and in carcass and beef cut samples before (4.8 and 10.1%; p = 0.144) and after (1.2 and 4.8%; p = 0.0448) implementing improvement actions, respectively. Although improvement actions reduced stx in beef cuts, it is difficult to implement and sustain a system based on stx zero-tolerance only by reinforcing Good Manufacturing Practices, Sanitation Standard Operating Procedures and HACCP practices. The application of combined intervention strategies to reduce STEC in carcasses and beef cuts should be therefore considered.

Evolution of Nursing Workload Indicators Since the Implementation of the Electronic Health Record at a Tertiary Hospital in Spain.

Nurses can be overwhelmed by the growing need for documentation derived from the implantation of electronic health records. The objective was to describe the evolution of nursing workload since the implementation of the EHR. We performed a longitudinal study of global workload indicators over a 5-year period at a referral hospital in Spain since introduction of the EHR (2014). Clinical activity records of each nurse were monitored using audit logs of their accesses to EHRs. During the study period, the number of EHR sessions, the number of EHR sessions in which a nursing order was changed, and the time needed to complete each session significantly increased. The number of mouse clicks and keystrokes and the time required to complete each nursing order decreased. Documentation of the following nursing tasks increased: administration of medication, peripheral vascular catheters, urinary catheters, pressure ulcers, nursing assessment forms, and pre-surgical verification. In conclusion, since the implementation of the EHR, an increase in the workload of nursing professionals-estimated through indirect indicators-has been observed due to greater documentation.

Effect of slicing and storage temperatures on biochemical aspects of membrane integrity in two different genotypes of tomato.

BACKGROUND: Fresh-cut vegetables are subjected to multiple stressing agents including: (i) slicing, which induces cellular decompartmentalization; (ii) low refrigeration temperatures, responsible for chilling injury in the most sensitive products (e.g. tomatoes), and (iii) storage time because tissue senescence and aging can occur and reduce the shelf-life. In tomato slices, one of the most important issues is the membrane, which is responsible for several disorders related to the alteration of physiological processes, including ethylene biosynthesis. RESULTS: Electrolyte leakage and the content of thiobarbituric acid reactive substances in sliced tomatoes increased over time at two storage temperatures (4 °C and 15 °C) compared with intact fruit for the commercial variety (cultivar) Jama used as reference. However, in the tomato Italian landrace Canestrino, electrolyte leakage in sliced fruits increased after 120 h of storage compared to intact tomatoes, while the thiobarbituric acid reactive substance content increased rapidly over time at both storage temperatures. In the packages, higher ethylene content and carbon dioxide concentrations were detected in sliced tomatoes compared with intact fruits for both genotypes. In the most sensitive genotype for slicing (Jama), phospholipase C activity increased in tomato slices after 24 h of storage, but phospholipase D reached a higher value only at 168 h after processing at 4 °C of storage. CONCLUSIONS: The results evidence that the main damage in slices of full ripe tomatoes is more related to cutting, rather than chilling injury due to storage temperatures, with differences related to the genotype. Slicing enhanced membrane catabolism, ethylene production, and enzyme activity of phospholipases with a significant genotype effect. © 2021 Society of Chemical Industry.

Mineralocorticoid Receptor in Myeloid Cells Mediates Angiotensin II-Induced Vascular Dysfunction in Female Mice.

Enhanced mineralocorticoid receptor (MR) signaling is critical to the development of endothelial dysfunction and arterial stiffening. However, there is a lack of knowledge about the role of MR-induced adipose tissue inflammation in the genesis of vascular dysfunction in women. In this study, we hypothesize that MR activation in myeloid cells contributes to angiotensin II (Ang II)-induced aortic stiffening and endothelial dysfunction in females via increased pro-inflammatory (M1) macrophage polarization. Female mice lacking MR in myeloid cells (MyMRKO) were infused with Ang II (500 ng/kg/min) for 4 weeks. This was followed by determinations of aortic stiffness and vasomotor responses, as well as measurements of markers of inflammation and macrophage infiltration/polarization in different adipose tissue compartments. MyMRKO mice were protected against Ang II-induced aortic endothelial stiffening, as assessed via atomic force microscopy in aortic explants, and vasorelaxation dysfunction, as measured by aortic wire myography. In alignment, MyMRKO mice were protected against Ang II-induced macrophage infiltration and M1 polarization in visceral adipose tissue (VAT) and thoracic perivascular adipose tissue (tPVAT). Collectively, this study demonstrates a critical role of MR activation in myeloid cells in the pathogenesis of vascular dysfunction in females associated with pro-inflammatory macrophage polarization in VAT and tPVAT. Our data have potential clinical implications for the prevention and management of cardiovascular disease in women, who are disproportionally at higher risk for poor outcomes.

Tolerance of intimate partner violence and sexist attitudes among health sciences students from three Spanish universities / Tolerancia de la violencia en la pareja y las actitudes sexistas entre estudiantes universitarios/as de ciencias de la salud de tres universidades españolas

OBJECTIVE: To explore university health science students' intimate partner violence tolerance and sexist attitudes and to examine their trend throughout the academic years. METHODS: Cross-sectional study of students of both sexes of the degrees of medicine, nursing, and psychology from three selected Spanish universities (n = 1,322). Data were collected anonymously using two validated scales: the Dating Violence Questionnaire (DVQ-R) and the Gender Role Attitudes Scale (GRAS). Logistic regressions were used to compare tolerance toward violence and sexist attitudes across the degrees. The evolution of these variables throughout different academic courses was assessed. RESULTS: Of the sample, 62.8% were tolerant towards intimate partner violence. The percentage of tolerant students was significantly higher in Psychology (75.9%) than in Nursing (57.7%) and Medicine Degrees (60.3%). A higher percentage of sexist students was also found in psychology students (80.8%) than in nursing (62.2%) and medicine students (62.7%).compared to the first-year students, female medicine students of the last courses were less tolerant to intimate partner violence (p-trend <0.001), and male medicine students had less sexist attitudes (p-trend = 0.002). CONCLUSIONS: Tolerance of intimate partner violence and sexist attitudes were very high, especially among psychology students. These indicators were significantly better among medicine students of higher courses, suggesting a positive effect of medical training. Intimate partner violence in the university education of the future health professionals should be addressed

OBJETIVO: Explorar la tolerancia de la violencia en la pareja y las actitudes sexistas en estudiantes universitarios/as de ciencias de la salud, y estudiar su tendencia a lo largo de los cursos. MÉTODO: Estudio transversal en estudiantes de ambos sexos de medicina, enfermería y psicología de tres universidades españolas (n = 1322). Se utilizaron dos escalas validadas y anónimas: Cuestionario de Violencia entre Novios-Revisado (DVQ-R) y Escala de Actitudes de Rol de Género (EARG). Mediante regresiones logísticas se compararon la tolerancia al abuso y las actitudes sexistas entre titulaciones, y se estudió su evolución a lo largo de la formación académica. RESULTADOS: El 62,8% se mostraron tolerantes ante la violencia en la pareja. El porcentaje de estudiantes tolerantes fue significativamente más elevado en psicología (75,9%) que en enfermería (57,7%) y medicina (60,3%). También se encontró un mayor porcentaje de estudiantes sexistas en psicología (80,8%) que en enfermería (62,2%) y medicina (62,7%). En comparación con estudiantes de nuevo acceso, las estudiantes de medicina de los últimos cursos fueron menos tolerantes a la violencia (p <0,001) y los estudiantes de medicina varones tuvieron menos actitudes sexistas (p = 0,002). CONCLUSIONES: La tolerancia de la violencia en la pareja y las actitudes sexistas fueron muy elevadas, especialmente en psicología. Estos indicadores fueron significativamente mejores en estudiantes de medicina de los cursos superiores, lo que sugiere un efecto positivo de la formación médica. Se debería mejorar el abordaje de la violencia en la pareja en la formación universitaria de los/las futuros/as profesionales sanitarios/as

Tolerance of intimate partner violence and sexist attitudes among health sciences students from three Spanish universities.

OBJECTIVE: To explore university health science students' intimate partner violence tolerance and sexist attitudes and to examine their trend throughout the academic years. METHODS: Cross-sectional study of students of both sexes of the degrees of medicine, nursing, and psychology from three selected Spanish universities (n = 1,322). Data were collected anonymously using two validated scales: the Dating Violence Questionnaire (DVQ-R) and the Gender Role Attitudes Scale (GRAS). Logistic regressions were used to compare tolerance toward violence and sexist attitudes across the degrees. The evolution of these variables throughout different academic courses was assessed. RESULTS: Of the sample, 62.8% were tolerant towards intimate partner violence. The percentage of tolerant students was significantly higher in Psychology (75.9%) than in Nursing (57.7%) and Medicine Degrees (60.3%). A higher percentage of sexist students was also found in psychology students (80.8%) than in nursing (62.2%) and medicine students (62.7%). Compared to the first-year students, female medicine students of the last courses were less tolerant to intimate partner violence (p-trend <0.001), and male medicine students had less sexist attitudes (p-trend = 0.002). CONCLUSIONS: Tolerance of intimate partner violence and sexist attitudes were very high, especially among psychology students. These indicators were significantly better among medicine students of higher courses, suggesting a positive effect of medical training. Intimate partner violence in the university education of the future health professionals should be addressed. .

Colombia y Uruguay ante la COVID-19: datos epidemiológicos, medidas generales y en la práctica odontológica / Colombia and Uruguay in the face of COVID-19: epidemiological data, general and dental practice measures

Objetivo: analizar los datos epidemiológicos y las medidas generales en la práctica odontológica tomadas por Colombia y Uruguay durante la pandemia por la COVID-19. Métodos: este trabajo es una revisión de la literatura. Se consultaron las bases de datos "PubMed", "Scielo" y el motor de búsqueda "Google Scholar". También se consultaron bases de datos de libre acceso como google, our world in data y las páginas oficiales de la Organización Mundial de la Salud, el Ministerio de Salud de Colombia, el Instituto Nacional de Salud y el Ministerio de Salud Pública de Uruguay. Se realizó un análisis descriptivo del comportamiento epidemiológico y de las medidas tomadas en la práctica odontológica en los dos países. Resultados: en Colombia, a diario, se presentan más de 3.000 casos nuevos y más de 100 muertes. Lo contrario ocurre en Uruguay, que ha logrado disminuir las tasas de contagio y de muertes, presentando menos de 10 casos y ninguna muerte diaria. Los profesionales de la salud han incrementado su lucha; los odontólogos se encuentran expuestos por su proximidad al momento de la atención, en el contacto con saliva, sangre y la generación de aerosoles, por lo que han modificado los protocolos de bioseguridad buscando prevenir los contagios asociados a la prestación de servicio. Conclusiones: Uruguay ha tenido un comportamiento ejemplar ante la pandemia; en Colombia los resultados son preocupantes dado el crecimiento exponencial. En ambos países la práctica odontológica se ha visto afectada y se han implementado nuevos protocolos para proteger a profesionales y pacientes.

Objective: Analyze the epidemiological data, general and dental practice measures taken by Colombia and Uruguay in the face of the COVID-19 pandemic. Methods: This study is a review. We search in the databases "PubMed", "Scielo" and in the search engine "Google Scholar". Also, were consulted the free databases like google, our world in data and the official pages of the World Health Organization, Ministry of Health of Colombia, The National institute of health and the Ministry of public Health of Uruguay. A descriptive analysis of the epidemiological behavior and the measures taken of dental practice in both countries was done. Results: Colombia had more than 3,000 new cases and more than 100 deaths daily. The opposite occurs in Uruguay, which has managed to decrease the infection and death rates, presenting less than 10 cases and no daily deaths. The struggles experienced by health professionals has increased, dentists are exposed, by their proximity at the time of care, to contact with saliva, blood and the generation of aerosols, therefore, they have modified the biosafety protocols, seeking to prevent infections associated with the practice. Conclusions: Uruguay has an exemplary behavior; in Colombia these results are worrying with an exponential growth. In both countries, dental practice has been affected and new protocols have been implemented that seek to protect the professionals and patients.

Sexual Dimorphism in Obesity-Associated Endothelial ENaC Activity and Stiffening in Mice.

Obesity and insulin resistance stiffen the vasculature, with females appearing to be more adversely affected. As augmented arterial stiffness is an independent predictor of cardiovascular disease (CVD), the increased predisposition of women with obesity and insulin resistance to arterial stiffening may explain their heightened risk for CVD. However, the cellular mechanisms by which females are more vulnerable to arterial stiffening associated with obesity and insulin resistance remain largely unknown. In this study, we provide evidence that female mice are more susceptible to Western diet-induced endothelial cell stiffening compared with age-matched males. Mechanistically, we show that the increased stiffening of the vascular intima in Western diet-fed female mice is accompanied by enhanced epithelial sodium channel (ENaC) activity in endothelial cells (EnNaC). Our data further indicate that: (i) estrogen signaling through estrogen receptor α (ERα) increases EnNaC activity to a larger extent in females compared with males, (ii) estrogen-induced activation of EnNaC is mediated by the serum/glucocorticoid inducible kinase 1 (SGK-1), and (iii) estrogen signaling stiffens endothelial cells when nitric oxide is lacking and this stiffening effect can be reduced with amiloride, an ENaC inhibitor. In aggregate, we demonstrate a sexual dimorphism in obesity-associated endothelial stiffening, whereby females are more vulnerable than males. In females, endothelial stiffening with obesity may be attributed to estrogen signaling through the ERα-SGK-1-EnNaC axis, thus establishing a putative therapeutic target for female obesity-related vascular stiffening.

Evaluation of intervention measures at different stages of the production chain in Argentinian exporting abattoirs.

Antimicrobial treatments could help to decrease the transmission of microorganisms to beef carcasses and abattoir environments. The aim of this study was to evaluate the effectiveness of interventions in reducing Shiga toxin genes (stx1 and stx2) presence in a commercial abattoir. Intervention measures included the application of electrolytically generated hypochlorous acid to steer pens (experiment 1), chlorinated water, electrolytically generated hypochlorous acid, and isoclor to steer pens (experiment 2), electrolytically generated hypochlorous acid to knocking pens (experiment 3), and aqueous ozone and electrolytically generated hypochlorous acid onto beef carcasses (experiment 4). Detection of stx in samples was performed with BAX® System Real-Time PCR Assay. Our results showed that treatment with pressurized electrolytically generated hypochlorous acid and isoclor were effective to reduce stx presence from hides on steer pens. Although there is no single strategy to ensure the reduction of stx presence in a commercial abattoir, the combined application of several antimicrobial interventions would be ideal.

Comparison and cost analysis of three protocols for mobilization and apheresis of haematopoietic progenitor cells.

INTRODUCTION: Autologous bone marrow transplantation is a component of the malignant hemopathy therapy. The preferred mobilization and collection method is apheresis. The aim of this study is to compare three protocols analyzing the effect of plerixafor, higher dose of G-CSF and large volume leukapheresis (LVL). MATERIALS AND METHODS: A retrospective cohort study including 119 patients referred for mobilization. Three protocols were compared: (a) G-CSF 10 µg/kg/day subcutaneous (sc) × 4 days mobilizing 1 to 1.5 blood volumes. (b) G-CSF 10 µg/kg/day sc × 4 days + plerixafor 0.24 mg/kg/day sc preventively or as a rescue agent mobilizing 1 to 1.5 blood volumes. (c) G-CSF 20 µg/kg/day sc × 4 days ± plerixafor 0.24 mg/kg/day sc preventively or as a rescue agent mobilizing 3 to 4 blood volumes. RESULTS: The average number of days of apheresis was reduced to 1.37 with protocol 3. The average cost per patient was reduced by 67% compared with protocol 2 and increased by only 5% compared with protocol 1, reducing the failure rate to 0%. CONCLUSION: Adding preemptive or rescue plerixafor (protocol 2) to G-CSF 10 µg/kg/day alone (protocol 1) did not improve the days of apheresis nor the number of CD34+ cells collected but had higher cost and failure rate. Using LVL, plerixafor and G-CSF 20 µg/kg/day (protocol 3) decreased the number of sessions to 1.37, reduced the failure rate to 0% and led to a significant increase in the number of CD34+ cells collected without toxicity and with a similar cost to protocol 1.

How work setting and job experience affect professional nurses' values.

BACKGROUND:: The development of professional values in nursing is directly related to quality and ethical clinical practise and may also increase practitioner and patients' satisfaction. Some factors, such as work setting or work experience, can influence the importance granted to the professional values of nursing. OBJECTIVES:: To compare in primary care nurses and hospital care nurses the importance granted to professional values and to contrast this perception as a function of professional experience. RESEARCH DESIGN, PARTICIPANTS AND RESEARCH CONTEXT:: Descriptive cross-sectional study. Participants were 380 nursing professionals from the public health system (primary care and hospital care). Three dimensions were analysed: ethics, professional expertise and professional mastery. Data were collected from January to June 2015. ETHICAL CONSIDERATIONS:: We obtained permission from the Ethics Committee and participants' informed consent. FINDINGS:: Hospital care professionals attached more importance to all the values analysed, regardless of their work experience. Ethical values, such as confidentiality and respect for the person, were considered to be very important in both systems. Values related to professional expertise obtained lower scores, especially in primary care. In general, professionals with more than 20 years' experience granted less importance to the values. CONCLUSION:: The professional setting influenced the importance assigned to professional nursing values, and clear differences were observed between primary and hospital care. The domain of ethics was considered the most important. It is necessary to reflect on the significance attributed to professional values, especially in more expert nursing staff.

Actitudes sexistas y reconocimiento del maltrato en parejas jóvenes / Sexist attitudes and recognition of abuse in young couples

OBJETIVO: Explorar la asociación entre las actitudes de rol de género y el reconocimiento del maltrato en jóvenes. DISEÑO: Estudio transversal. Emplazamiento: Cincuenta y siete centros de educación secundaria, formación profesional y universitaria de 5 provincias españolas (Huelva, Sevilla, A Coruña, Pontevedra y Asturias). PARTICIPANTES: Un total de 4.337 estudiantes de entre 15 y 26 años (40,6% chicos y 59,4% chicas) que mantuvieron una relación de pareja durante un mes o más. Mediciones principales: Se utilizó la Escala de Actitudes de Rol de Género, con 20 indicadores de actitudes igualitarias o sexistas a nivel familiar, social y laboral. También se estudió si la persona estaba en una situación de maltrato percibido (MP), maltrato no percibido (MNP) o no maltrato (NM). RESULTADOS: En el conjunto de la muestra, el 68,6% se declaró NM, el 26,4% vivía una situación de MNP y el 5,0% reconoció sufrir maltrato. El MP fue más frecuente entre las chicas (6,3%), en los/as ≥ 18 años (6,4%) y en el alumnado universitario (6,9%). El MNP fue más habitual en los chicos (30,2%). Las actitudes más sexistas se encontraron en la dimensión laboral, y especialmente en varones y en adolescentes (15-17 años). Las actitudes menos sexistas se asociaron con menor probabilidad de vivir situaciones de MNP (odds ratio = 0,71; p-tendencia < 0,001). CONCLUSIONES: El sexismo parece dificultar el reconocimiento del maltrato. Lograr la equidad de género en la adolescencia y juventud es imprescindible. Los esfuerzos deberían centrarse en los varones, por ser el grupo con actitudes más sexistas y con mayor prevalencia de MNP

AIM: To explore the association between gender-role attitudes and the recognition of abuse among adolescents and young adults during dating relationships. DESIGN: Cross-correlation study. LOCATION: 57 schools of secondary education, vocational education and university in five provinces of Spain (Huelva, Seville, A Coruña, Pontevedra and Asturias). PARTICIPANTS: 4,337 students aged between 15 and 26 years (40.6% males and 59.4% female) who had a dating relationship that lasted more than a month. MAIN MEASUREMENTS: The Gender Role Attitudes Scale was used, which consists of 20 indicators of egalitarian or sexism attitudes at the family, social and occupational level. The students also reported whether they suffered from recognized abuse (RA), unperceived abuse (UPA), or not abused (NA). RESULTS: In the whole sample, 68.6% declared themselves NA, 26.4% were under a situation of UPA, and 5.0% were RA. The RA group was more frequent among the females (6.3%), ≥ 18 years (6.4%) and university students (6.9%). UPA was more common in males (30.2%). The most sexist attitudes were found in the occupational dimension and especially in men and adolescents (15-17 years). Less sexist attitudes were associated with a lower probability of experiencing UPA (odds ratio = .71; P-trend < .001). CONCLUSIONS: Sexism seems to hinder the recognition of abuse. Achieving gender equity in adolescence and youth is imperative. Public health efforts should focus on men, as they constitute the group with more sexist attitudes and with higher prevalence of UPA

[Sexist attitudes and recognition of abuse in young couples]. / Actitudes sexistas y reconocimiento del maltrato en parejas jóvenes.

AIM: To explore the association between gender-role attitudes and the recognition of abuse among adolescents and young adults during dating relationships. DESIGN: Cross-correlation study. LOCATION: 57 schools of secondary education, vocational education and university in five provinces of Spain (Huelva, Seville, A Coruña, Pontevedra and Asturias). PARTICIPANTS: 4,337 students aged between 15 and 26 years (40.6% males and 59.4% female) who had a dating relationship that lasted more than a month. MAIN MEASUREMENTS: The Gender Role Attitudes Scale was used, which consists of 20 indicators of egalitarian or sexism attitudes at the family, social and occupational level. The students also reported whether they suffered from recognized abuse (RA), unperceived abuse (UPA), or not abused (NA). RESULTS: In the whole sample, 68.6% declared themselves NA, 26.4% were under a situation of UPA, and 5.0% were RA. The RA group was more frequent among the females (6.3%), ≥18 years (6.4%) and university students (6.9%). UPA was more common in males (30.2%). The most sexist attitudes were found in the occupational dimension and especially in men and adolescents (15-17 years). Less sexist attitudes were associated with a lower probability of experiencing UPA (odds ratio=.71; P-trend<.001). CONCLUSIONS: Sexism seems to hinder the recognition of abuse. Achieving gender equity in adolescence and youth is imperative. Public health efforts should focus on men, as they constitute the group with more sexist attitudes and with higher prevalence of UPA.

Seasonal Changes in the Endosymbiotic Consortia of Aphids from the Genus Cinara.

Buchnera aphidicola is the primary endosymbiont of aphids with which it maintains an obligate mutualistic symbiotic relationship. Insects also maintain facultative symbiotic relationships with secondary symbionts, and Serratia symbiotica is the most common in aphids. The presence of both symbionts in aphids of the subfamily Lachninae has been widely studied by our group. We examined two closely related aphids, Cinara tujafilina and C. cedri in the present study. Even though both B. aphidicola strains have similar genome sizes and gene contents, the genomes of the two S. symbiotica strains were markedly different. The SCc strain has the smallest genome known for this species, while SCt possesses a larger genome in an intermediate stage between the facultative S. symbiotica of Acyrthosiphon pisum (SAp) and the co-obligate S. symbiotica SCc.Aphids are vulnerable to high temperatures. Previous studies indicated that S. symbiotica SAp confers resistance to heat-shock stress. In order to clarify whether S. symbiotica strains from genus Cinara also play a role in heat stress protection, we performed a quantitative determination of the consortium Buchnera/Serratia from two geographically close populations, each of which belonged to the Cinara species examined, over two years in natural environments. We found no variation in the consortium from our C. cedri population, but a positive correlation between both endosymbiont densities and average daily temperatures in the C. tujafilina population. Even though S. symbiotica SCt may retain some protective role against heat stress, this does not appear to be due to the release of protective metabolites by cell lysis.

Consumo de tabaco y alcohol según la jornada laboral en España / Tobacco and alcohol consumption according to workday in Spain

Objetivo: Explorar la asociación entre el consumo de tabaco y alcohol y el tipo de jornada laboral en población española. Métodos: Estudio transversal sobre una muestra de residentes en España, adultos y laboralmente activos (N=8155). La fuente de datos fue la Encuesta Nacional de Salud 2011-2012. Se recogió información sobre el tipo de jornada laboral (mañana, tarde, noche, partida, reducida y a turnos) y el consumo regular de tabaco y alcohol. También se incluyeron variables sociodemográficas y otras relacionadas con la salud y la actividad laboral. Se calcularon lasodds ratios ajustadas (ORa) y sus intervalos de confianza del 95% (IC95%) mediante regresiones log-binomial. Resultados: El 32,1% de las personas encuestadas fumaba regularmente, en especial aquellas que trabajaban en turno nocturno (43,5%). El 54,8% de la población trabajadora tenía un consumo alcohólico moderado y el 1,5% de riesgo. El tipo de jornada con más bebedores moderados y de riesgo fue la partida, con un 57,6% y un 1,8% respectivamente. La ORa de ser fumador fue mayor en los que trabajaban por la noche (OR=1,56; IC95%: 1,01-2,46). Ningún turno de trabajo se asoció significativamente con el consumo de alcohol. Conclusiones: El turno de trabajo nocturno se asoció con el consumo regular de tabaco. Los servicios de salud laboral deberían extremar la vigilancia de este grupo de población trabajadora, incluyéndolo sistemáticamente en programas para controlar el tabaquismo y las enfermedades relacionadas. En el futuro, estudiar las razones para este fenómeno podría aportar claves para lograr el éxito preventivo y terapéutico (AU)

Objective: To examine the association between smoking and alcohol consumption and the type of working day in the Spanish population. Methods: Cross-sectional study among employees residing in Spain aged >18 years (N=8,736). We took data from the National Health Survey (2011-2012). Information was collected on the type of working day (morning, afternoon, evening, part-time, reduced hours, and shift-work) and smoking and drinking habits. Demographic characteristics and health- and work-related factors were also taken into account. Adjusted odds ratios (aOR) and 95% confidence intervals (95%CI) were calculated through log-binomial regressions. Results: Among respondents, 32.1% smoked regularly, especially those working the night shift (43.5%). Moderate alcohol consumption was found in 54.8% of workers and excessive consumption in 1.5%. Most of the moderate and heavy drinkers worked part-time, with 57.6% and 1.8% respectively. The aOR of being a smoker was higher among night workers (OR=1.58; 95% CI: 1.01-2.46). None of the work shifts were significantly associated with alcohol consumption. Conclusions: Night shift work was associated with regular smoking. This collective of workers should be monitored closely by occupational health services and regularly undergo programs to control tobacco consumption and smoking-related diseases. Additional research to elucidate the reasons for this association could help to achieve preventive and therapeutic success (AU)

[Tobacco and alcohol consumption according to workday in Spain]. / Consumo de tabaco y alcohol según la jornada laboral en España.

OBJECTIVE: To examine the association between smoking and alcohol consumption and the type of working day in the Spanish population METHODS: Cross-sectional study among employees residing in Spain aged >18 years (N=8,736). We took data from the National Health Survey (2011-2012). Information was collected on the type of working day (morning, afternoon, evening, part-time, reduced hours, and shift-work) and smoking and drinking habits. Demographic characteristics and health- and work-related factors were also taken into account. Adjusted odds ratios (aOR) and 95% confidence intervals (95%CI) were calculated through log-binomial regressions. RESULTS: Among respondents, 32.1% smoked regularly, especially those working the night shift (43.5%). Moderate alcohol consumption was found in 54.8% of workers and excessive consumption in 1.5%. Most of the moderate and heavy drinkers worked part-time, with 57.6% and 1.8% respectively. The aOR of being a smoker was higher among night workers (OR=1.58; 95% CI: 1.01-2.46). None of the work shifts were significantly associated with alcohol consumption. CONCLUSIONS: Night shift work was associated with regular smoking. This collective of workers should be monitored closely by occupational health services and regularly undergo programs to control tobacco consumption and smoking-related diseases. Additional research to elucidate the reasons for this association could help to achieve preventive and therapeutic success.

Violencia de género en estudiantes de enfermería durante sus relaciones de noviazgo / Gender violence in nursing students during their dating relationships

Objetivo: Determinar la prevalencia de violencia de género (VG) en futuras enfermeras e identificar su relación con variables sociodemográficas y personales (apoyo social y autoestima) y la percepción que tienen sobre su función como profesionales de atención primaria. Diseño: Estudio descriptivo transversal. Emplazamiento: Facultad de Medicina y Ciencias de la Salud (Universidad de Oviedo). Participantes: Estudiantes del Grado en Enfermería. Mediciones principales: Se utilizó el cuestionario CUVINO, que mide 42 indicadores conductuales y 8 factores de VG y permite identificar conductas de violencia técnica y declarada durante el noviazgo y otras situaciones relacionadas. Se midió también el nivel de autoestima social, el número de apoyos y variables relacionadas con la función enfermera. Resultados: Con criterio de «tolerancia cero», el 85,8% sufrió alguna situación de violencia técnica, mayoritariamente por «desapego» (73,3%) y «coerción» (66,3%), aunque casi 1 de cada 5 sufrió también violencia «física» (18,3%). El 9,0% declaró haber sido realmente maltratada, percepción relacionada con la proporción global de estudiantes que se sintieron atrapadas (31,7%, p < 0,001) y con miedo (13,8%, p < 0,001) y con una mayor demora en la ruptura de la relación (13,2 meses vs. 3,6; p < 0,05). Sufrir violencia técnica se asoció con menor número de apoyos (p < 0,05) y con menor autoestima social (p < 0,01). La formación en maltrato mejora su reconocimiento. Conclusiones: La prevalencia de situaciones de maltrato entre las futuras enfermeras es muy elevada y su reconocimiento deficiente. La formación académica podría mejorar la percepción del problema, por lo que sería deseable reforzar su inclusión en el currículo y el entrenamiento de habilidades concretas (AU)

Aim: To determine the prevalence of gender violence (GV) suffered by student nurses and to identify its relationship with some sociodemographic and personal variables (social support and self-esteem) and their perception about their role as primary care providers. Design: Cross-sectional study. Location: Faculty of Medicine and Health Sciences (University of Oviedo). Participants: Nursing students. Main measurements: The CUVINO questionnaire was used. It measures 42 behavioral indicators and 8 GV factors and allows to identify technical and perceived violence during courtship and other related situations. The level of social self-esteem, support and variables related with the nursing role were also measured. Results: Using the standard of “zero tolerance”, 85.8% suffered technical violence, mainly by “detachment” and “coercion” (73.3%), while almost 1 in 5 also suffered “physical” violence (18.3%). A lower percentage (9.0%) reported having been really ill-treated, perception related to the global proportion of students who felt trapped (31.7%, P <0.001), felt fear (13.8%, P <0.001) and a further delay in breaking the relationship (13.2 months vs. 3.6; P <0.05). Suffering technical violence was associated with less social support (P <0.05) and less social self-esteem (P <0.01). Academic training in GV improves recognition. Conclusions: The prevalence of GV situations between student nurses is very high and recognition is poor. Academic training could improve the perception of the problem, thus it would be desirable to strengthen its inclusion in the university curriculum, together with specific skills training (AU)

Gout, hyperuricaemia, sleep apnoea-hypopnoea syndrome and vascular risk.

OBJECTIVE: This study aimed to evaluate cardiovascular (CV) and atherothrombosis risk factors in patients with gout and hyperuricaemia with suspected sleep apnoea-hypopnoea syndrome (SAHS) compared with a control group of subjects with knee OA and SAHS. METHODS: Clinical information on CV risk factors and atherothrombosis was collected in a rheumatology department in patients with gout and hyperuricaemia and suspicion of SAHS. Confirmation polysomnography that registered apnoea-hypopnoea index (AHI) and oxygen saturation during sleep (SaO2) was performed. The control group consisted of patients with OA and polysomnographically confirmed SAHS. RESULTS: In the gout patient group (54 patients, 48 men), CV risk factors were found in 77.8% and evidence of atherothrombosis in 46.3%. In the OA group (36 patients, 27 men), CV risk factors were found in 66.7% and evidence of atherothrombosis in 0%. SAHS diagnosis was confirmed by polysomnography in 88.9% of patients. AHI showed mild, moderate and severe SAHS in 12%, 26% and 66% of the gout patients and 45%, 24% and 30% of the OA patients, respectively. SaO2 was 90.18% in the gout group and 91.26% in the OA group. CONCLUSION: Patients with gout and hyperuricaemia and suspicion of SAHS had polysomnographically confirmed SAHS in 88.9% of cases. These patients had more severe forms of SAHS and a greater prevalence of documented atherothrombotic disease compared with a control group with OA.

[Gender violence in nursing students during their dating relationships]. / Violencia de género en estudiantes de enfermería durante sus relaciones de noviazgo.

AIM: To determine the prevalence of gender violence (GV) suffered by student nurses and to identify its relationship with some sociodemographic and personal variables (social support and self-esteem) and their perception about their role as primary care providers. DESIGN: Cross-sectional study. LOCATION: Faculty of Medicine and Health Sciences (University of Oviedo). PARTICIPANTS: Nursing students. MAIN MEASUREMENTS: The CUVINO questionnaire was used. It measures 42 behavioral indicators and 8 GV factors and allows to identify technical and perceived violence during courtship and other related situations. The level of social self-esteem, support and variables related with the nursing role were also measured. RESULTS: Using the standard of "zero tolerance", 85.8% suffered technical violence, mainly by "detachment" and "coercion" (73.3%), while almost 1 in 5 also suffered "physical" violence (18.3%). A lower percentage (9.0%) reported having been really ill-treated, perception related to the global proportion of students who felt trapped (31.7%, P <.001), felt fear (13.8%, P <.001) and a further delay in breaking the relationship (13.2 months vs. 3.6; P <.05). Suffering technical violence was associated with less social support (P <.05) and less social self-esteem (P <.01). Academic training in GV improves recognition. CONCLUSIONS: The prevalence of GV situations between student nurses is very high and recognition is poor. Academic training could improve the perception of the problem, thus it would be desirable to strengthen its inclusion in the university curriculum, together with specific skills training.