Effects of Crossfit® and street running practice on anthropometric, lipids parameters, cardiorespiratory fitness and sleep quality.

BACKGROUND: Street running (SR) and CrossFit® (CF) have different characteristics ranging from aerobic training to high intensity. This study aimed to describe the subject's physical training, anthropometric and lipid parameters, cardiorespiratory fitness and sleep quality and duration. METHODS: Cross-sectional, study, that collected personal data, Pittsburgh Sleep Quality Index (PSQI), anthropometric assessment, cardiorespiratory fitness, and lipid profile. The subjects were separated in CF group (CFG) and SR group (SRG). RESULTS: The SRG training frequency was lower (P=0.006), had better maximum oxygen consumption (V̇O2max) levels (P<0.001). 59.3% of the SRG had excellent V̇O2max. Cardiorespiratory fitness (49.97 mL/kg/min; P=0.001) and Body Mass Index (BMI) were positively related in SR (P=0.031). An inverse correlation was found between V̇O2max and body fat percentage (BF%) (SRG: P=0.001; CFG: P=0.013). Sleep duration is strongly and inversely associated with PSQI. There was a correlation between total cholesterol (TC) and high-density lipoprotein cholesterol (P=0.020), TC and triglycerides (TGs) (P=0.029) and levels of TGs and BMI (P=0.008) in SRG. In the CFG group, there was a correlation of TC between TGs levels (P=0.025), light-density lipoprotein cholesterol (P<0.001) and BMI (P=0.050). CONCLUSIONS: The SR have a higher V̇O2max although they train less than the CF practitioners regardless of factors such as BF%, gender and age.

Moderating Role of Physical Fitness in the Association Between TV Time and Adiposity Parameters in Adolescents.

PURPOSE: To identify whether physical fitness (PF) components play a moderating role in the relationship between TV time and adiposity levels. DESIGN: Cross-sectional study. SETTING: Few studies have examined if different PF levels modify the association between TV time and adiposity in adolescents. Studies often focus on the isolated relationships between obesity and TV time, or obesity and PF levels. SUBJECTS: 1071 adolescents (617 girls), aged 12 to 17 years. MEASURES: Cardiorespiratory fitness (CRF), abdominal muscular endurance, and lower limb strength were evaluated using the protocols of the Projeto Esporte Brasil fitness testing battery. TV time was obtained using a self-reported questionnaire. Body mass index (BMI) and waist circumference (WC) were also assessed. Moderation analyses were conducted through multiple linear regression models with the following associations tested in different models: PF components, TV time, and interaction (PF component x TV time) with adiposity parameters (BMI and WC). RESULTS: A significant interaction term was found for CRF and TV time in the association with both WC (ß: -.005; 95% CI: -.009; -.001; P = .012) and BMI (ß: -.002; 95% CI: -.004; -.001; P = .009). CONCLUSION: CRF moderates the relationship between TV time and adiposity measures in this cross-sectional analysis. These data support strategies looking at increasing physical activity levels to improve CRF and avoid the development of excess abdominal obesity and excess weight.

First inter-laboratory study of a Supercritical Fluid Chromatography method for the determination of pharmaceutical impurities.

Supercritical Fluid Chromatography (SFC) has known a strong regain of interest for the last 10 years, especially in the field of pharmaceutical analysis. Besides the development and validation of the SFC method in one individual laboratory, it is also important to demonstrate its applicability and transferability to various laboratories around the world. Therefore, an inter-laboratory study was conducted and published for the first time in SFC, to assess method reproducibility, and evaluate whether this chromatographic technique could become a reference method for quality control (QC) laboratories. This study involved 19 participating laboratories from 4 continents and 9 different countries. It included 5 academic groups, 3 demonstration laboratories at analytical instrument companies, 10 pharmaceutical companies and 1 food company. In the initial analysis of the study results, consistencies within- and between-laboratories were deeply examined. In the subsequent analysis, the method reproducibility was estimated taking into account variances in replicates, between-days and between-laboratories. The results obtained were compared with the literature values for liquid chromatography (LC) in the context of impurities determination. Repeatability and reproducibility variances were found to be similar or better than those described for LC methods, and highlighted the adequacy of the SFC method for QC analyses. The results demonstrated the excellent and robust quantitative performance of SFC. Consequently, this complementary technique is recognized on equal merit to other chromatographic techniques.