Examining the effects of paper type, pen type, writing pressure and angle of intersection on white and dark dominance in ESDA impressions of sequenced strokes--an application of the likelihood ratio.

Questioned document casework can occasionally involve the sequencing of impressions. Although some conclusions can be drawn from looking at ESDA traces of intersections there is currently no way of assessing the strength of the observations made in any given case. Using a range of paper and pen types this work examines points of intersection and evaluates the results statistically in order judge the value of the results obtained.

The effect of nitrostyrene on cell proliferation and macrophage immune responses.

The use of mood enhancing drugs such as amphetamine and ecstasy are now prevalent in society. These compounds are known to produce serious psychological and physiological problems in users, which can, in some circumstances result in death. While there has been much research into the effects of these drugs on the body, little if any research has investigated the effect of the side products and synthetic reaction by-products which are a consequence of there illegal production. In the study the effects of nitrostyrene, a reaction by-product in one of the routes to synthesis of amphetamine sulphate, on cell viability and macrophage function was determined. Treatment with nitrostyrene at doses >0.75 microg/mL had a significant suppressive effect on the proliferation of stomach cancer lines. Treatment of macrophages with doses as high as 10 microg/mL did not effect cell viability. Nitrostyrene treatment of macrophages, stimulated with IFN gamma and LPS, resulted in a dose dependent differential inhibition in IL12, IL6 and nitrite production, even using doses < 0.5 microg/mL. Thus ranking of the three, on the basis of the suppressive effect obtained, is IL12 > nitrite > IL6. Thus ingestion of nitrostyrene contaminated ecstasy is likely to have a adverse effect on the immune responses of the recreational user.