Identification of Toxicants from a Highly C10 -C40 -Contaminated Sediment Influenced by the Wood Industry: Petroleum Hydrocarbons or Biogenic Organic Compounds?

Sediment from a log pond located in south Finland contained 15 000 to 50 000 mg/kg dry weight of C10 -C40 hydrocarbons. It was unclear whether they originated from the hydraulic fluid of the log hoist or the wood extractives. In the present study, methods of effect-directed analysis were used for the identification of toxicants. A combination of fractionation, biotesting, and chemical analyses revealed that the key toxicant of log pond sediment was retene, a dialkyl-substituted phenanthrene derived from wood resin acids. In addition, the most toxic fraction included 3 other wood-originated diterpenic compounds. Typical wood extractives such as sesquiterpenes and odd-carbon number alkanes in the range C21 -C33 were identified in the fraction, which showed minor genotoxic potency. The most polar fraction contained triterpenes and showed estrogenic activity. No evidence for the presence of hydraulic fluid in sediment was found. The study also indicated that in cases where the organic matter content of sediment or soil is high, using the results of standard mineral oil analysis in risk management can lead to incorrect actions because standard methods do not differentiate petroleum hydrocarbons from biogenic hydrocarbons. Environ Toxicol Chem 2019;9999:1-11. © 2019 SETAC.

Biostimulation proved to be the most efficient method in the comparison of in situ soil remediation treatments after a simulated oil spill accident.

The use of in situ techniques in soil remediation is still rare in Finland and most other European countries due to the uncertainty of the effectiveness of the techniques especially in cold regions and also due to their potential side effects on the environment. In this study, we compared the biostimulation, chemical oxidation, and natural attenuation treatments in natural conditions and pilot scale during a 16-month experiment. A real fuel spill accident was used as a model for experiment setup and soil contamination. We found that biostimulation significantly decreased the contaminant leachate into the water, including also the non-aqueous phase liquid (NAPL). The total NAPL leachate was 19 % lower in the biostimulation treatment that in the untreated soil and 34 % lower in the biostimulation than oxidation treatment. Soil bacterial growth and community changes were first observed due to the increased carbon content via oil amendment and later due to the enhanced nutrient content via biostimulation. Overall, the most effective treatment for fresh contaminated soil was biostimulation, which enhanced the biodegradation of easily available oil in the mobile phase and consequently reduced contaminant leakage through the soil. The chemical oxidation did not enhance soil cleanup and resulted in the mobilization of contaminants. Our results suggest that biostimulation can decrease or even prevent oil migration in recently contaminated areas and can thus be considered as a potentially safe in situ treatment also in groundwater areas.

NAPL migration and ecotoxicity of conventional and renewable fuels in accidental spill scenarios.

Fuels derived from non-petroleum renewable resources have raised interest due to their potential in replacing petroleum-based fuels, but information on their fate and effects in the terrestrial and aquatic environments in accidental spill scenario is limited. In this study, migration of four fuels (conventional diesel, conventional gasoline, renewable diesel NExBTL, and ethanol-blended gasoline RE85 containing maximum 85% ethanol) as non-aqueous phase liquids (NAPL) in soil was demonstrated in a laboratory-scale experiment. Ecotoxicity data was produced for the same fuels. There was no significant difference in migration of conventional and renewable diesel, but gasoline migrated 1.5 times deeper and 7-9 times faster in sand than diesel. RE85 spread horizontally wider but not as deep (p < 0.05) as conventional gasoline. Conventional gasoline was the most toxic (lethal concentration [LC50] 20 mg/kg total hydrocarbon content [THC]) among the studied fuels in soil toxicity test with earthworm Eisenia fetida followed by ethanol-blended gasoline (LC50 1,643 mg/kg THC) and conventional diesel (LC50 2,432 mg/kg THC), although gasoline evaporated fast from soil. For comparison, the toxicity of the water-accommodated fractions (WAF) of the fuels was tested with water flea Daphnia magna and Vibrio fischeri, also demonstrating groundwater toxicity. The WAF of conventional gasoline and RE85 showed almost similar toxicity to both the aquatic test species. EC50 values of 1:10 (by volume) WAF were 9.9 %WAF (gasoline) and 9.3 %WAF (RE85) to D. magna and 9.3 %WAF (gasoline) and 12.3 %WAF (RE85) to V. fischeri. Low solubility decreased toxicity potential of conventional diesel in aquatic environment, but direct physical effects of oil phase pose a threat to organisms in nature. Renewable diesel NExBTL did not show clear toxicity to any test species.