An epigallocatechin gallate-amorphous calcium phosphate nanocomposite for caries prevention and demineralized enamel restoration.

Biomineralization with amorphous calcium phosphate (ACP) is a highly effective strategy for caries prevention and defect restoration. The identification and interruption of cariogenic biofilm formation during remineralization remains a challenge in current practice. In this study, an epigallocatechin gallate (EGCG)-ACP functional nanocomposite was developed to prevent and restore demineralization by integrating the antibacterial property of EGCG and the remineralization effect of ACP. The synthesized EGCG-ACP showed good biocompatibility with L-929 â€‹cells and human gingival fibroblasts. Under neutral conditions, the sustained release of ACP from EGCG-ACP restored the microstructure and mechanical properties of demineralized enamel. Under acidic conditions, protonated EGCG released from EGCG-ACP exerted a strong antibacterial effect, and the ACP release rate doubled within 4 â€‹h, resulting in the prevention of demineralization in the presence of cariogenic bacteria. The pH-responsive features of EGCG-ACP to promote the protonation of EGCG and ACP release facilitated its performance in remineralization effect to overcome the difficulty of restoring demineralized enamel in a cariogenic acidic environment, which was evidenced by the in vivo experiment carried out in a rat oral cariogenic environment. The results of this study indicate the potential of EGCG-ACP for the prevention of enamel demineralization and provide a theoretical basis its application in populations with high caries risk.

Controlling the size of GO in GO/nAg nanocomposite coatings on orthodontic nickel-titanium alloy toward excellent anti-corrosion, antibacterial, and tribological properties.

High surface friction and lack of antibacterial properties limit the efficiency of fixed metallic orthodontic appliances. Graphene oxide/silver (GO/nAg) nanocomposite coatings were constructed on the surface of a representative nickel-titanium (NiTi) alloy using different sizes of GO via pulse electrodeposition; these coatings were characterized in terms of their microstructure, surface properties, and related biological features. Small-sized GO (SGO) with a lateral size of about 70 nm had more sites for nAg to bind, which helped to form serried and uniformly dispersed nAg; this decreased the coefficient of friction to 0.1, reduced the corrosion current density by ten times, and decreased the amount of corrosive ions, as determined from electrochemical and stress corrosion tests. In the coating containing large-sized GO (LGO), the prolonged growth of nAg resulted in a larger size and scattered distribution, thus having limited optimization in terms of friction and corrosion resistance. Both GO/nAg coatings were biocompatible with L929 cells and adhesion to human gingival fibroblast cells. The LGO/nAg coating released two folds of Ag+ than the SGO/nAg coating initially, while the latter exerted a stable antibacterial effect over seven days against 90% Streptococcus mutans through sustained release. It is suggested that the size of GO could regulate the mechanical and biological properties of GO/nAg coatings. This study aims to improve the efficiency of orthodontic treatment and promote the clinical popularization of multifunctional GO/nAg nanocomposite coatings in the oral environment.

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies.

The rapid degradation of magnesium (Mg) alloy implants erodes mechanical performance and interfacial bioactivity, thereby limiting their clinical utility. Surface modification is among the solutions to improve corrosion resistance and bioefficacy of Mg alloys. Novel composite coatings that incorporate nanostructures create new opportunities for their expanded use. Particle size dominance and impermeability may increase corrosion resistance and thereby prolong implant service time. Nanoparticles with specific biological effects may be released into the peri-implant microenvironment during the degradation of coatings to promote healing. Composite nanocoatings provide nanoscale surfaces to promote cell adhesion and proliferation. Nanoparticles may activate cellular signaling pathways, while those with porous or core-shell structures may carry antibacterial or immunomodulatory drugs. Composite nanocoatings may promote vascular reendothelialization and osteogenesis, attenuate inflammation, and inhibit bacterial growth, thus increasing their applicability in complex clinical microenvironments such as those of atherosclerosis and open fractures. This review combines the physicochemical properties and biological efficiency of Mg-based alloy biomedical implants to summarize the advantages of composite nanocoatings, analyzes their mechanisms of action, and proposes design and construction strategies, with the purpose of providing a reference for promoting the clinical application of Mg alloy implants and to further the design of nanocoatings.

Metal-Based Nanozymes with Multienzyme-Like Activities as Therapeutic Candidates: Applications, Mechanisms, and Optimization Strategy.

Most nanozymes in development for medical applications only exhibit single-enzyme-like activity, and are thus limited by insufficient catalytic activity and dysfunctionality in complex pathological microenvironments. To overcome the impediments of limited substrate availabilities and concentrations, some metal-based nanozymes may mimic two or more activities of natural enzymes to catalyze cascade reactions or to catalyze multiple substrates simultaneously, thereby amplifying catalysis. Metal-based nanozymes with multienzyme-like activities (MNMs) may adapt to dissimilar catalytic conditions to exert different enzyme-like effects. These multienzyme-like activities can synergize to realize "self-provision of the substrate," in which upstream catalysts produce substrates for downstream catalytic reactions to overcome the limitation of insufficient substrates in the microenvironment. Consequently, MNMs exert more potent antitumor, antibacterial, and anti-inflammatory effects in preclinical models. This review summarizes the cellular effects and underlying mechanisms of MNMs. Their potential medical utility and optimization strategy from the perspective of clinical requirements are also discussed, with the aim to provide a theoretical reference for the design, development, and therapeutic application of their catalytic effects.

Graphene oxide induced dynamic changes of autophagy-lysosome pathway and cell apoptosis via TFEB dysregulation in F98 cells.

The extensive application of graphene oxide (GO) nanomaterials increases the risk of their release into the environment, thus posing a threat to the human body. Multiple studies indicate that GO could lead to neurotoxicity, while the intricate biological effects of GO in astrocytes remain unclear. The autophagic disorder was considered an important part of the exposure risk of GO in the application of neuromedicine. This study explored the key regulators mediating the autophagic process in rat astroglioma-derived F98 cells caused by GO, especially the dynamic changes in the cellular physiological state over time. We identified transcription factor EB (TFEB), a critical regulator of the autophagy-lysosome pathway (ALP), as a crucial factor in GO-induced autophagy flux blockade and cell apoptosis. Specifically, the prolonged exposure to GO increased the amount of its cellular internalization, which gradually prevented TFEB from entering the nucleus, thereby leading to the subsequent ALP dysfunction and excessive cell apoptosis. Furthermore, STIP1 homology and U-Box containing protein 1 (STUB1), an E3 ubiquitin ligase, was responsible for GO-triggered TFEB dysregulation, and overexpression of STUB1 helped alleviate GO cytotoxicity. Our study highlights that impaired TFEB activity underlies compromised autophagy flux in GO-induced apoptosis and opens up new avenues for the application of GO-based nanotherapeutics with specific autophagy-regulating properties in the central nervous system.

The design, construction and application of graphene family composite nanocoating on dental metal surface.

Enhancement of the biological and mechanical properties of dental metals is important for accommodation with therapeutic schemes in different stomatological disciplines. Nanocoatings based on graphene family nanomaterials (GFNs) improve the topological structure and physicochemical properties of metal surfaces, endowing them with new properties while maintaining inherent mechanical properties. Nano-composite coatings, composed of GFNs with one or more type of polymer, metal, oxide, and inorganic nonmetallic compound, offer more matching modification schemes to meet multifunctional oral treatment requirements (e.g., anti-bacterial and anti-corrosive activity, osteogenesis and angiogenesis). This review describes recent progress in the development of GFN composite nanocoatings for the modification of dental metals, focus on biological effects in clinical settings. Underlying molecular mechanisms, critical modification schemes, and technical innovation in preparation methods are also discussed. The key parameters of GFN composite nanocoating surface modification are summarized according to effects on cellular responses and antibacterial activity. This review provides a theoretical reference for the optimization of the biological effects and application of GFN composite nanocoatings for dental metals, and the promotion of the environmentally friendly large-scale production of high-quality multifunctional GFN-based nanocoatings in the field of oral science.

Biomechanical analysis of effective mandibular en-masse retraction using Class II elastics with a clear aligner: a finite element study.

BACKGROUND: This study aimed to evaluate the displacement and stress distribution of mandibular dentition by various positions of the Class II elastics during en-masse retraction in clear aligner therapy. METHODS: Models including a mandibular dentition (without first premolars), periodontal ligament (PDL), mandible, as well as attachments, aligners and buttons were constructed and imported into Ansys Workbench 2019 (ANSYS, USA) to generate the three-dimensional (3D) finite element model. Six combinations were created: (1) aligner alone (control), (2)-(5) Class II elastics with buttons placed on the mesiobuccal (MB), distobuccal (DB), mesiolingual (ML) and distolingual (DL) surface of the mandibular first molar, and (6) Class II elastics with a button on the aligner corresponding to the mesiobuccal surface of the mandibular first molar (AMB). The elastic force was set to 2 N for simulations. RESULTS: The central incisors appeared lingual tipping in the six models. The lingual crown movement of the central incisors was 0.039 mm, 0.034 mm, 0.034 mm, 0.042 mm, 0.041 mm, and 0.034 mm for control model, MB model, DB model, ML model, DL model, and AMB model, respectively. The first molars showed mesial tipping in the six models. The mesial movement of the mesiobuccal cusps of the first molars was 0.045 mm, 0.060 mm, 0.063 mm, 0.048 mm, 0.051 mm, and 0.055 mm for control model, MB model, DB model, ML model, DL model, and AMB model, respectively. CONCLUSIONS: Class II elastics reduced lingual tipping of anterior teeth but aggravated mesial tipping of posterior teeth. Mesiolingual elastics developed minimum mesial tipping of the posterior teeth. When Class II elastics are required, attaching elastics on the mesiolingual surface of the mandibular first molar is recommended to prevent mandibular anchorage loss.

Biological Effects, Applications and Design Strategies of Medical Polyurethanes Modified by Nanomaterials.

Polyurethane (PU) has wide application and popularity as medical apparatus due to its unique structural properties relationship. However, there are still some problems with medical PUs, such as a lack of functionality, insufficient long-term implantation safety, undesired stability, etc. With the rapid development of nanotechnology, the nanomodification of medical PU provides new solutions to these clinical problems. The introduction of nanomaterials could optimize the biocompatibility, antibacterial effect, mechanical strength, and degradation of PUs via blending or surface modification, therefore expanding the application range of medical PUs. This review summarizes the current applications of nano-modified medical PUs in diverse fields. Furthermore, the underlying mechanisms in efficiency optimization are analyzed in terms of the enhanced biological and mechanical properties critical for medical use. We also conclude the preparation schemes and related parameters of nano-modified medical PUs, with discussions about the limitations and prospects. This review indicates the current status of nano-modified medical PUs and contributes to inspiring novel and appropriate designing of PUs for desired clinical requirements.

Astrocyte responses to nanomaterials: Functional changes, pathological changes and potential applications.

Astrocytes are responsible for regulating and optimizing the functional environment of neurons in the brain and can reduce the adverse impacts of external factors by protecting neurons. However, excessive astrocyte activation upon stimulation may alter their initial protective effect and actually lead to aggravation of injury. Similar to the dual effects of astrocytes in the response to injury within the central nervous system (CNS), nanomaterials (NMs) can have either toxic or beneficial effects on astrocytes, serving to promote injury or inhibit tumors. As the important physiological functions of astrocytes have been gradually revealed, the effects of NMs on astrocytes and the underlying mechanisms have become a new frontier in nanomedicine and neuroscience. This review summarizes the in vitro and in vivo findings regarding the effects of various NMs on astrocytes, focusing on functional alterations and pathological processes in astrocytes, as well as the possible underlying mechanisms. We also emphasize the importance of co-culture models in studying the interaction between NMs and cells of the CNS. Finally, we discuss NMs that have shown promise for application in astrocyte-related diseases and propose some challenges and suggestions for further investigations, with the aim of providing guidance for the widespread application of NMs in the CNS.

Corrosion Behavior and In Vitro Cytotoxicity of Ni-Ti and Stainless Steel Arch Wires Exposed to Lysozyme, Ovalbumin, and Bovine Serum Albumin.

In this study, the tendency and mechanisms by which protein and mechanical loads contribute to corrosion were determined by exposing Ni-Ti and stainless steel arch wires under varying mechanical loads to artificial saliva containing different types of protein (lysozyme, ovalbumin, and bovine serum albumin). The corrosion behavior and in vitro cytotoxicity results show that exposure to both protein and mechanical stress significantly decreased the corrosion resistance of stainless steel and increased the release of toxic corrosion products. Adding protein inhibited the corrosion of Ni-Ti, but the mechanical loads counteracted this effect. Even proteins containing the same types of amino acids had different effects on the corrosion resistance of the same alloy. The effect of protein or stress, or their combination, should be considered in the application of metal medical materials.