[Community structure of phyllosphere fungi associated with dominant tree species in a broad-leaved Korean pine forest of Changbai Mountain, Northeast China]. / é•¿ç™½å±±é˜”å¶çº¢æ¾æž—ä¼˜åŠ¿æ ‘ç§å¶é™ çœŸèŒç¾¤è½ç»“æž„.

Forest is the main component of terrestrial ecosystems that harbors about 40% of the existing species on the earth. As a vital component of biodiversity, phyllosphere microbes in the canopy play a critical and unique role in maintaining plant health, improving host resistance, and influencing global biogeochemical cycle. However, the studies on the community structure of phyllosphere fungi in natural forests are scarce as compared to that on rhizosphere microbes. Consequently, we know litter about how phyllosphere fungi associates with leaf traits. In this study, we analyzed fungal community composition of canopy leaves of six dominant tree species (i.e., Pinus koraiensis, Tilia amurensis, Quercus mongolica, Acer mono, Fraxinus mandshurica, and Ulmus japonica), in a broad-leaved Korean pine forest of Changbai Mountain Nature Reserve in Jilin Province, using high-throughput sequencing. We compared the differences of phyllosphere fungal community structure and functional groups of different dominant tree species. Moreover, 14 key leaf functional traits of their host trees were measured to investigate the relationships between fungal community composition and leaf functional traits. We found that the dominant phyla and class of phyllosphere fungi were Ascomycota and Basidiomycota, and Dothideomycetes and Taphrinomycetes, respectively. Results of LEfSe analysis indicated that all the tree species except Ulmus japonica had significant biomarkers, such as the Eurotiomycetes of Pinus koraiensis and the Ascomycetes of Quercus mongolica. The main functional groups of phyllosphere fungi were pathotroph. The results of redundancy and envfit analysis showed that functional traits related to plant nutrient acquisition as well as resistance to diseases and pests were the main factors influencing the community structure of phyllosphere fungi.

[Variation characteristics of NDVI and its response to climatic change in the growing season of Changbai Mountain Nature Reserve during 2001 and 2018]. / 2001­2018年长白山自然保护区生长季NDVIå˜åŒ–ç‰¹å¾åŠå ¶å¯¹æ°”å€™å˜åŒ–çš„å“åº”.

To understand the dynamics of temperate forest in Northeast Asia and its response to climate change under the scenario of global change, we examined the temporal and spatial changes of normalized difference vegetation index (NDVI) and their correlation with temperature and precipitation of Changbai Mountain Nature Reserve in the growing season during 2001 and 2018, based on the remote sensing database of MODIS with a resolution of 250 m, land surface temperature data with a resolution of 1 km and meteorological data in the studied and surrounding area. The results showed that, in the growing season of 2001-2018, the averaged NDVI value of the study area was 0.711. Vegetation coverage was relatively high, increasing with a rate of 0.0025·a-1. The temperature showed an extremely significantly increasing trend (0.032 ℃·a-1), the rate of which was higher than that at global level. Precipitation also showed a significantly increasing trend (5.54 mm·a-1) with increased interannual variation. Spatially, NDVI generally was higher in the northwest and decreased with elevation. During the study period, the area with increased NDVI accounted for 46.2%, mainly concentrated in the north and south central high-altitude areas, while 53.8% of total area remained unchanged or slightly decrease. NDVI of the study area was mainly affected by temperature. At the annual scale, NDVI and land surface temperature were positively correlated, with 90.2% presented positive correlation and 43.6% significantly correlated. At the monthly scale, the impact of temperature on NDVI was more significant at the beginning and the end of growing season.

[Seasonal variation of water-soluble ions in PM2.5 at Changbai Mountain].

To study seasonal variation of water-soluble ions in PM2.5 at Changbai Mountain. PM2.5 was collected with a high-volume sampler from Jun. 2005 to Dec. 2008, and the concentrations of water-soluble ions were analyzed using ion chromatography. The results showed that the three major ions of SO4(2-), NH4+ and NO3 showed obvious seasonal variation. The mass concentration of SO4(2-) was the highest in summer and lowest in autumn. The mass concentration of NO3- was the highest in winter and lowest in summer. The seasonal variation of NH4+ was influenced by SO4(2-) and NO3-. The total concentrations of water-soluble ions in PM2.5 from different directions were evidently different, following the order of NE < NW < SW, with the average concentrations of 5.43, 7.63 and 10.26 microg x m(-3), respectively. Ca2+ was strongly correlated with CO3(2-), and the correlation(R) was higher in spring (0.74) than that in summer (0.30).

Needle-age related variability in nitrogen, mobile carbohydrates, and δ13C within Pinus koraiensis tree crowns.

For both ecologists and physiologists, foliar physioecology as a function of spatially and temporally variable environmental factors such as sunlight exposure within a tree crown is important for understanding whole tree physiology and for predicting ecosystem carbon balance and productivity. Hence, we studied concentrations of nitrogen (N), non-structural carbohydrates (NSC = soluble sugars + starch), and δ(13)C in different-aged needles within Pinus koraiensis tree crowns, to understand the needle age- and crown position-related physiology, in order to test the hypothesis that concentrations of N, NSC, and δ(13)C are needle-age and crown position dependent (more light, more photosynthesis affecting N, NSC, and δ(13)C), and to develop an accurate sampling strategy. The present study indicated that the 1-yr-old needles had significantly higher concentration levels of mobile carbohydrates (both on a mass and an area basis) and N(area) (on an area basis), as well as NSC-N ratios, but significantly lower levels of N(mass) (on a mass basis) concentration and specific leaf area (SLA), compared to the current-year needles. Azimuthal (south-facing vs. north-facing crown side) effects were found to be significant on starch [both on a mass (ST(mass)) and an area basis (ST(area))], δ(13)C values, and N(area), with higher levels in needles on the S-facing crown side than the N-facing crown side. Needle N(mass) concentrations significantly decreased but needle ST(mass), ST(area), and δ(13)C values significantly increased with increasing vertical crown levels. Our results suggest that the sun-exposed crown position related to photosynthetic activity and water availability affects starch accumulation and carbon isotope discrimination. Needle age associated with physiological activity plays an important role in determining carbon and nitrogen physiology. The present study indicates that across-scale sampling needs to carefully select tissue samples with equal age from a comparable crown position.