Complete mitochondrial genome of the golden-crowned sifaka, Propithecus tattersalli (Primates: Indriidae).

The golden-crowned sifaka, Propithecus tattersalli, is a critically endangered social lemur species that inhabits the restricted and highly fragmented semi-evergreen forests. In this study, we determined the complete mitochondrial genome sequence of this species for the first time. The results shows that this mtDNA genome is 17 099 bp in size, and comprises 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes, and one control region (D-loop). The overall base composition in descending order is A (32.91%), C (27.07%), T (27.04%), and G (12.98%), so the percentage of A and T (59.95%) is slightly higher than that of G and C. The gene order and the composition of P. tattersalli mitochondrial genome are exactly similar to those of most other vertebrates. All the genes are encoded on the heavy strand with the exception of NADH dehydrogenase subunit six (ND6) and eight tRNA genes. Phylogenetic analysis suggests that the P. tattersalli exhibits most close relationship with P. coquereli. The complete mitochondrial genome sequence presented here will be useful for further phylogenetic analysis and conservation genetic studies in P. tattersalli.

[Study on the levels of DA and metabolite in striatum in rats with Parkinson's disease treated by BDNF gene modified bone mesenchymal stem cells].

OBJECTIVE: To investigate the effects of dopamine (DA) and metabolite in striatum of Parkinson's disease (PD) rats treated by bone mesenchymal stem cells (MSCs) modified by plasmid pIRESneo-EGFP-BDNF. METHOD: pIRESneo-EGFP-BDNF was transfected to MSCs with electroporation. The rat models of PD were set up by 6-OHDA and then divided into four groups randomly, which were Sham group, PD group, BDNF group. The rotating behavior of rat models induced by apomorphine (APO) intraperitoneally which transplanting bone MSCs or MSCs modified by plasmid pIRESneo-EGFP-BDNF through cerebral lateral ventricle after 2, 4 and 8 weeks. The levels of DA, homovanillic acid (HVA), dihydroxy-phenylacetic acid (DOPAC) were measured by high performance liquid chromatography (HPLC) in striatum of each group. RESULTS: The rotation numbers (r/min) of MSCs group or BDNF group in the 2nd, 4th and 8th week after transplanting were significantly decreased compared with that of PD group (P < 0.05). Those of BDNF group were specially significant compared with those of MSCs group (P < 0.05). The levels of DA, HVA, DOPAC and the ratios of DA/HVA, DA/DOPAC in stratum after PD rats intervened by transplanting cells through cerebral lateral ventricle after eight weeks were increased significantly in BDNF group or MSCs group while compared with PD group, especially in BDNF group. CONCLUSION: The behavior of rat with PD was improved significantly by increasing the levels of DA and decreasing metabolic rate of DA in striatum while transplanting BDNF modified bone MSCs through cerebral lateral ventricle.