RESUMO
In order to investigate the influence of different embedding methods on the properties and function of polyphenols, the Epigallocatechin gallate (EGCG) loaded chitosan nanoparticles prepared with or without ß-lactoglobulin (ß-Lg) were obtained by ionic cross linking method. The average particle sizes of EGCG loaded chitosan nanoparticles (EGCG-CS NPs) decreased from 190 nm to 157 nm after adding with ß-Lg, whereas the encapsulation efficiency (EE) increased from 59.79 % to 76.29 %. The results of transmission electron microscopy (TEM) showed that the obtained nanoparticles had obvious core-shell structure. The results of simulated gastrointestinal digestion showed that the release rate of EGCG in CS/ß-Lg NPs was much lower than that of CS-NPs. Compared with free EGCG, the DPPH and FRAP assay showed that EGCG-CS NPs and EGCG-CS/ß-Lg NPs had slow-controlled antioxidant activity. Meanwhile, the study of cellular antioxidant activity (CAA) showed that the EC50 values of EGCG-CS NPs and EGCG-CS/ß-Lg NPs were decreased by 8.56 % and 18.35 %, respectively.
Assuntos
Antioxidantes/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Catequina/análogos & derivados , Quitosana/farmacologia , Lactoglobulinas/farmacologia , Nanopartículas/química , Picratos/antagonistas & inibidores , Antioxidantes/síntese química , Antioxidantes/química , Células CACO-2 , Catequina/química , Catequina/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Quitosana/química , Humanos , Lactoglobulinas/química , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
Antioxidant edible films based on chitosan hydrochloride (CHC) and epigallocatechin gallate (EGCG)-loaded nanocapsules (NCs) were developed. The CHC films incorporated with NCs were prepared by a casting method in three different proportions. The obtained films were characterized using the techniques including scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. Meanwhile, the mechanical and color properties, optical transmittance, EGCG release profile and antioxidant activity were also determined. The addition of NCs to CHC films increased their tensile strength (TS) and the percent elongation at break (%E). Lightness was significantly decreased in the NCs embedded films compared to controls, which in turn led to high antioxidant activity and excellent barrier properties against visible light. EGCG was rapidly released into food simulant although the concentration fluctuates over time. Therefore, these films can prevent the oxidation of fatty food stuffs.