La2O3 Nanoparticles Can Cause Cracking of Tomato Fruit through Genetic Reconstruction.

La2O3 nanoparticles (NPs) have shown great potential in agriculture, but cracking of plant sensitive tissue could occur during application, resulting in a poor appearance, facilitating entry for insects and fungi, and increasing economic losses. Herein, exocarp cracking mechanisms of tomato (Solanum lycopersicum L.) fruit in response to La2O3 NPs were investigated. Tomato plants were exposed to La2O3 NPs (0-40 mg/L, 90 days) by a split-root system under greenhouse condition. La2O3 NPs with high concentrations (25 and 40 mg/L) increased the obvious cracking of the fruit exocarp by 20.0 and 22.7%, respectively. After exposure to 25 mg/L La2O3 NPs, decreased thickness of the cuticle and cell wall and lower wax crystallization patterns of tomato fruit exocarp were observed. Biomechanical properties (e.g., firmness and stiffness) of fruit exocarp were decreased by 34.7 and 25.9%, respectively. RNA-sequencing revealed that the thinner cuticle was caused by the downregulation of cuticle biosynthesis related genes; pectin remodeling, including the reduction in homogalacturonan (e.g., LOC101264880) and rhamnose (e.g., LOC101248505), was responsible for the thinner cell wall. Additionally, genes related to water and abscisic acid homeostasis were significantly upregulated, causing the increases of water and soluble solid content of fruit and elevated fruit inner pressure. Therefore, the thinner fruit cuticle and cell wall combined with the higher inner pressure caused fruit cracking. This study improves our understanding of nanomaterials on important agricultural crops, including the structural reconstruction of fruit exocarp contributing to NPs-induced cracking at the molecular level.

Microplastic fragmentation by rotifers in aquatic ecosystems contributes to global nanoplastic pollution.

The role of aquatic organisms in the biological fragmentation of microplastics and their contribution to global nanoplastic pollution are poorly understood. Here we present a biological fragmentation pathway that generates nanoplastics during the ingestion of microplastics by rotifers, a commonly found and globally distributed surface water zooplankton relevant for nutrient recycling. Both marine and freshwater rotifers could rapidly grind polystyrene, polyethylene and photo-aged microplastics, thus releasing smaller particulates during ingestion. Nanoindentation studies of the trophi of the rotifer chitinous mastax revealed a Young's modulus of 1.46 GPa, which was higher than the 0.79 GPa for polystyrene microparticles, suggesting a fragmentation mechanism through grinding the edges of microplastics. Marine and freshwater rotifers generated over 3.48 × 105 and 3.66 × 105 submicrometre particles per rotifer in a day, respectively, from photo-aged microplastics. Our data suggest the ubiquitous occurrence of microplastic fragmentation by different rotifer species in natural aquatic environments of both primary and secondary microplastics of various polymer compositions and provide previously unidentified insights into the fate of microplastics and the source of nanoplastics in global surface waters.

Heterogeneous aggregation of carbon and silicon nanoparticles with benzo[a]pyrene modulates their impacts on the pulmonary surfactant film.

Inhaled nanoparticles (NPs) can deposit in alveoli where they interact with the pulmonary surfactant (PS) and potentially induce toxicity. Although nano-bio interactions are influenced by the physicochemical properties of NPs, isolated NPs used in previous studies cannot accurately represent those found in atmosphere. Here we used molecular dynamics simulations to investigate the interplay between two types of NPs associated with benzo[a]pyrene (BaP) at the PS film. Silicon NPs (SiNPs), regardless of aggregation and adsorption, directly penetrated through the PS film with minimal disturbance. Meanwhile, BaPs adsorbed on SiNPs were rapidly solubilized by PS, increasing the BaP's bioaccessibility in alveoli. Carbon NPs (CNPs) showed aggregation and adsorption-dependent effects on the PS film. Compared to isolated CNPs, which extracted PS to form biomolecular coronas, aggregated CNPs caused more pronounced PS disruption, especially around irregularly shaped edges. SiNPs in mixture exacerbated the PS perturbation by piercing PS film around the site of CNP interactions. BaPs adsorbed on CNPs were less solubilized and suppressed PS extraction, but aggravated biophysical inhibition by prompting film collapse under compression. These results suggest that for proper assessment of inhalation toxicity of airborne NPs, it is imperative to consider their heterogeneous aggregation and adsorption of pollutants under atmospheric conditions.

Detection, distribution and environmental risk of metal-based nanoparticles in a coastal bay.

Metal-based nanoparticles (NPs) attract increasing concerns because of their adverse effects on aquatic ecosystems. However, their environmental concentrations and size distributions are largely unknown, especially in marine environments. In this work, environmental concentrations and risks of metal-based NPs were examined in Laizhou Bay (China) using single-particle inductively coupled plasma-mass spectrometry (sp-ICP-MS). First, separation and detection approaches of metal-based NPs were optimized for seawater and sediment samples with high recoveries of 96.7% and 76.3%, respectively. Spatial distribution results showed that Ti-based NPs had the highest average concentrations for all the 24 stations (seawater, 1.78 × 108 particles/L; sediments, 7.75 × 1012 particles/kg), followed by Zn-, Ag-, Cu-, and Au-based NPs. For all the NPs in seawater, the highest abundance occurred around the Yellow River Estuary, resulting from a huge input from Yellow River. In addition, the sizes of metal-based NPs were generally smaller in sediments than those in seawater (22, 20, 17, and 16 of 22 stations for Ag-, Cu-, Ti-, and Zn-based NPs, respectively). Based on the toxicological data of engineered NPs, predicted no-effect concentrations (PNECs) to marine species were calculated as Ag at 72.8 ng/L < ZnO at 2.66 µg/L < CuO at 7.83 µg/L < TiO2 at 72.0 µg/L, and the actual PNECs of the detected metal-based NPs may be higher due to the possible presence of natural NPs. Station 2 (around the Yellow River Estuary) was assessed as "high risk" for Ag- and Ti-based NPs with risk characterization ratio (RCR) values of 1.73 and 1.66, respectively. In addition, RCRtotal values for all the four metal-based NPs were calculated to fully assess the co-exposure environmental risk, with 1, 20, and 1 of 22 stations as "high risk", "medium risk", and "low risk", respectively. This study helps to better understand the risks of metal-based NPs in marine environments.

Molecular assembly of extracellular polymeric substances regulating aggregation of differently charged nanoplastics and subsequent interactions with bacterial membrane.

Extracellular polymeric substances (EPS) represent an interface between microbial cells and aquatic environment, where nanoplastics acquire coatings to alter their fate and toxicity. However, little is known about molecular interactions governing modification of nanoplastics at biological interfaces. Molecular dynamics simulations combining experiments were conducted to investigate assembly of EPS and its regulatory roles in the aggregation of differently charged nanoplastics and interactions with bacterial membrane. Driven by hydrophobic and electrostatic interactions, EPS formed micelle-like supramolecular structures with hydrophobic core and amphiphilic exterior. Different components, depending on their hydrophobicity and charge, were found to promote or suppress EPS assembly. Neutral and hydrophobic nanoplastics showed unbiased adsorption of EPS species, while cationic and anionic nanoplastics were distinct and attracted specific molecules of opposite charges. Compared with isolated EPS, assembled EPS concealed hydrophobic groups to be less adsorbed by nanoplastics. Aggregation of nanoplastics was alleviated by EPS due to electrostatic repulsion plus steric hindrance. ESP suppressed binding of cationic nanoplastics to the bacterial membrane through reducing the surface charge. Neutral and anionic nanoplastics showed weak membrane association, but their binding interactions were promoted by EPS. The structural details revealed here provided molecular level insights into modifications of nanoplastics at the eco-environment interface.

Coastal phytoplankton blooms expand and intensify in the 21st century.

Phytoplankton blooms in coastal oceans can be beneficial to coastal fisheries production and ecosystem function, but can also cause major environmental problems1,2-yet detailed characterizations of bloom incidence and distribution are not available worldwide. Here we map daily marine coastal algal blooms between 2003 and 2020 using global satellite observations at 1-km spatial resolution. We found that algal blooms occurred in 126 out of the 153 coastal countries examined. Globally, the spatial extent (+13.2%) and frequency (+59.2%) of blooms increased significantly (P < 0.05) over the study period, whereas blooms weakened in tropical and subtropical areas of the Northern Hemisphere. We documented the relationship between the bloom trends and ocean circulation, and identified the stimulatory effects of recent increases in sea surface temperature. Our compilation of daily mapped coastal phytoplankton blooms provides the basis for global assessments of bloom risks and benefits, and for the formulation or evaluation of management or policy actions.

Sediment-seawater exchange altered adverse effects of ocean acidification towards marine microalgae.

Ocean acidification (OA) exhibits high threat to marine microalgae. However, the role of marine sediment in the OA-induced adverse effect towards microalgae is largely unknown. In this work, the effects of OA (pH 7.50) on the growth of individual and co-cultured microalgae (Emiliania huxleyi, Isochrysis galbana, Chlorella vulgaris, Phaeodactylum tricornutum, and Platymonas helgolandica tsingtaoensis) were systematically investigated in the sediment-seawater systems. OA inhibited E. huxleyi growth by 25.21 %, promoted P. helgolandica (tsingtaoensis) growth by 15.49 %, while did not cause any effect on the other three microalgal species in the absence of sediment. In the presence of the sediment, OA-induced growth inhibition of E. huxleyi was significantly mitigated, because the released chemicals (N, P and Fe) from seawater-sediment interface increased the photosynthesis and reduced oxidative stress. For P. tricornutum, C. vulgaris and P. helgolandica (tsingtaoensis), the growth was significantly increased in the presence of sediment in comparison with those under OA alone or normal seawater (pH 8.10). For I. galbana, the growth was inhibited when the sediment was introduced. Additionally, in the co-culturing system, C. vulgaris and P. tricornutum were the dominant species, while OA increased the proportions of dominant species and decreased the community stability as indicated by Shannon and Pielou's indexes. After the introduction of sediment, the community stability was recovered, but remained lower than that under normal condition. This work demonstrated the role of sediment in the biological responses to OA, and could be helpful for better understanding the impact of OA on marine ecosystems.

Transfer of CeO2 nanoparticles between freshwater omnivorous organisms: Effect of feces and necrophagy.

Transfer of CeO2 engineered nanoparticles (NPs) through feces was investigated between two omnivorous organisms, red crucian carp (Carassius auratus red var.) and crayfish (Procambarus clarkii). Upon water exposure (5 mg/L, 7 days), the highest bioaccumulation was observed in carp gills (5.95 µg Ce/g D.W.) and crayfish hepatopancreas (648 µg Ce/g D.W.), with the bioconcentration factors (BCFs) at 0.45 and 3.61, respectively. In addition, 97.4% and 73.0% of ingested Ce were excreted by carp and crayfish, respectively. The feces of carp and crayfish were collected and fed to crayfish and carp, respectively. After feces exposure, bioconcentration was observed in both carp (BCF, 3.00) and crayfish (BCF, 4.56). After feeding crayfish with carp bodies (1.85 µg Ce/g D.W.), CeO2 NPs were not biomagnified (biomagnification factor, 0.28). Upon water exposure, CeO2 NPs were transformed into Ce(III) in the feces of both carp (24.6%) and crayfish (13.6%), and the transformation was stronger after subsequent feces exposure (100% and 73.7%, respectively). Feces exposure lowered histopathological damage, oxidative stress, and nutritional quality (e.g., crude proteins, microelements, amino acids) to carp and crayfish in comparison with water exposure. This research highlights the importance of feces exposure on the transfer and fate of NPs in aquatic ecosystems.

Antagonism toxicity of CuO nanoparticles and mild ocean acidification to marine algae.

The toxicity of CuO nanoparticles (NPs) to marine microalgae (Emiliania huxleyi) under ocean acidification (OA) conditions (pHs 8.10, 7.90, 7.50) was investigated. CuO NPs (5.0 mg/L) caused significant toxicity (e.g., 48-h growth inhibition, 20%) under normal pH (8.10), and severe OA (pH 7.50) increased the toxicity of CuO NPs (e.g., 48-h growth inhibition, 68%). However, toxicity antagonism was observed with a growth inhibition (48 h) decreased to 37% after co-exposure to CuO NPs and mild OA (pH 7.90), which was attributed to the released Cu2+ ions from CuO NPs. Based on biological responses as obtained from RNA-sequencing, the dissolved Cu2+ ions (0.078 mg/L) under mild OA were found to increase algae division (by 17%) and photosynthesis (by 28%) through accelerating photosynthetic electron transport and promoting ATP synthesis. In addition, mild OA enhanced EPS secretion by 41% and further increased bioavailable Cu2+ ions, thus mitigating OA-induced toxicity. In addition, excess Cu2+ ions could be transformed into less toxic Cu2S and Cu2O based on X-ray absorption near-edge spectroscopy (XANES) and high-resolution transmission electron microscopy (HR-TEM), which could additionally regulate the antagonism effect of CuO NPs and mild OA. The information advances our knowledge in nanotoxicity to marine organisms under global climate change.

Foliar Application of Reaction Products Derived from Selenite Removal by Iron Monosulfide for Brassica rapa ssp. Chinensis L.

The extensive application of FeS in environmental remediation requires the recovery and reuse of reaction products between FeS and pollutants. Therefore, foliar application of reaction products derived from selenite [Se(IV)] removal by FeS for pak choi was performed. The removal rate of Se(IV) by 100 mg/L FeS was 0.047 h-1. 93.2% of Se(IV) was reduced to Se(0), and FeS was correspondingly oxidized to goethite (78.9%), lepidocrocite (21.1%), and S(0) (91.5%) based on the analysis of X-ray absorption fine structure. The reaction products promoted the growth of pak choi in terms of fresh biomass, vitamin C, and protein, ascribed to the key roles of Fe and S in enhancing the electron transfer rate and light conversion rate. Furthermore, the application of reaction products decreased by 64% of disease incidence as compared with the pathogen Pseudomonas syringae pv. maculicola-infected control. The total Se content in plants increased to 576 µg/kg and was composed of 11.9% of SeMeCys, 29.8% of SeMet, and 58.3% of SeCys after exposure to reaction products, which is beneficial to the human dietary intake from pak choi. This study demonstrated that the reaction products between FeS and Se(IV) could be recovered and applied as a nano-enabled strategy to prevent crop insecurity.

Physiological and proteomic analyses reveal the effect of CeO2 nanoparticles on strawberry reproductive system and fruit quality.

With the rapid development of nanotechnology, the environmental impact assessment of nanoparticles (NPs) becomes a pressing problem to ensure the environmental safety and human health. However, the roles of CeO2 NPs on the development of crop reproductive system and fruit quality are largely unknown. Herein, strawberry (Fragaria × ananassa Duch.) plants were exposed to CeO2 NPs at 0-115 mg/L. The reduced generation of stolon, increased pollen numbers and germination rate of pollen grains, as well as the elongation of pollen tubes contributed to the enhanced yield of strawberry upon CeO2 NP exposure (6-115 mg/L). Furthermore, the fruit quality was improved by increasing total phenols, vitamin C, amino acids, and protein contents. More importantly, even at 115 mg/L treatment, fruit Ce contents (4.4 mg/kg) did not exceed the Ce mean oral reference dose of humans. Finally, label-free proteomic results disclosed that differentially expressed proteins related to malate were down-regulated, and the differential regulation of sugar-associated proteins suggested the changes in sugar composition. The significantly different expression of ascorbate and glutathione related proteins exhibited the increased antioxidant capacity of fruits. The findings in the present study provide new perspectives for understanding the potential risk posed by NPs in the environment.

Adsorption and catalytic degradation of preservative parabens by graphene-family nanomaterials.

Parabens pose increasing threats to human health due to endocrine disruption activity. Adsorption and degradation of parabens by three types of graphene-family nanomaterials (GFNs) were therefore investigated. For a given paraben, the maximum adsorption capacities (Q0) followed the order of reduced graphene oxide (RGO) > multilayered graphene (MG) > graphene oxide (GO); for a given GFN, Q0 followed the order of butylparaben (BuP) > propylparaben (PrP) > ethylparaben (EtP) > methylparaben (MeP), dominated by hydrophobic interaction. MeP removal by all the three GFNs was highly enhanced (0.55-4.37 times) with the assistance of H2O2 due to additional catalytic degradation process, and MG showed the highest removal enhancement. ∙OH was confirmed as the dominant radicals responsible for parabens degradation. For MG and RGO, the metal impurities (Fe, Cu, Mn, and Co) initiated Fenton-like reaction with H2O2 to generate ∙OH. GO contained oxygen-centered free radicals, which were responsible for ∙OH formation via transferring electron to H2O2. Four degradation byproducts of MeP were identified, including oxalic, propanedioic, fumaric, and 2,5-dihydroxybenzoic acids. Combined with density function theory calculations, the degradation sites and pathways were identified and confirmed. These findings provide useful information on mechanistic understanding towards the adsorption and degradation of parabens by GFNs.

Benzo[a]pyrene and heavy metal ion adsorption on nanoplastics regulated by humic acid: Cooperation/competition mechanisms revealed by molecular dynamics simulations.

Nanoplastics adsorb pollutants and organic matter to aggravate or alleviate impact to the eco-environment and human health. However, the interaction mechanisms remain unclear and difficult to study using current experimental techniques. By means of molecular dynamics simulation, here we investigate adsorption of benzo[a]pyrene (BaP) and heavy metal ions (Cu2+) on nanoplastics of different materials and surface charges regulated by humic acid (HA). Among considered materials, polystyrene shows the highest capacity of adsorbing BaPs via forming sandwiched π-stacking structures with benzene rings. Driven by hydrophobic, electrostatic and hydrogen bonding interactions, HAs spontaneously aggregate into micelle-like structures with hydrophobic core and charged exterior accessible to BaPs and Cu2+, respectively. Cationic and neutral nanoplastics adsorb more HAs to form eco-coronas, which modulate BaP and Cu2+ adsorption via following cooperation/competition mechanisms. On one hand, the direct binding of BaPs to nanoplastics is hindered by HAs through BaP encapsulation plus competitive adsorption. On the other hand, adsorbed HAs expose carboxyl groups to offer rich binding sites to promote Cu2+ adsorption on neutral and cationic nanoplastics, while unbound HAs compete with anionic nanoplastics to inhibit Cu2+ adsorption. These results provide molecular level insights into transport, transformation and accessibility of nanoplastics with coexisting contaminants in the aqueous environment.

Molecular modeling of nanoplastic transformations in alveolar fluid and impacts on the lung surfactant film.

Airborne nanoplastics can be inhaled to threaten human health, but research on the inhaled nanoplastic toxicity is in its infancy, and interaction mechanisms are largely unknown. By means of molecular dynamics simulation, we employed spherical nanoplastics of different materials and aging properties to predict and elucidate nanoplastic transformations in alveolar fluid and impacts on the lung surfactant (LS) film at the alveolar air-water interface. Results showed spontaneous adsorption of LS molecules on nanoplastics of 10 nm in diameter, and the adsorption layer can be defined as coronas, which increased the particle size, reduced and equalized the surface hydrophobicity, and endowed nanoplastics with negative surface charges. Nanoplastics of polypropylene and polyvinylchloride materials were dissolved by LS, which could increase bioavailability of polymers and toxic additives. Aging properties represented by the nanoplastic size, polymer's molecular weight and surface chemistry altered nanoplastic transformations through modulating competition between polymer-LS and polymer-polymer interactions. Upon transferred to the alveolar air-water interface through vesicle fusion, nanoplastics could interfere with the normal biophysical function of LS through disrupting the LS ultrastructure and fluidity, and prompting collapse of the LS film. These results provide new molecular level insights into fate and toxicity of airborne nanoplastics in human respiratory system.

Competitive and/or cooperative interactions of graphene-family materials and benzo[a]pyrene with pulmonary surfactant: a computational and experimental study.

BACKGROUND: Airborne nanoparticles can be inhaled and deposit in human alveoli, where pulmonary surfactant (PS) molecules lining at the alveolar air-water interface act as the first barrier against inhaled nanoparticles entering the body. Although considerable efforts have been devoted to elucidate the mechanisms underlying nanoparticle-PS interactions, our understanding on this important issue is limited due to the high complexity of the atmosphere, in which nanoparticles are believed to experience transformations that remarkably change the nanoparticles' surface properties and states. By contrast with bare nanoparticles that have been extensively studied, relatively little is known about the interactions between PS and inhaled nanoparticles which already adsorb contaminants. In this combined experimental and computational effort, we investigate the joint interactions between PS and graphene-family materials (GFMs) with coexisting benzo[a]pyrene (BaP). RESULTS: Depending on the BaP concentration, molecular agglomeration, and graphene oxidation, different nanocomposite structures are formed via BaPs adsorption on GFMs. Upon deposition of GFMs carrying BaPs at the pulmonary surfactant (PS) layer, competition and cooperation of interactions between different components determines the interfacial processes including BaP solubilization, GFM translocation and PS perturbation. Importantly, BaPs adsorbed on GFMs are solubilized to increase BaP's bioavailability. By contrast with graphene adhering on the PS layer to release part of adsorbed BaPs, more BaPs are released from graphene oxide, which induces a hydrophilic pore in the PS layer and shows adverse effect on the PS biophysical function. Translocation of graphene across the PS layer is facilitated by BaP adsorption through segregating it from contact with PS, while translocation of graphene oxide is suppressed by BaP adsorption due to the increase of surface hydrophobicity. Graphene extracts PS molecules from the layer, and the resultant PS depletion declines with graphene oxidation and BaP adsorption. CONCLUSION: GFMs showed high adsorption capacity towards BaPs to form nanocomposites. Upon deposition of GFMs carrying BaPs at the alveolar air-water interface covered by a thin PS layer, the interactions of GFM-PS, GFM-BaP and BaP-PS determined the interfacial processes of BaP solubilization, GFM translocation and PS perturbation.

Comparison of the energetic, environmental, and economic performances of three household-based modern bioenergy utilization systems in China.

The methods of life cycle assessment and cost-effectiveness analysis were employed to compare the energetic, environmental, and economic performances of three household-based modern bioenergy (MBE) utilization systems: biogas utilization (BGs), biomass briquette utilization for energy only (BBs), and biomass briquette utilization for energy and biochar (BCs). The results showed that all the three MBEs performed positive results in net energy output, which ranged from 0.86 to 3.75 MJ (kg crop residues)-1. The positive greenhouse gas reductions also can be acquired when using these three MBEs to substitute for traditional energy, respectively. The cost-effectiveness values of three MBE systems ranged from 343 RMB (tCO2e)-1 for BGs to 598 RMB (tCO2e)-1 for BCs (the CO2e indicates the CO2 equivalent). Considering the supply index analysis results furtherly, the BGs is regarded as the best strategy for the development of MBE in rural households. However, BG leakage during BG digester operations should be received much greater attention to avoid adverse impacts on GHG mitigation.

Combined effects of biochar properties and soil conditions on plant growth: A meta-analysis.

Biochar application in agricultural soils can be highly beneficial to plant productivity. However, how plant productivity response (PPR) [% change of plant yield from control (without biochar application)] to biochar application is affected by biochar properties, soil conditions, and their combinations is still unclear. Therefore, a meta-analysis based on 1254 paired comparisons from 153 published studies was conducted. The grand mean of PPR was estimated to be 16.0 ± 1.3%, regardless of biochar/soil conditions. Meanwhile, a large variation of PPR from -31.8% to 974% was also observed under different biochar or/and soil conditions. Specifically, biochar properties including pH, cation exchange capacity (CEC), contents of carbon and ash, bulk density, and soil conditions including texture, pH, CEC, nitrogen content, and C/N ratio significantly affected the results of PPR to biochar addition. Furthermore, the liming effect, improvement in soil physical structure, and increased nutrient use efficiency were suggested as the key mechanisms for the positive PPR in biochar-amended soils. Moreover, PPR could be significantly affected (strengthened or weakened) by the combined effect of biochar properties and soil conditions. Overall, the application of biochars with high ash content (or low carbon content) into sandy soils or acidic soils is highly recommended for increasing plant productivity. This meta-analysis will provide helpful information to elucidate the combined effect of biochar properties and soil conditions on plant growth, which is critical for developing engineered biochar with specific functionality to promote plant production and food security.

Comparison of different crop residue-based technologies for their energy production and air pollutant emission.

Crop residue (CR) based-technology has several benefits, including renewable energy production and improvement in the environment and waste biomass management. However, the quantitative assessment of different CR based-technologies in terms of these benefits is limited. In this study, three typical CR-based technologies, CR biochar (CR-BC), CR direct-fired power (CR-DFP), and CR briquette biofuel (CR-BB), were assessed in terms of energy- and environment-related performances using a mixed-unit input-output life cycle assessment method. The results indicated that CR-BB performed better in energy production and air pollution mitigation than the other two technologies. Energy conversion efficiency was suggested as a key factor in determining the potentials of bioenergy production and environmental improvement. Furthermore, based on the energy demand from the Chinese agricultural sector and CR supply in 2012, the energy supply capacity (ESI) of CR-BC, CR-DFP, and CR-BB was estimated to be 24.3, 2.89, and 53.0, respectively, and their total greenhouse gas (GHG) reduction potential (TRGHG) was estimated to be 1.41 × 106, 4.81 × 106, and 1.68 × 106 t CO2e (CO2 equivalent), respectively. Overall, the CR-DFP and CR-BC are recommended for the high-value utilization of CR resources. The findings of this study could provide a basis for the development of CR-based technologies and CR management policy.

Policy-driven changes in enclosure fisheries of large lakes in the Yangtze Plain: Evidence from satellite imagery.

Enclosure fisheries have accommodated the widespread expansion of aquaculture in many lakes throughout the Yangtze Plain (YP), China, for over four decades. Such practices have increased food provision but have also triggered various detrimental environmental consequences. To restore ecosystem functions, the Chinese government recently implemented specific regulations to remove enclosure fences from lakes throughout the YP. However, little information is available on the spatial and temporal distributions of the enclosure fences, particularly in relation to the enforcement of recent policy changes. Using synthetic aperture radar (SAR) satellite images taken between 2002 and 2018, we conducted the first comprehensive assessment of the interannual changes in enclosure fences in 17 large lakes throughout the YP. Consistent decreases in fence density were found in most lakes after 2015; 15 lakes had >50% of their fences removed, while 9 lakes had >90% removed. The timing and implementation of the development and destruction of enclosure fisheries were related to government policy; before 2015, regional dynamics in enclosure fisheries were attributed to provincial policies, whereas the nearly ubiquitous fence demolition after 2015 was likely a response to national policy. This study represents remotely sensed evidence that demonstrates the importance of both local and national environmental policies and their effectiveness in mitigating ongoing human impacts on vulnerable and valuable natural resources. These findings provide valuable baseline information for future lake environmental monitoring and restoration in the YP region, and the methods used here could be applied to other lacustrine and coastal regions experiencing similar aquaculture activities.