Workplace Objectification Leads to Self-Harm: The Mediating Effect of Depressive Moods.

In the current research, we aimed to extend the literature on workplace objectification and contribute to employees' well-being by exploring whether and how workplace objectification increases self-harm as well as the coping strategy that may weaken the effect. Employing archive data analytic, correlational, longitudinal, and experimental designs, we found that workplace objectification was associated with, or led to, self-harm, irrespective of whether such actions provided an escape from work. This effect could not be simply ascribed to the negative nature of workplace objectification. We further found that depressive moods mediated this effect. The detrimental effect of workplace objectification on self-harm was lessened when employees perceived higher alternatives in life. Theoretical and managerial implications were discussed.

[Molecular markers for predicting the success of micro-TESE in non-obstructive azoospermia].

Non-obstract azoospermia (NOA) is a serious male infertility disease. At present, testicular sperm extraction (micro-TESE) is performed in combination with intracytoplasmic sperm injection (ICSI) technology, NOA patients can have their own consanguine offspring. However, due to the invasiveness and uncertainty of micro-TESE surgery, it is difficult for patients to accept it. Therefore, finding an accurate method to predict the possibility of micro-TESE successful sperm retrival would be beneficial to azoospermia patients. Many genes are transcribed and expressed during spermatogenesis, and molecular assays have irreplaceable sensitivity and specificity in predicting the success sperm retrivel of micro-TESE. This article reviews the methods to predict the success sperm retrivel of micro-TESE including mRNA, non-coding RNA (piRNA, microRNA, cirRNA, tFRNAs) and some protein so far, to provide certain reference value for clinical and subsequent research.

Power Distance Belief and Workplace Communication: The Mediating Role of Fear of Authority.

Power distance is the degree of acceptance of unequal distribution of power in societies. In a high power distance context, the acceptance of inequality conflicts with the operation of modern organizations, which causes obstacles to workplace communication or even triggers workplace accidents due to ineffective communication. We conducted four studies (N = 1063) to explore the relations between and mechanisms of power distance belief and workplace communication. In Study 1, the participants with high power distance belief had ineffective workplace communication-specifically ineffective communication with superiors-but no difference in communication with subordinates and colleagues. We further focused on the mechanism underlying the relationship between power distance belief and communication with superiors. A questionnaire study (Study 2) was conducted in three stages over a three-month period, and an experimental study (Study 3) indicated that fear of authority mediated the negative effect of high power distance on communication with superiors. A cross-culture study (Study 4) re-tested the hypotheses among Chinese and U.S. participants. This research provides insight into the mechanisms that explain the relationship between power distance belief and workplace communication, indicating that fear of authority is significant. Organizations should pay attention to power distance belief and fear of authority, as they may lead to workplace accidents due to communication disasters.

Multi-stage screening cell-free seminal mRNAs to diagnose completion of meiosis and predict testicular sperm retrieval in men with non-obstructive azoospermia.

BACKGROUND: Differential diagnosis of men with subtypes of non-obstructive azoospermia (NOA) is important for their treatment. Many genes are transcripted during meiosis. We hypothesized that some of these genes can be detected in cell-free seminal message RNA (mRNAs) (cfs-mRNA) and be developed as non-invasive biomarkers for diagnosing NOA subtypes. OBJECTIVE: To screen cfs-mRNA to diagnose the completion of meiosis and predict successful sperm retrieval (SR) in men with NOA. MATERIALS AND METHODS: NOA patients who visited our institutes from September 2018 to December 2020 for testicular histopathological diagnosis (n = 109) or testicular SR (n = 92) were screened for participation in the study. Microarray and real-time quantitative polymerase chain reaction were used in five stages to obtain candidate cfs-mRNAs for comparisons between patients with early maturation arrest (eMA, meiosis not completed) and late MA or hypospermatogenesis (meiosis completed) and between NOA patients with successful SR and SR failure. RESULTS: Twelve cfs-mRNAs were selected based on this comparison between men with eMA and hypospermatogenesis and their gene expression and function information. Of these, AKAP1, BOLL, TCP11 and SETX predominantly derived from testes and germ cells were proposed as candidate cfs-mRNAs. Further quantification in men with NOA demonstrated significantly higher levels of BOLL cfs-mRNA (p < 0.0001) in men with late MA or hypospermatogenesis (n = 23), compared with men with eMA (n = 51); and significantly higher levels (p < 0.0001) in patients with successful SR (n = 44) when compared with patients with SR failure (n = 37). Interestingly, with a similar cutoff value, BOLL cfs-mRNA showed high sensitivity and specificity in diagnosing late MA and hypospermatogenesis (>404 copies/ml) and predicting successful SR (>415 copies/ml). Correlation of BOLL mRNA levels was observed in paired semen and testicular tissues. DISCUSSION AND CONCLUSIONS: We propose that BOLL cfs-mRNA is a promising non-invasive marker for diagnosing the completion of meiosis and predicting successful testicular SR in men with NOA.

Social Media and Attitude Change: Information Booming Promote or Resist Persuasion?

Emerging social media platforms such as Twitter and its Chinese equivalent Weibo have become important in information-sharing and communication. They are also gradually becoming stronger in guiding public opinion. When compared with traditional media, these platforms have salient characteristics, such as highly efficient dissemination of information and interactive commentary, which can contribute to information overload. In earlier research, only the effect of social media on attitude change has been studied, but the specific mechanism of this effect in the context of information overload has not been found. To answer this question, we measured the attitude change of participants after they read Weibo posts about street vendors. A 2 (post-attitude: positive posts vs. negative posts) × 4 (reading time: 35 vs. 25 vs. 15 vs. 5 min) experiment was set up, and the Single Category Implicit Attitude Test was used to measure the implicit attitudes. The interaction effect revealed that in both positive and negative posts, less reading time (i.e., information overload) had a stronger influence. Users were more easily persuaded by posts under high overload. Furthermore, the changes in the attitudes of users were not simply stronger with more information. We found three stages, namely, obedience, resistance, and acceptance, with different mechanisms. Therefore, in the positive information overload condition, the attitudes of individuals eventually change in a positive way. In the negative information overload condition, individuals tend to be biased against the group being reported.

A rare karyotype of nonmosaic isodicentric (Y) (p11.31) with azoospermia and short stature.

Chromosome aberration is one of the common causes of male infertility. Isodicentric chromosome is a chromosomal aberration in which two arms of a chromosome are identical in morphology and genetics and connected by two centromeres. We firstly reported a case of infertile male with nonmosaic 46, X, idic (Y) (qter-p11.31::p11.31-qter) but with the sex-determining region Y (SRY). The broken site is the chromosome Y (p11.31). The patients' clinical phenotype was azoospermia and short stature. Fluorescence in situ hybridisation (FISH), chromosomal microarray comparative genomic hybridisation (array CGH) and related molecular analysis were performed. Azoospermia of this case may be caused by the abnormal chromosome structure, which leads to abnormal chromosome synapsis in spermatogenesis. Loss of genes in PAR1 and gain of genes copies in azoospermia factor (AZF) region on the Y chromosome may also contribute to the pathogenesis of azoospermia.

Comprehensive circRNA/miRNA/mRNA analysis reveals circRNAs protect against toxicity induced by BPA in GC-2 cells.

Aim: To identify the circRNAs expression pattern and roles in bisphenol A (BPA) induced germ cell apoptosis. Materials & methods: We performed circRNA/miRNA/mRNA-Seq in 120 µM BPA treated and nontreated GC-2 cells. Bioinformatic analysis, qPCR, apoptosis assays, luciferase report were done in the function analysis. Results: A large number of apoptosis related circRNAs/miRNAs/mRNAs were differentially expressed with competing endogenous RNA network constructed. Interestingly, most investigated upregulated circRNAs, including circDcbld2, circMapk1, circMpp6 and circTbc1d20 showed protective effects in antagonizing BPA toxicity, with the effects individually and synergistically observed. CircMapk1 may take its role by sponging miR-214-3p. Conclusion: circRNAs can play protective roles via sponging miRNAs in toxicity. Some circRNAs may serve as novel targets for BPA toxicity intervention or as biomarkers.