Assessment of visceral and subcutaneous obesity to understand the efficiency of adipose tissue in acute pancreatitis.

BACKGROUND: Fat accumulation in the visceral and subcutaneous regions can trigger fat necrosis during acute pancreatitis (AP). AIMS: We investigated the role of visceral and subcutaneous fat in acute pancreatitis. In this study, we investigated the role of visceral and subcutaneous fat to understand the efficiency of adipose tissue in the AP. MATERIALS AND METHODS: Computed tomography of 68 patients and 68 healthy at the level of L4-5 intervertebral disc were analyzed for body adiposity composition using designated software. Body subcutaneous and visceral composition was measured by using the designated software of the CT. RESULTS: Visceral fat was higher in the control group (198 ± 146) than the group of the AP (155 ± 118) (P = 0.038), whereas the subcutaneous fat was found higher in the AP instead (292 ± 133 to 139 ± 102; P = 0,001). Visceral fat (B = 0,29; P = 0,0013), gender (male) (B = -0.3; P = 0.0122), age (B = 0.274; P = 0.0087), and complication (B = -0.229; P = 0.007) predicted the subcutaneous fat as the dependent variable. In the receiver operating characteristic (ROC), the area under curve was 0.562 (0.402-0.636; 95% CI, P = 0.038) for the visceral fat, while it was 0.906 (0.824-0.962; 95% CI, P < 0.0001) for the subcutaneous fat. Its cutoff was calculated as 183.7 for subcutaneous fat. CONCLUSION: Visceral fat analysis showed a contradiction according to subcutaneous fat that AP was strongly associated with subcutaneous one. The result supports that visceral and subcutaneous fat tissues should have different path of inflammation affecting the AP.

Leukocyte migration inhibition in bacillus Calmette-Guérin vaccinated healthy adults and in tuberculosis assayed by a rapid photoelectric procedure.

A sensitive photoelectric method was used for reading migrations of human peripheral blood leukocytes from agarose microdroplets. Its rapidity enabled the use of a wide range (12 logs) of in vitro concentrations of antigen. Inhibition of migration of leukocytes (LMI) of healthy subjects having had BCG vaccination in childhood was found to occur in two zones of PPD concentrations, one high, from 10(-1) to 1000 micrograms/ml with a peak at 100 micrograms/ml, and one low, from 10(-8) to 10(-2) micrograms/ml. While three-fifths of subjects showed high zone LMI, in one-fifth it was bizonal and in another fifth observed only in the low zone. In patients with active pulmonary tuberculosis LMI, prior to all treatment, was reduced, absent, or replaced by enhanced migration, particularly in the low zone or in both zones. One and a half to four months after treatment LMI was found to be bizonal, enhanced migration having disappeared. These observations suggest the participation of two cell populations with widely different sensitivity to PPD in LMI in tuberculous and BCG vaccinated subjects and the presence of migration stimulatory lymphokine(s) during active tuberculosis.