RESUMO
To assess the temporal dynamics of Middle East respiratory syndrome coronavirus (MERS-CoV) infection in dromedary camels, specimens were collected at 1-2 month intervals from 2 independent groups of animals during April 2013-May 2014 in Al-Ahsa Province, Saudi Arabia, and tested for MERS-CoV RNA by reverse transcription PCR. Of 96 live camels, 28 (29.2%) nasal swab samples were positive; of 91 camel carcasses, 56 (61.5%) lung tissue samples were positive. Positive samples were more commonly found among young animals (<4 years of age) than adults (>4 years of age). The proportions of positive samples varied by month for both groups; detection peaked during November 2013 and January 2014 and declined in March and May 2014. These findings further our understanding of MERS-CoV infection in dromedary camels and may help inform intervention strategies to reduce zoonotic infections.
Assuntos
Camelus/virologia , Infecções por Coronavirus/veterinária , Pulmão/virologia , Coronavírus da Síndrome Respiratória do Oriente Médio , Nariz/virologia , Animais , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Arábia Saudita/epidemiologiaRESUMO
The objective of this study was to report the distinctive anatomical and histological features of the trachea of the Arabian Oryx (Oryx leucoryx). The number of tracheal rings and tracheal length were measured. The diameter, thickness and cross sectional area of tracheal ring were determined at four tracheal regions (cranial cervical (CCR), middle cervical, thoracic inlets and intra thoracic). Tracheal rings were also collected for histological analysis. The mean length of the trachea was 54.1cm +/- 0.73, while the number of tracheal rings varied from 50-51. Tracheal ring measurements (diameter, thickness and cross sectional area) were smallest at the thoracic inlet region. The ratio of the outer transverse to outer vertical and inner transverse to inner vertical diameter ranged between 0.75 and 0.98. The histological analysis revealed that the wall of the trachea consists from four histological layers: a mucosa made of pseudostratified epithelium and lamina propria, submucosa made of dense irregular connective tissue, a hyaline cartilage and a tunica adventitia made of loose connective tissue.
El objetivo fue describir las características anatómicas e histológicas distintivas de la tráquea del Orix de Arabia (Oryx leucoryx). Fueron medidos el número de anillos traqueales y la longitud traqueal. El diámetro, grosor y área de la sección transversal (AST) del anillo traqueal fueron determinados en cuatro regiones (cervical craneal (CCR), medio cervical (MC), entradas torácicas (ET) e intratorácica (EIT)). También se realizó en los anillos traqueales el análisis histológico. La longitud media de la tráquea fue de 54,1+/-0,73 cm, mientras que el número de anillos traqueales varió desde 50 hasta 51. Las mediciones del anillo traqueal (diámetro, grosor y AST) fueron menores en la región de la entrada torácica. La relación de los diámetros transversal exterior a vertical exterior y transversal interno a vertical interno osciló entre 0,75 y 0,98. El análisis histológico reveló que la pared de la tráquea se compone de cuatro capas: mucosa de epitelio pseudoestratificado y lámina propia, submuscosa de tejido conectivo denso irregular, cartílago hialino y túnica adventicia de tejido conectivo laxo.