RESUMO
Quantification of immunoglobulins IgG, IgA, IgM, C3 complement, siderophilin, alpha 2-macroglobulin and haptoglobin in serum by a Behring laser nephelometer coupled with a date processing apparatus was compared with values obtained by the radial immunodiffusion method of Mancini. The precision was not improved by the use of the laser nephelometer as compared to Mancini radial immunodiffusion, but within run precision was better than that among runs for both methods. The analytical recovery studies for laser nephelometry showed a close concordance between the theoretical and measured values for all proteins. The two techniques were also compared by means of correlation studies.
Assuntos
Proteínas Sanguíneas/análise , Complemento C3/análise , Haptoglobinas/análise , Humanos , Imunodifusão/métodos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Lasers , Nefelometria e Turbidimetria/métodos , Transferrina/análise , alfa-Macroglobulinas/análiseRESUMO
Favor, in the Appendix to a paper by Ehling and his collaborators (Ehling et al., 1982), develops a method that tests for the presence of a partially penetrant dominant cataract mutation in a suspected parent (showing lens opacity) outcrossed to a homozygous strain-101 mouse when no individual with lens opacity is observed in the progeny. This method, based on the Chi-square distribution, is examined as to the validity of the Normal approximation. An alternative procedure is discussed.
