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1.
Vet Dermatol ; 34(2): 142-146, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36397673

RESUMO

This case series describes the clinical appearance, histopathological findings and therapeutic trials of proliferative nodular lesions on bilateral ear margins of three domestic cats including two littermates. All therapeutic trials were unsuccessful. While the aetiology remains unclear, this report highlights different hypotheses in presenting this unusual inflammatory and fibroblastic dermatosis in cats.


Assuntos
Doenças do Gato , Dermatopatias , Animais , Gatos , Mastócitos , Orelha , Diagnóstico Diferencial , Dermatopatias/veterinária
2.
PLoS One ; 10(4): e0121274, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25874801

RESUMO

An old world fruit bat Pteropus giganteus, held in captivity and suffering from necrosis of its wing digits, failed to respond to antibiotic therapy and succumbed to the infection. Samples submitted to the National Centre for Foreign Animal Disease were tested for viral infection. Vero E6 cells exhibited minor but unique cytopathic effects on second blind passage, and full CPE by passage four. Utilizing an unbiased random amplification technique from cell culture supernatant, we identified a bacterium belonging to the Bradyrhizobiaceae. Purification of cell culture supernatant on TY media revealed a slow growing bacterial isolate. In this study using electron microscopy, 16S rRNA gene analysis and whole genome sequencing, we identify a novel bacterial species associated with the site of infection belonging to the genus Afipia. This genus of bacteria is very diverse, with only a limited number of species characterized. Afipia felis, previously described as the etiological agent to cause cat scratch disease, and Afipia septicemium, most recently shown to cause disease in humans, highlight the potential for members of this genus to form a branch of opportunistic pathogens within the Bradyrhizobiaceae. Increased utilization of next generation sequencing and genomics will aid in classifying additional members of this intriguing bacterial genera.


Assuntos
Bradyrhizobiaceae/isolamento & purificação , Quirópteros/microbiologia , Necrose/microbiologia , Asas de Animais/microbiologia , Afipia/patogenicidade , Animais , Bradyrhizobiaceae/genética , Bradyrhizobiaceae/patogenicidade , Doença da Arranhadura de Gato/genética , Doença da Arranhadura de Gato/microbiologia , Gatos , Genoma Bacteriano , Humanos , Necrose/fisiopatologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Asas de Animais/fisiopatologia
3.
Zool Stud ; 54: e21, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-31966108

RESUMO

BACKGROUND: The biologic attributes of the endocrine pancreas and the comparative endocrinology of islet amyloid polypeptide (IAPP) of fish are not well described in the literature. This study describes the endocrine pancreasof one teleostean fish. Ten captive Atlantic wolffish (Anarhichas lupus)from the Montreal Biodome were submitted for necropsy and their pancreata were collected. RESULTS: Grossly, all the fish pancreata examined contained 1-3 nodules of variable diameter (1-8 mm). Microscopically, the nodules were uniform, highly cellular, and composed of polygonal to elongated cells. Immunofluorescence for pancreatic hormones was performed. The nodules were immunoreactive for insulin most prominent centrally, but with IAPP and glucagon only in the periphery of the nodules. Exocrine pancreas was positive for chromogranin A. Not previously recognized in fish, IAPP immunoreactivity occurred in α, glucagon-containing, cells and did not co-localize with insulin in ß cells. The islet tissues were devoid of amyloid deposits. IAPP DNA sequencing was performed to compare the sequence among teleost fish and the potency to form amyloid fibrils. In silico analysis of the amino acid sequences 19-34 revealed that it was not amyloidogenic. CONCLUSIONS: Amyloidosis of pancreatic islets would not be expected as a spontaneous disease in the Atlantic wolffish. Our study underlines that this teleost fish is a potential candidate for pancreatic xenograft research.

4.
Avian Pathol ; 41(4): 345-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22834547

RESUMO

Microfilariae are considered non-pathogenic in wild birds. The objective of the current communication is to report host reactions to microfilarial infection of unusual intensity in emaciated boreal owls (Aegolius funereus). An unusually large number of boreal owls (n = 21) were submitted to the Canadian Cooperative Wildlife Health Center-Quebec Region for post-mortem examination during the winter of 2009. Nineteen out of 21 birds were considered emaciated based on atrophy of adipose tissue and pectoral muscles and suboptimal weight. A microscopic examination of a subset of nine owls revealed the presence of microfilariae in six owls. Three of the birds with a heavy parasite burden had masses of larval nematodes obstructing large vessels of the lungs. The emaciated owls are believed to have died from starvation due to a cyclic decrease in prey abundance in the boreal forest. This cycle also drives winter movements of boreal owls to urbanized areas of southern Quebec, presumably accounting for the large number of birds submitted in 2009. In the most severely infected owls, the extreme microfilarial burden might have caused an alteration in circulatory dynamics, gaseous exchanges and also probably some metabolic cost. Consequently, microfilariae could have significantly contributed to the death of some of these owls.


Assuntos
Doenças das Aves/epidemiologia , Emaciação/veterinária , Microfilárias/isolamento & purificação , Infecções por Nematoides/veterinária , Estrigiformes/parasitologia , Animais , Animais Selvagens , Doenças das Aves/mortalidade , Doenças das Aves/parasitologia , Vasos Sanguíneos/parasitologia , Emaciação/epidemiologia , Emaciação/parasitologia , Feminino , Pulmão/parasitologia , Masculino , Microfilárias/citologia , Infecções por Nematoides/epidemiologia , Infecções por Nematoides/parasitologia , Carga Parasitária/veterinária , Parasitemia/veterinária , Quebeque/epidemiologia , Estações do Ano , Estresse Fisiológico/fisiologia
6.
Can Vet J ; 50(12): 1251-6, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20190973

RESUMO

Information related to infection of wild rodents or lagomorphs in Canada by Francisella tularensis, Yersinia pestis, other Yersinia spp., and Clostridium piliforme was searched for this study. Reports on tularemia in humans linked to these species came from diagnostic databases, literature, wildlife health specialists, and public health agencies. Tularemia has been diagnosed in 8 species of wild rodent and 2 species in the genus Lepus in Canada. Tularemia occurred in wild animals, or in humans associated with these species, in all jurisdictions except the Yukon and Nunavut. Tularemia was diagnosed most frequently in beaver, muskrats, and snowshoe hares, and although tularemia is closely linked to cottontail rabbits in the USA, it has not been reported in cottontails in Canada. Tularemia in humans was associated with muskrats and hares more commonly than with beaver. Plague was diagnosed in bushy-tailed woodrats in British Columbia in 1988. Based on surveys, Y. pestis may occur enzootically in southern Alberta, Saskatchewan, and British Columbia. Infection with Yersinia pseudotuberculosis and Y. enterocolitica has been diagnosed in beaver, muskrats, and snowshoe hares in many provinces. Tyzzer's disease has been diagnosed in muskrats in British Columbia, Saskatchewan, Ontario, and Quebec and in snowshoe hares in Ontario. Infection with these bacteria is likely much more frequent than indicated by diagnostic records.


Assuntos
Infecções por Clostridium/veterinária , Peste/veterinária , Doenças dos Roedores/epidemiologia , Tularemia/veterinária , Yersiniose/veterinária , Animais , Animais Selvagens , Canadá/epidemiologia , Infecções por Clostridium/epidemiologia , Infecções por Clostridium/transmissão , Reservatórios de Doenças/veterinária , Lebres , Humanos , Lagomorpha , Peste/epidemiologia , Peste/transmissão , Doenças dos Roedores/transmissão , Roedores , Tularemia/epidemiologia , Tularemia/transmissão , Yersiniose/epidemiologia , Yersiniose/transmissão , Zoonoses
7.
J Vet Diagn Invest ; 16(4): 326-9, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15305745

RESUMO

A case of West Nile virus (WNV) infection in a captive 4-month-old Arctic wolf (Canis lupus) is described. The animal had vomiting, anorexia, and ataxia before death. Histopathology revealed multifocal severe renal lymphoplasmacytic vasculitis, mostly affecting small arterioles, with fibrinoid degeneration of some vessel walls. Many small foci of gliosis were detected in the cerebral cortex. West Nile virus was demonstrated in the kidneys and cerebrum by immunohistochemistry and polymerase chain reaction. The described renal changes represent a novel pathological finding of WNV infection.


Assuntos
Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/patogenicidade , Lobos/virologia , Doenças dos Animais/virologia , Animais , Animais Domésticos , Córtex Cerebral/virologia , Imuno-Histoquímica/veterinária , Rim/virologia
8.
Aquat Toxicol ; 69(1): 35-49, 2004 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-15210296

RESUMO

Cytochromes P450 (CYP, phase I) and conjugating (phase II) enzymes can be induced by and influence the toxicokinetics (metabolism) and toxicity of xenobiotic contaminants in exposed organisms. Beluga whale (Delphinapterus leucas) from the endangered St. Lawrence (SL) River Estuary population exhibit deleterious health effects and various severe pathologies that have been associated with contaminant exposure. In contrast, such effects (e.g. reproductive and immunological impairment) are generally less frequent in less exposed populations in the Canadian Arctic (CA). In the present study, opportunistic sampling resulted in the collection immediately after death of liver tissue from a single female neonate SL beluga (SL6) and male and female CA beluga (n=10) from the Arviat region of western Hudson Bay, in addition to sampling of stranded carcasses of male and female SL beluga (n=5) at least 12 h postmortem. We immunologically characterized cross-reactive proteins of hepatic microsomal CYP1A, CYP2B, CYP3A, CYP2E, epoxide hydrolase (EH) and uridine diphosphoglucuronosyl transferase (UDPGT) isozymes. Cross-reactive proteins were found in all SL and CA beluga using anti-rat CYP1A1, anti-rainbow trout CYP3A, anti-human CYP2E1, anti-rabbit EH and anti-human UDPGT1A1 polyclonal antibodies (Abs), whereas faintly cross-reactive CYP2B proteins were only found in SL6 and the CA samples using an anti-rabbit CYP2B1 Ab. In corresponding catalytic activity assessments, only SL6 and all CA beluga microsomal samples exhibited CYP1A-mediated 7-ethoxyresorufin O-deethylase (EROD) activity (51-260 pmol/mg/min), CYP3A-mediated activity (113-899 pmol/mg/min) based on the formation of 6beta-hydroxytestosterone using a testosterone hydroxylase assay, and UDPGT activity (830-4956 pmol/mg/min) based on 1-naphthylglucuronide formation. The marginal cross-reactivity with the anti-CYP2B1 Ab and lack of catalytically measurable hydroxytestosterone isomers associated with CYP2B-type activity in all the SL and CA animals is suggestive of low CYP2B-type enzyme expression in beluga. The absence of measurable total P450 enzyme levels and catalytic activities in samples from the stranded SL belugas suggested catalytically inactive enzymes as a consequence of tissue degradation related due to the time delay of sample collection after death. However, all SL and CA animals demonstrated similar, immunologically cross-reactive phase I and II hepatic enzyme profiles, which is suggestive of the importance of metabolism in the toxicokinetics and fate of xenobiotics in animals from both populations


Assuntos
Anticorpos/imunologia , Citocromo P-450 CYP1A1/metabolismo , Epóxido Hidrolases/metabolismo , Glucuronosiltransferase/metabolismo , Baleias/imunologia , Baleias/metabolismo , Animais , Regiões Árticas , Canadá , Cromatografia Líquida de Alta Pressão , Reações Cruzadas , Citocromo P-450 CYP1A1/imunologia , Epóxido Hidrolases/imunologia , Glucuronosiltransferase/imunologia , Hidroxitestosteronas/metabolismo , Isoenzimas/imunologia , Isoenzimas/metabolismo , Microssomos Hepáticos/enzimologia , Água do Mar , Espectrofotometria
9.
Environ Toxicol Chem ; 22(7): 1497-506, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12836974

RESUMO

Sixty-four adult captive American kestrels (Falco sparverius) were exposed to a dietary source of halogenated dimethyl bipyrroles (HDBPs), a group of organohalogens proposed to have a natural origin. Adult kestrels were assigned to four treatment groups that were administered a different dose of HDBPs [high, 140 microg/(bird x d); medium, 4.3 microg/(bird x d); low, 0.13 microg/(bird x d); control, 0 microg/(bird x d)]. Exposure began prior to pairing and continued during breeding until approximately 28 d after hatching of the final eggs. Juvenile and adult kestrels accumulated HDBPs in carcass tissue, plasma, and liver. In addition, HDBPs were transferred to yolks during egg development. Low dietary absorption efficiencies (0.011-0.029) indicated that HDBPs were effectively cleared from kestrels either from increased elimination, metabolism, or a combination of the two processes. Reproductive and morphological endpoints examined for HDBP-induced changes included egg volume, egg mass, eggshell thickness, egg fertility, clutch size, liver and whole body mass, antebrachium, central retrice, tarsus, ninth primary, and wing cord lengths. Few statistically significant dose-dependent effects were observed. Hepatic tissue samples from selected individuals underwent histomorphological evaluation. Although the hepatic function was not evaluated specifically in these birds, no clinical signs suggestive of liver disease or gross hepatic anomalies were observed. Only minor histomorphological changes were detected in the hepatic tissue. The lack of serious effects suggests that HDBPs are not an acute reproductive threat to avian populations.


Assuntos
Poluentes Ambientais/farmacocinética , Poluentes Ambientais/toxicidade , Pirróis/farmacocinética , Pirróis/toxicidade , Aves Predatórias/fisiologia , Reprodução/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Embrião não Mamífero/efeitos dos fármacos , Desenvolvimento Embrionário , Feminino , Halogênios , Fígado/química , Masculino , Óvulo/efeitos dos fármacos , Óvulo/patologia , Aves Predatórias/anatomia & histologia , Aves Predatórias/embriologia , Distribuição Tecidual
11.
Environ Health Perspect ; 110(3): 285-92, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11882480

RESUMO

A population of approximately 650 beluga (Delphinapterus leucas) inhabits a short segment of the St. Lawrence estuary (SLE). Over 17 years (1983-1999), we have examined 129 (or 49%) of 263 SLE beluga carcasses reported stranded. The major primary causes of death were respiratory and gastrointestinal infections with metazoan parasites (22%), cancer (18%), and bacterial, viral, and protozoan infections (17%). We observed cancer in 27% of examined adult animals found dead, a percentage similar to that found in humans. The estimated annual rate (AR) of all cancer types (163/100,000 animals) is much higher than that reported for any other population of cetacean and is similar to that of humans and to that of hospitalized cats and cattle. The AR of cancer of the proximal intestine, a minimum figure of 63 per 100,000 animals, is much higher than that observed in domestic animals and humans, except in sheep in certain parts of the world, where environmental contaminants are believed to be involved in the etiology of this condition. SLE beluga and their environment are contaminated by polycyclic aromatic hydrocarbons (PAHs) produced by the local aluminum smelters. The human population living in proximity of the SLE beluga habitat is affected by rates of cancer higher than those found in people in the rest of Québec and Canada, and some of these cancers have been epidemiologically related to PAHs. Considered with the above observations, the exposure of SLE beluga to PAHs and their contamination by these compounds are consistent with the hypothesis that PAHs are involved in the etiology of cancer in these animals.


Assuntos
Exposição Ambiental , Poluentes Ambientais/efeitos adversos , Neoplasias/etiologia , Neoplasias/veterinária , Hidrocarbonetos Policíclicos Aromáticos/efeitos adversos , Baleias , Animais , Causas de Morte , Estudos Epidemiológicos , Feminino , Incidência , Masculino , Neoplasias/epidemiologia , Quebeque
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