[Cytomegalovirus infection in Omsk region].

Epidemiologic studies of prevalence of cytomegalovirus infection (CMVI) in Omsk region during a period of 15 years showed 1.6-fold increase of population seropositivity to CMV with rate of increase +2.94. In recent years antibodies to CMV were detected in 90.3 - 94.8% of cases. Diagnostics of innate and acquired CMVI was improved. Feasibility of detection of infectious process activity during complex use of new laboratory methods (IgA detection, assessment of IgG avidity, IgG to individual antigens in immunoblotting as well as detection of DNA and "early phase" viral ptoteins) was demonstrated. High rates of infection in population determine the necessity of revision of strategy for examination of children and adults, including screening of pregnant woman, on this infection.

[Epidemiological aspects of cytomegalovirus infection in babies aged under one]. / Epidemiologicheskie aspekty tsitomegalovirusnoi infektsii u detei pervogo goda zhizni.

Sreening data obtained on babies aged under one and selected by random (1,910 children) or target (2,658 children) choice for cytomegalovirus (CMV) infection during the period of 10 years (1992-2001) were compared with mortality rate. The methods used were enzyme immunoassay, immunofluorescence and polymerase chain reaction. The babies were divided as follows: newborn infants (group I), babies aged 1-3 months (group II), 4-6 months (group III) and 7-12 months (group IV). Specific clinical features of CMV infection in newborn infants were studied on 69 cases (37--with CMV monoinfection and 32--with mixed infection). The serological screening revealed a 2.1-fold growth of the infection rate among randomly selected newborn infants during the 10 year period. Positive correlation between the infection rate among children of this age group and the neonatal mortality rate was established. High risk factors of CMV infection were revealed as well as increased infection rate and frequency of clinical cases with the prevailing neurological pathology in group III. Early diagnosis, the exclusion of mixed infections and early adequate therapy were shown to play a decisive role in the outcome of the disease. The algorithm of epidemiological surveillance and the regional program of prophylaxis were worked out.

[The epidemiological surveillance of hospital infections linked to methicillin-resistant Staphylococcus aureus]. / Epidemiologicheskii nadzor za vnutribol'nichnymi infektsiiami, sviazannymi s metitsillinrezistentnymi Staphylococcus aureus.

As revealed in the realization of the epidemiological surveillance of hospital infections caused by methicillin-resistant S. aureus (MRSA) in different types of hospital, MRSA strains causing purulent inflammatory diseases belong to different clones. The complex marking of MRSA made it possible to determine the presence of the same clone in different hospitals and to detect the outbreaks of hospital infections caused by different clones of MRSA in one hospital. It was found necessary to supplement the commercial international phage-typing set with pages permitting the detection of the specific system of restriction-modification in MRSA.

[Experimental substantiation of population heterogeneity in methicillin resistant Staphylococcus aureus]. / Eksperimental'noe obosnovanie geterogennosti populiatsii metitsillinrezistentnykh Staphylococcus aureus.

A comprehensive intraspecies typing of the cultures of MRSA collected during inspection of drug resistance in causative agents of intrahospital infections was performed. The following parameters were investigated: antibiotic resistance, toxin production, sensitivity to the phages of the International Set and the phages of an experimental collection providing the isolation of strains with definite specificity of the restriction-modification system. Different clones of methicillin resistant S. aureus were found to be circulating on the territory of the CIS.

[Serological surveillance of diphtheria]. / Serologicheskii nadzor za difteriei.

The serological method (indirect hemagglutination test) has been used in the surveillance of diphtheria infection. The structure of immunity to diphtheria in the population of the region has been studied. The possibility of increasing intervals between booster immunizations has been confirmed. The introduction of planned booster immunizations of the adult population with diphtheria toxoid is proposed.