Diagnostic criteria in Sézary's syndrome: a multiparameter study of peripheral blood lymphocytes in 32 patients with erythroderma.

In order to define additional diagnostic criteria for the early diagnosis of Sézary's syndrome (SS), peripheral blood lymphocytes of 32 patients with erythroderma, including 8 patients with SS, 4 patients with erythrodermic mycosis fungoides, 14 patients with an erythroderma on the basis of atopic or chronic dermatitis, and 6 patients with erythrodermic psoriasis, were investigated by computer-assisted morphometry. The degree of nuclear indentation, expressed as the nuclear contour index (NCI), was measured on electron micrographs. The mean NCI and the percentages of cerebriform mononuclear cells (CMC), defined by a NCI greater than or equal to 6.5, were calculated. In addition, the percentages of lymphocytes, T and B cells, and the distribution of T-cell sub-populations as defined by Fc-receptors (T mu, T gamma) and monoclonal antibodies (OKT3, OKT4, OKT8, HLA-DR) were determined. Statistical analysis showed as most discriminating parameters for the differentiation between SS and benign forms of erythroderma: high percentages of lymphocytes (50% or more), an expanded OKT3+, OKT4+ population with an OKT4/OKT8 ratio greater than 10, a mean NCI value greater than or equal to 5.5, the presence of more than 20% CMC, as well as the presence of cells with a NCI greater than or equal to 11.5. The total leukocyte and lymphocyte counts, as well as the percentages of B, T, T mu, and T gamma cells had limited value for the early diagnosis of SS.

Autoantibodies against Tmu and B lymphocytes in patients with rheumatoid arthritis.

Patients with rheumatoid arthritis have decreased numbers of T mu lymphocytes in their peripheral blood. To find out whether these low number of T mu lymphocytes were associated with the presence of anti-lymphocyte antibodies, the sera of 27 patients with definite or classical rheumatoid arthritis (RA) were investigated for the presence of autoantibodies against subsets of lymphocytes. In addition the numbers of T, T mu, T gamma and B lymphocytes in the peripheral blood of these patients were investigated. Patients with active RA showed lower numbers of T mu lymphocytes in their peripheral blood than patients with inactive RA. However, both groups of RA patients had significantly decreased numbers of T mu lymphocytes in their peripheral blood as compared with 22 age matched healthy donors. Moreover, mainly in patients with active RA cold reactive antibodies were found directed against T mu and B lymphocytes, but never against T gamma lymphocytes of healthy donors. Similar results were found in the indirect immunofluorescence procedure when tested for reactivity against T-cell subsets. This serum reactivity was not caused by rheumatoid factors or antinuclear antibodies. Since RA sera after precipitation with 2.5% polyethyleneglycol, still showed cytotoxicity against T and B lymphocytes, it is suggested that this serum reactivity is not caused by immune complexes but by antibodies.