[Solid-phase immunoenzyme method of quantitative determination of barbiturates]. / Tverdofaznyi immunofermentnyi method kolichestvennogo opredeleniia barbituratov.

A solid-phase competitive enzyme immunoassay for measurements of barbiturates in biologic tissues of human body was developed. Antibodies to barbiturate derivatives, obtained by rabbit immunization with conjugated antigen (barbamyl conjugate with ovalbumin) were used as reagents in the assay. Together with the chromatographic procedures the developed EIA may be used in practical forensic medicine and narcology.

[Chemical synthesis and cloning of the human epidermal growth factor gene]. / Khimicheskii sintez i klonirovanie gena épidermal'nogo faktora rosta cheloveka.

The solid-phase phosphotriester method was used to synthesise 24 oligodeoxyribonucleotides, which were enzymatically joined together to give the human epidermal growth factor gene and its analogue containing Leu codon in position 21. The primary structure of the cloned genes were confirmed by the Maxam-Gilbert technique. It is demonstrated that the high purity degree of oligonucleotides allows to synthesise and clone genes without purification of intermediate fragments.