Correlations begin at home: drivers of co-occurrence patterns in personality and cognitive ability in wild populations of zebrafish.

Aquatic habitats are extremely dynamic, with constantly changing ecological factors, which has now been exacerbated due to human-induced rapid environmental change. In such variable environments, it becomes essential to understand how personality and cognition in organisms affect the adaptability of individuals to different habitat conditions. To test this, we studied how personality-related traits as well as cognitive ability differ between populations of wild-caught zebrafish (Danio rerio) from habitats that differed in various environmental factors. We measured emergence into a novel environment as an indicator of boldness, and performance in a spatial task inferred from feeding latencies in a maze over repeated trials to assess learning and memory, as an indicator of cognitive ability. We found that personality affects cognition and although bolder fish are better learners, they show poorer retention of memory across populations. Although personality and cognitive ability varied between habitats, the patterns of their correlations remained similar within each population. However, the individual traits (such as sex and size) that were drivers of personality and cognition differed between the habitats, suggesting that not only do behavioral traits vary between populations, but also the factors that are important in determining them. Personality and cognitive ability and the correlations between these traits determine how well an organism performs in its habitat, as well as how likely it is to find new habitats and adapt to them. Studying these across wild zebrafish populations helps predict performance efficiencies among individuals and also explains how fish adapt to extremely dynamic environments that can lead to variation in behavioral traits and correlations between them. This study not only sheds light on the drivers of interindividual variation and co-occurrence patterns of personality and cognition, but also individual and population factors that might have an effect on them.

Sex and Population Drive Interindividual Variations in a Cognitive Task Across Three Populations of Wild Zebrafish.

Animal personality refers to the consistency of variation in behavior among individuals which may be the driving force behind variations in complex behaviors as well. Individual personality could predict how well an organism would perform in behavior and cognition related tasks, as well as survive and thrive in its environment. Therefore, we would expect inter-individual variations in many behaviors, which would persist even if habituation to the experimental setup occurs, which generally results in convergence of behavior (i.e., the difference between individuals becomes less pronounced). Our study used wild-caught zebrafish (Danio rerio) from three natural habitats with differing ecological regimes, to understand how consistency and repeatability in specific traits such as boldness, exploration, and spatial ability varies across and within populations even when habituation causes change in behavior. We found that the extent of individual variation differs between populations, with dynamic habitats showing similar repeatability. This indicates that habitat conditions are important drivers of individual variation in addition to other factors, such as sex or size of individuals within populations. Although we found that sex and size played an important role within some populations for some behaviors, in others, the variation was likely caused by other factors (for example, ecological factors such as vegetation and/or resource availability), for which we have not accounted. This study underlines the importance of studying inter-individual differences as the phenomenon that underpins multiple behavioral traits and explains the possible role of environmental and inherent factors that drive these differences.

Wild Zebrafish Sentinels: Biological Monitoring of Site Differences Using Behavior and Morphology.

Environmental change poses a devastating risk to human and environmental health. Rapid assessment of water conditions is necessary for monitoring, evaluating, and addressing this global health danger. Sentinels or biological monitors can be deployed in the field using minimal resources to detect water quality changes in real time, quickly and cheaply. Zebrafish (Danio rerio) are ideal sentinels for detecting environmental changes due to their biomedical tool kit, widespread geographic distribution, and well-characterized phenotypic responses to environmental disturbances. Here, we demonstrate the utility of zebrafish sentinels by characterizing phenotypic differences in wild zebrafish between two field sites in India. Site 1 was a rural environment with flowing water, low-hypoxic conditions, minimal human-made debris, and high iron and lead concentrations. Site 2 was an urban environment with still water, hypoxic conditions, plastic pollution, and high arsenic, iron, and chromium concentrations. We found that zebrafish from Site 2 were smaller, more cohesive, and less active than Site 1 fish. We also found sexually dimorphic body shapes within the Site 2, but not the Site 1, population. Advancing zebrafish sentinel research and development will enable rapid detection, evaluation, and response to emerging global health threats.

Bolder and Brighter? Exploring Correlations Between Personality and Cognitive Abilities Among Individuals Within a Population of Wild Zebrafish, Danio rerio.

Within populations, individual differences in behavioral and cognitive traits are dependent on the habitat and specific contexts, such as the presence of a predator or other risks. The ability to show variable responses to changing conditions can be of immense survival advantage to organisms. We studied individual differences in specific personality traits, such as boldness, exploration, and spatial ability, and the effect of these traits on learning ability and memory in the presence of a predatory threat, among wild caught zebrafish (Danio rerio). Under laboratory conditions, individuals were trained to perform a simple navigation task, and their performance, exploration, boldness traits were measured, along with learning and memory abilities under two contexts (i.e., in the presence and absence of a predator). Our results revealed that fish showed a clear decline in emergence time, exploration time, and feeding latency over trials, indicative of learning, and further tests for memory also showed memory retention. While the presence of a predator increased emergence time and latencies for navigating, indicating declines in boldness and exploration, these were found to be correlated to different personalities among the individuals and dependent on their sex. While females tended to be bolder and learned the spatial task faster, they showed lower memory retention abilities than males. Personality traits were also found to affect cognitive abilities among individuals. In general, the presence of a predator decreased performance latencies. However, bolder individuals were less affected and emerged more quickly from the refuge chamber than shy individuals. Our results point to the complex interplay of ecological context along with inherent correlations across personality traits that decide the overall personality and cognitive responses among individuals even within populations. These findings thus highlight the importance of an inclusive approach that combines personality and cognition studies for understanding variations within populations.

Collective Behavior in Wild Zebrafish.

Anthropogenic change is expected to alter environments at alarming rates. To predict the impact of modified environments on social behavior, we must study the relationship between environmental features and collective behavior in a genetically tractable model, zebrafish (Danio rerio). Here, we conducted a field study to examine the relationship between salient environmental features and collective behavior in four populations of zebrafish. We found zebrafish in flowing water formed volatile groups, whereas those in still water had more consistent membership and leadership. Groups in fast-flowing water were large (up to 2000 fish) and tightly knit with short nearest neighbor distances, whereas group sizes were smaller (11 fish/group) with more space between individual fish in still and slow-flowing water. These observations point to a possible profound role of water flow in influencing collective behavior in wild zebrafish.

Temporal patterns and sex differences in dyadic interactions in a wild zebrafish population.

Male-female interactions in several group living organisms, including some fish species tend to be dynamic and play a key role in determining their mating and courtship behavior. Laboratory-bred zebrafish (Danio rerio) strains are one of the most widely used model systems in various fields of biology. While research on wild zebrafish behavior is gaining ground, our knowledge about their mating ecology and mating strategies is still limited. We investigated diel temporal patterns in inter-sex dyadic interactions among wild zebrafish and the occurrence of behavioral dimorphism in their interactive behaviors. We observed randomly paired male and female individuals at three distinct time intervals (early morning, morning and afternoon sessions) in the day and collected occurrence data for six discrete inter-individual interactive behaviors that were associated with mating, aggression, and display. We used generalized linear mixed models to examine the effect of time, sex of the individual and presence of oviposition substrate on these behavioral traits. We found a higher incidence of mating-associated behavior during the early parts of the day which declined by the afternoon. These mating-associated behaviors were also dependent on the presence of gravel substrate for egg-laying compared to other behaviors. This work is the first of its kind that details patterns in behavioral dimorphism between sexes in zebrafish. Our results throw light on the complex dynamics of male-female interactions in a group living externally fertilizing species and can have implications in designing experiments involving behavioral testing of zebrafish which is increasingly being done in toxicological studies and laboratory breeding.

Sleep deprivation negatively impacts reproductive output in Drosophila melanogaster.

Most animals sleep or exhibit a sleep-like state, yet the adaptive significance of this phenomenon remains unclear. Although reproductive deficits are associated with lifestyle-induced sleep deficiencies, how sleep loss affects reproductive physiology is poorly understood, even in model organisms. We aimed to bridge this mechanistic gap by impairing sleep in female fruit flies and testing its effect on egg output. We found that sleep deprivation by feeding caffeine or by mechanical perturbation resulted in decreased egg output. Transient activation of wake-promoting dopaminergic neurons decreased egg output in addition to sleep levels, thus demonstrating a direct negative impact of sleep deficit on reproductive output. Similarly, loss-of-function mutation in dopamine transporter fumin (fmn) led to both significant sleep loss and lowered fecundity. This demonstration of a direct relationship between sleep and reproductive fitness indicates a strong driving force for the evolution of sleep.