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ObjectiveTo investigate the performance of serological test and dynamics of serum antibody with the progress of SARS-CoV-2 infections. MethodsA total of 419 patients were enrolled including 19 confirmed cases and 400 patients from fever clinics. Their serial serum samples collected during the hospitalization were menstruated for IgM and IgG against SARS-CoV-2 using gold immunochromatographic assay and chemiluminescence immunoassay. We investigated whether thermal inactivation could affect the results of antibody detection. The dynamics of antibodies with the disease progress and false positive factors for antibody testing were also analyzed. ResultsThe positive rate of IgG detection was 91.67% and 83.33% using two CLIA, respectively. However, the IgM positive rate was dramatically declined might due to the lack of blood samples at early stages of the disease. The chemiluminescence immunoassay had a favorable but narrow linear range. Our work showed increased IgG values in serums from virus-negative patients and four negative samples were IgG weak-positive after thermal incubation. Our data showed the specificity of viral N+S proteins was higher than single antigen. Unlike generally thought that IgM appeared earlier than IgG, there is no certain chronological order of IgM and IgG seroconversion in COVID-19 patients. It was difficult to detect antibodies in asymptomatic patients suggesting that their low viral loads were not enough to cause immune response. Analysis of common interferent in three IgG false-positive patients, such as rheumatoid factor, proved that false positives were not caused by these interfering substances and antigenic cross-reaction. ConclusionsViral serological test is an effective means for SARS-CoV-2 infect detection using both chemiluminescence immunoassay and gold immunochromatographic assay. Chemiluminescence immunoassay against multi-antigens has obvious advantages but still need improve in reducing false positives.
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Objective To observe the clinical effects of puerarin injection combined with reduced glutathione in the treatment of stage Ⅲ of diabetic nephropathy.Methods One hundred inpatients with stage Ⅲ of diabetic nephropathy in the Chongqing Municipal Hospital of Traditional Chinese Medicine from January 2015 to October 2016 were selected and divided into the observation group and control group according to the random number table method,50 cases in each group.Both groups took the basic therapy of controlling blood sugar,blood lipids and blood pressure.On the basis of general treatment,the control group was added with reduced glutathione injection 1.8 g/d,while on the basis of the control treatment,the observation group was added with puerarin injection 300 mg/d.Both groups were treated for 4 weeks.Fasting plasma glucose (FPG),2 h postprandial plasma glucose (2 h PG),blood pressure,blood fat,serum creatinine(SCr),blood urea nitrogen (BUN),24 h urinary total protein,urine albumin,urine β2 micro-globulin (β2-MG),NAG,and other indicators before and after treatment were detected in the two groups.Results FPG,2 h PG,blood pressure and blood fat after treatment in the observation group and control group were improved,but the improvement in the observation group was better than that in the control group (P＜0.05);the levels of SCr,BUN,24 h urine protein,urine albumin and β2 micro-globulin and urine NAG in the observation group and control group patients were reduced to some extent(P＜ 0.05),which were significantly improved compared with before treatment (P＜0.05),but the improvement degree in the observation group were better than that in the control group (P＜0.05).Conclusion Puerarin injection combined with reduced glutathione injection has obvious therapeutic effect in treating the stageⅢ of diabetic nephropathy,and has less adverse reactions,is safe and effective and can be promoted and applied in clinic.
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OBJECTIVE:To observe the clinical efficacy and ADR of ultrasound debridement combined with Huangma tinc-ture in the treatment of diabetic foot ulcer. METHODS:90 diabetic foot ulcer patients were randomly divided into combination treatment group(ultrasound debridement combined with Huangma tincture wet compress),Huangma tincture group(Huangma tinc-ture wet compress) and control group (vaseline) with 30 cases in each group. 3 months after treatment,Ulcer healing rate,cure time,effective time,recovery time,cure rate and ADR were observed in each group. RESULTS:The ulcer healing rate of combi-nation treatment group was significantly higher than that of Huangma tincture group and control group,with statistical significance (P<0.05);there was statistical significance between Huangma tincture group and control group(P<0.05). Mean cure time,effec-tive time and recovery time of combination treatment group were all significantly lower than Huangma tincture group and control group,with statistical significance(P<0.05);there was statistical significance between Huangma tincture group and control group (P<0.05). Cure rate of combination treatment group was higher than that of Huangma tincture group and control group,with statis-tical significance (P<0.05);there was statistical significance between Huangma tincture group and control group (P<0.05). No ADR was found in combination treatment group and Huangma tincture group. CONCLUSIONS:The ultrasound debridement com-bined with Huangma tincture in the treatment of diabetic foot ulcer improve healing rate and shorten healing time significantly with-out obvious ADR. It has good clinical efficacy.
