RESUMO
RESUMO O conhecimento das populações bacterianas responsáveis pela biodegradação dos resíduos sólidos dispostos em aterros sanitários pode levar ao desenvolvimento de alternativas tecnológicas viáveis para o tratamento e a estabilização dos resíduos, resultando em impactos positivos para a operação de aterros, a recuperação de energia, a saúde pública e o meio ambiente. O objetivo deste trabalho foi caracterizar a diversidade bacteriana associada à biodegradação de resíduos sólidos urbanos (RSU) aterrados em uma célula experimental no município de Campina Grande, Paraíba. O estudo abrangeu as etapas de construção, planejamento estatístico dos bairros de Campina Grande para coleta e preenchimento da célula experimental com RSU. As amostras de DNA das bactérias encontradas nos RSU foram extraídas com o Kit Power Soil DNA Isolation. Em seguida, foi realizada a análise genética com primers universais para bactérias via reação em cadeia da polimerase (PCR) e eletroforese em gel de gradiente com desnaturante (DGGE), e, por fim, sequenciamento genético (região 16S do RNAr). Após o exame microbiológico, as principais bactérias associadas aos táxons foram: Uncultured Pseudomonas sp, Uncultured bacterium, Enterobacter sp., Klebsiella pneumoniae e Uncultured Bacillus sp., sugerindo que nos RSU existem representantes ainda desconhecidos e/ou não isolados que estão relacionados aos processos de hidrólise, acidogênese e acetogênese na digestão anaeróbia dos resíduos.
ABSTRACT The knowledge of bacterial populations responsible for the biodegradation of solid waste disposed of in landfills can lead to the development of feasible technological alternatives for the treatment and stabilization of residues, resulting in positive impacts for the operation of landfills, energy recovery, public health and the environment. The objective of this work is to characterize the bacterial diversity associated to the biodegradation of grounded MSW in an experimental Cell in the city of Campina Grande-PB. The study covered the stages of construction, statistical planning of the districts of Campina Grande-PB for collection and filling of the experimental Cell with MSW. The DNA samples of the bacteria found in the MSW were extracted with the Power Soil DNA Isolation Kit, then the genetic analysis was performed with universal primers for bacteria via PCR and DGGE, and finally, genetic sequencing (16S RNA region). Following the microbiological examination, the main bacteria associated to the taxons were: Uncultured Pseudomonas sp, Uncultured bacterium, Enterobacter sp., Klebsiella pneumoniae, Uncultured Bacillus sp., suggesting that in the MSW there are still unknown and/or non-isolated representatives that are related to hydrolysis, acidogenesis and acetogenesis in the anaerobic digestion of the residues.
RESUMO
The regulation of the inflammatory response is essential to maintaining homeostasis. Several studies have investigated new drugs that may contribute to avoiding or minimizing excessive inflammatory process. The aim of this study was to evaluate the effect of extracts of green algae Caulerpa mexicana on models inflammation. In mice, the inflammatory peritonitis model is induced by zymosan. Previous treatment of mice with aqueous and methanolic extracts of C. mexicana was able to suppress the cell migration to the peritoneal cavity, in a time-dependent but not in a dose-dependent manner. The treatment of mice with C. mexicana extracts also decreased the xylene-induced ear edema, exerting strong inhibitory leukocyte migration elicited by zymosan into the air pouch. We concluded that administration of the extracts resulted in a reduction of cell migration to different sites as well as a decrease in edema formation induced by chemical irritants. This study demonstrates for the first time the anti-inflammatory effect of aqueous and methanolic extracts from the green marine algae Caulerpa mexicana.
