Risk assessment of lambda-cyhalothrin on aquatic organisms in paddy field in China.

This study was carried out to assess the risk of lambda-cyhalothrin to aquatic organisms used in paddy field, and to provide assistance in the ecological risk management of lambda-cyhalothrin. The acute toxicities of five individual formulations of lambda-cyhalothrin to four aquatic species were investigated in the laboratory, as well as in a simulated paddy field-pond ecosystem, and the results implicated that lambda-cyhalothrin is highly toxic to fish, and to a greater extent to shrimp. There were differences in the toxicities to each aquatic organisms among different formulations. lambda-Cyhalothrin degraded rapidly in the environment, with half-lives of different formulations in paddy field water (0.23-0.53 days), pond water (0.38-0.63 days), and paddy field soil (0.96-7.35 days), respectively. The water overflow from the paddy field following a simulated rainstorm 12h after application of lambda-cyhalothrin did not cause injury to fish, clam or crab, but was severely hazardous to shrimp. Additionally, no injury to shrimp was found when simulated overflow occurred 4 days after application. These results suggest that the environmental risk of lambda-cyhalothrin to aquatic organisms can be reduced by (1) developing a relatively safe formulation such as a suspension concentrate, and/or (2) controlling the drainage time of the paddy field.

How does the European common lizard, Lacerta vivipara, survive the cold of winter?

Although the European common lizard, Lacerta vivipara, is among the most common Eurasian reptile species, we know little about how these lizards cope with very low temperatures. In this study we examined microenvironmental conditions, body temperature, behavior, and cold strategies to see whether strategies of freezing and supercooling, while normally considered to be mutually exclusive, may in fact be adopted simultaneously by the common lizard. Following up on an earlier study of a lowland population, this time we used a mountain population (850 m) to discover differences in overwintering strategies between the two populations. Differential scanning calorimetry conducted during the hibernation period (vs. the activity period) showed that the blood of highland lizards had an increased ability to resist ice formation, confirming an ecophysiological effect most likely mediated by physical properties of the blood. Mean blood glucose level of unfrozen L. vivipara in the field increased significantly (about fourfold) from 8.5+/-0.7 mmol l(-1) in September to 33.2+/-5.6 mmol l(-1) in March. The blood glucose level then experienced a significant decline as it fell to 6. 2+/-0.8 mmol l(-1) after hibernation in April. Glucose, in conclusion, seems to play a role of cryoprotectant rather than antifreeze.

[Study on genotypes of cystathionine beta-synthase in neural tube defects].

Mildly elevated maternal plasma homocysteine (Hcy) levels (hyperhomocysteinemia) have recently been observed in some neural tube defects(NTDs) pregnancies. The aetiology of NTDs is also known to have both genetic and nutritional components. The frequency of two relatively common mutations in the enzyme of cystathionine beta-synthase (CBS), which is one of the main enzymes that controls Hcy level, was examined. Among 76 nonpregnant women in the study, 40 of whom had given birth to infants with NTDs and 36 control women previously had normal offspring. The results showed that no significant difference was found between NTDs mothers and non-NTDs mothers for the frequency of T833-C and the G919-A mutations in the CBS gene. The data suggests that the mother's genotype is not the direct factor involved in the pathogenesis of NTDs.

Osmotic activation of the hypothalamo-neurohypophysial system reversibly downregulates the NMDA receptor subunit, NR2B, in the supraoptic nucleus of the hypothalamus.

NMDA receptor activation produces a characteristic pattern of neuronal firing in magnocellular neuroendocrine cells (MNCs) of the supraoptic nucleus of the hypothalamus (SON) which has been associated with greater hormone release in vivo and in vitro. In addition, i.c.v. administered NMDA receptor blockers suppress the dehydration-induced rise in plasma vasopressin and drinking. To investigate the role of NMDA receptor subunits in the neuroendocrine functions of the magnocellular neuroendocrine cells of the hypothalamus, we examined the effects of osmotic stimulation on the protein expression of the NMDA receptor subunits, NR1 and NR2B, important in binding glycine and glutamate, respectively. Homogenates of SON, paraventricular nucleus of the hypothalamus (PVN), cortex and lateral hypothalamus from control rats and rats given 2% saline water to drink for 4-10 days were subjected to SDS-PAGE and Western blot analysis. This saline water drinking regimen produced a significant rise in plasma osmolality levels. NR1 and NR2B immunoreactivity was detected in SON, PVN, lateral hypothalamus and cortex but not in liver homogenates using subunit-specific polyclonal antibodies and quantified using computer-assisted densitometry. Mean NR2B immunoreactivity was significantly lower in SON (29%) and PVN homogenates (23%) from saline-treated rats than in those from control rats. In addition, the effect of dehydration on NR2B was regionally specific since no significant changes in NR2B expression were observed in homogenates of cortex and lateral hypothalamus. Rehydration allowed recovery of plasma osmolality as well as NR2B protein levels in the SON. These results suggest that changes in NMDA receptor subunit expression contribute to the plasticity manifested by in magnocellular neuroendocrine cells in response to osmotic activation of the hypothalamo-neurohypophysial system. In addition, our results indicate that NMDA receptors on SON and PVN MNCs may contribute to neuroendocrinological functions associated with body fluid homeostasis.

Lateral hypothalamic NMDA receptor subunits NR2A and/or NR2B mediate eating: immunochemical/behavioral evidence.

Cells within the lateral hypothalamic area (LHA) are important in eating control. Glutamate or its analogs, kainic acid (KA) and N-methyl-D-aspartate (NMDA), elicit intense eating when microinjected there, and, conversely, LHA-administered NMDA receptor antagonists suppress deprivation- and NMDA-elicited eating. The subunit composition of LHA NMDA receptors (NMDA-Rs) mediating feeding, however, has not yet been determined. Identifying this is important, because distinct second messengers/modulators may be activated by NMDA-Rs with differing compositions. To begin to address this, we detected LHA NR2A and NR2B subunits by immunoblotting and NR2B subunits by immunohistochemistry using subunit-specific antibodies. To help determine whether NMDA-Rs mediating feeding might contain these subunits, we conducted behavioral studies using LHA-administered ifenprodil, an antagonist selective for NR2A- and/or NR2B-containing NMDA-Rs at the doses we used (0.001-100 nmol). Ifenprodil maximally suppressed NMDA- and deprivation-elicited feeding by 63 and 39%, respectively, but failed to suppress KA-elicited eating, suggesting its actions were behaviorally specific. Collectively, these results suggest that LHA NMDA-Rs, some of which contribute to feeding control, are composed of NR2A and/or NR2B subunits, and implicate NR2A- and/or NR2B-linked signal transduction in feeding behavior.

Developmental plasticity of NR1 and NR2B subunit expression in the supraoptic nucleus of the rat hypothalamus.

Vasopressin and oxytocin neuroendocrine cells within the supraoptic nucleus of the adult hypothalamus (SON) display mRNA expression for the NMDA receptor subunits, NR1 and NR2B, NR2C and NR2D. The NR2B subunit confers slow decay kinetics (relative to NR1/NR2A receptors) and high magnesium sensitivity to NMDA receptor responses--properties which may contribute to the NMDA receptor-mediated bursting manifested by these cells. Therefore, we examined NR2B protein expression and its developmental profile in the SON and compared it to that in the cortex and cerebellum--areas which have been studied previously. We performed Western blot analysis on SON homogenates from embryonic, postnatal (PN7, 14, 21), and adult rats using an NR2B-specific antibody. Adult NR2B levels in the SON and PVN were similar but low relative to those of cortex. SON NR2B protein levels rose in the first postnatal week, remained high through PN21, and later declined to significantly lower levels in the adult. A similar profile was observed in cerebellum, where NR2B expression displayed a sharp peak at PN14 and later declined to minimal or undetectable levels in the adult. In contrast, NR2B continued to be overexpressed through adulthood in the cortex. The ontogenic pattern for NR1 expression, which included unregulation during early postnatal life and adulthood, was similar in the SON and cortex. A different pattern was observed for the cerebellum, where NR1 levels increased gradually after ED17 to reach significantly greater adult levels. Of all three areas studied, the SON displayed the earliest developmental rise in NR1 levels. SON explant cultures proved to be a useful preparation, since they contained neurons which synthesized NR1 and NR2B subunits in quantities similar to those of ED17 SON. Our findings suggest that NMDA receptors on SON neuroendocrine cells are assembled using NR1 and NR2B subunits, and that their plastic expression in early postnatal life may play a role during development.